Potential application of immunoassays for simple, rapid and quantitative detections of phytoavailable neonicotinoid insecticides in cropland soils

This study evaluated the applicability of commercially available kit-based enzyme-linked immunosorbent assay (ELISA) to simple, quick, and quantitative detection for three water-extractable (phytoavailable) neonicotinoid insecticides: dinotefuran, clothianidin, and imidacloprid in soils. ELISA showe...

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Bibliographic Details
Published in:Ecotoxicology and environmental safety Vol. 132; pp. 288 - 294
Main Authors: Watanabe, Eiki, Seike, Nobuyasu, Motoki, Yutaka, Inao, Keiya, Otani, Takashi
Format: Journal Article
Language:English
Published: Netherlands Elsevier Inc 01.10.2016
Subjects:
carbon
clothianidin
cropland
Crops, Agricultural - chemistry
cross reaction
Cross Reactions
detection limit
dinotefuran
Environmental Monitoring - methods
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Guanidines - analysis
imidacloprid
Imidazoles - analysis
Insecticides - analysis
Japan
Matrix interference
Neonicotinoids
Nitro Compounds - analysis
Organic solvent-free analysis
physicochemical properties
Soil - chemistry
Soil Pollutants - analysis
soil sampling
soil water
Soil–water distribution coefficients
Thiazoles - analysis
Water-based extraction
Organic solvent-free analysis
Matrix interference
Soil–water distribution coefficients
Water-based extraction
Enzyme-linked immunosorbent assay
ISSN:0147-6513, 1090-2414, 1090-2414
Online Access:Get full text
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Summary:This study evaluated the applicability of commercially available kit-based enzyme-linked immunosorbent assay (ELISA) to simple, quick, and quantitative detection for three water-extractable (phytoavailable) neonicotinoid insecticides: dinotefuran, clothianidin, and imidacloprid in soils. ELISA showed excellent analytical sensitivity for determination, but with cross-reaction to structurally related neonicotinoid analogues, which might produce false positives. To analyze insecticides in soil samples of diverse physicochemical properties, they were extracted with water. The aqueous soil extracts were assayed directly with ELISA. No matrix interference was observed without additional dilution with water. Recovery experiments for the insecticides from aqueous soil extracts spiked at 2–10 ng/mL showed good accuracy (72–126%) and precision (<16%). Kit-based ELISAs were used to estimate soil–water distribution coefficients (Kd). Values estimated using this method showed positive correlation between organic carbon contents in soil and those for evaluated insecticides. Results indicate that the evaluated kit-based ELISA has applicability for simple, quick, and reliable detection of phytoavailable insecticides in soils and for estimating Kd values in soil. [Display omitted] •ELISAs were applied to the detection for phytoavailable pesticides in soils.•Phytoavailable neonicotinoid insecticides were extracted with water.•Water-extractable insecticides were determined directly without clean-up.•The proposed ELISAs were applied successfully to estimation of Kd values.
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ISSN:0147-6513
1090-2414
1090-2414
DOI:10.1016/j.ecoenv.2016.06.023