Integrating AI and CRISPR Cas13a for rapid detection of tomato brown rugose fruit virus

The Tomato Brown Rugose Fruit Virus (ToBRFV) has recently emerged as a serious threat to global tomato production, underscoring the need for rapid and sensitive diagnostic tools. Here, we present an AI-driven CRISPR-Cas13a pipeline for designing crRNAs with high specificity to enable the detection o...

Full description

Saved in:

Bibliographic Details
Published in:	Scientific reports Vol. 15; no. 1; pp. 25422 - 15
Main Authors:	Karimi, Marzieh, Ghorbani, Abozar, Niazi, Ali, Rostami, Mahsa, Tahmasebi, Ahmad
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 14.07.2025 Nature Publishing Group Nature Portfolio
Subjects:	631/208 631/326 631/337 631/61 Automation Bioinformatics Cas13 Computational Biology - methods Conserved sequence CRISPR CRISPR-Cas Systems CrRNA Design Enzymes Fruits Genomes Humanities and Social Sciences Machine learning multidisciplinary Pathogens Plant Diseases - virology Plant viruses Plant Viruses - genetics Plant Viruses - isolation & purification Rapid detection RNA, Viral - genetics Science Science (multidisciplinary) Sensitive diagnostic Solanum lycopersicum - virology ToBRFV Tomatoes Viral infections Viruses ToBRFV Cas13 CrRNA Sensitive diagnostic Rapid detection
ISSN:	2045-2322, 2045-2322
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Be the first to leave a comment!