Viscoelastic characterization of the human osteosarcoma cancer cell line MG-63 using a fractional-order zener model through automated algorithm design and configuration

Understanding the viscoelastic properties of cells is essential for studying their mechanical behavior and identifying disease-related biomechanical markers. This paper proposes an integrated framework that combines fractional modeling with automated algorithm design to fit force-relaxation data acq...

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Vydáno v:	Scientific reports Ročník 15; číslo 1; s. 31436 - 25
Hlavní autoři:	Duque-Gimenez, Grecia C., Zambrano-Gutierrez, Daniel F., Rodriguez-Nieto, Maricela, Menchaca, Jorge Luis, Cruz-Duarte, Jorge M., Zárate-Triviño, Diana G., Avina-Cervantes, Juan Gabriel, Ortiz-Bayliss, José Carlos
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 26.08.2025 Nature Publishing Group Nature Portfolio
Témata:	639/166/985 639/705/117 Algorithms Atomic force microscopy Automation Biomechanical Phenomena Biomechanics Bone cancer Calculus Cell Line, Tumor Cells Coefficient of variation Design Elasticity Fractional-order zener model Heuristic Humanities and Social Sciences Humans Hyper-heuristic Laplace transforms Mechanical properties Metaheuristic Microscopy, Atomic Force multidisciplinary Optimization Osteosarcoma Osteosarcoma - pathology Parameter estimation Physiology Science Science (multidisciplinary) Statistical analysis Viscoelasticity Viscosity Viscoelasticity Atomic force microscopy Metaheuristic Fractional-order zener model Automated algorithm design Cells Hyper-heuristic
ISSN:	2045-2322, 2045-2322
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Shrnutí:	Understanding the viscoelastic properties of cells is essential for studying their mechanical behavior and identifying disease-related biomechanical markers. This paper proposes an integrated framework that combines fractional modeling with automated algorithm design to fit force-relaxation data acquired through atomic force microscopy. We employ the fractional-order zener model to describe cell relaxation curves and formulate the parameter estimation as a black-box optimization problem. To solve it, we implement a Randomized Constructive Hyper-Heuristic with Local Search (RCHH-LS) that automatically generates tailored metaheuristics (MHs) by exploring combinations of search operators. Our results show that the best-performing MH, composed of two swarm-based dynamics and a local random-walk operator ( ), achieves a performance of , representing a 75% improvement over the mean of all candidate configurations. Subsequent hyperparameter tuning with Optuna reduces this value to , a further 4.7% gain relative to the untuned version while preserving high stability and repeatability. In an evaluation of 21 instances (force-relaxation curves), the tuned provided the best result in 19 cases, achieving an average of , about 12% better than the best two-operator alternative and a coefficient of variation below 0.01%, underscoring its generalization capability. The FOZ model fitted using this solver generalizes well to independent datasets and captures critical viscoelastic parameters. We also confirm that , , and are sensitive to the applied force via a statistical analysis, while remains stable, reinforcing its association with intrinsic cell properties. These results highlight the effectiveness of combining fractional viscoelastic modeling with automated MH design for robust and interpretable mechanical characterization of cells. The proposed approach reduces manual intervention, ensures generalizability, and offers a scalable solution for computational biomechanics.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-025-16174-3