Dopaminergic Projections From the Ventral Tegmental Area to the Nucleus Accumbens Modulate Sevoflurane Anesthesia in Mice

The role of the dopaminergic pathway in general anesthesia and its potential mechanisms are still unknown. In this study, we usedc-Fos staining combined with calcium fiber photometry recording to explore the activity of ventral tegmental area (VTA) dopaminergic neurons(VTA-DA) and nucleus accumbens...

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Published in:Frontiers in cellular neuroscience Vol. 15; p. 671473
Main Authors: Gui, Huan, Liu, Chengxi, He, Haifeng, Zhang, Jie, Chen, Hong, Zhang, Yi
Format: Journal Article
Language:English
Published: Switzerland Frontiers Research Foundation 30.04.2021
Frontiers Media S.A
Subjects:
Anesthesia
c-Fos protein
Calcium
calcium fiber photometry recording
Cellular Neuroscience
Dopamine
Dopamine receptors
dopamine sensors
dopaminergic (DA) neuron
EEG
Electroencephalography
General anesthesia
Genetics
Information processing
Laboratory animals
Life sciences
Neurons
Nucleus accumbens
Optics
Photometry
Righting reflex
Sevoflurane
Sleep
Variance analysis
ventral tegmental area
Ventral tegmentum
Viruses
United States > US
China
dopamine sensors
dopaminergic (DA) neuron
sevoflurane
calcium fiber photometry recording
ventral tegmental area
ISSN:1662-5102, 1662-5102
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Summary:The role of the dopaminergic pathway in general anesthesia and its potential mechanisms are still unknown. In this study, we usedc-Fos staining combined with calcium fiber photometry recording to explore the activity of ventral tegmental area (VTA) dopaminergic neurons(VTA-DA) and nucleus accumbens (NAc) neurons during sevoflurane anesthesia. A genetically encoded dopamine (DA) sensor was used to investigate thefunction of the NAc in sevoflurane anesthesia. Chemogenetics and optogenetics were used to explore the role of the VTA-DA in sevofluraneanesthesia. Electroencephalogram (EEG) spectra, time of loss of righting reflex (LORR) and recovery of righting reflex (RORR) were recorded asassessment indicators. We found that VTA-DA and NAc neurons were inhibited during the induction period and were activated during the recoveryperiod of sevoflurane anesthesia. The fluorescence signals of dopamine decreased in the induction of and increased in the emergence from sevoflurane anesthesia.Activation of VTA-DA and the VTA DA -NAc pathway delayed the induction and facilitated the emergence accompanying with thereduction of delta band and the augmentation of the gamma band. These data demonstrate that VTA-DA neurons play a critical role in modulating sevofluraneanesthesia via the VTA DA -NAc pathway.
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Specialty section: This article was submitted to Cellular Neurophysiology, a section of the journal Frontiers in Cellular Neuroscience
Reviewed by: Yo Oishi, University of Tsukuba, Japan; Christa Van Dort, Harvard Medical School, United States
Edited by: Masahito Yamagata, Harvard University, United States
These authors have contributed equally to this work and share first authorship
ISSN:1662-5102
1662-5102
DOI:10.3389/fncel.2021.671473