A dandelion polysaccharide and its selenium nanoparticles: Structure features and evaluation of anti-tumor activity in zebrafish models

In this study, an inulin fructan (TMP50-2) with moderate anti-tumor activity was obtained from dandelion. To further improve the anti-tumor activity of TMP50-2, a monodisperse and stable spherical nanoparticle (Tw-TMP-SeNP, 50 nm) was fabricated. Physico-chemical analysis revealed that TMP50-2 and T...

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Bibliographic Details
Published in:Carbohydrate Polymers Vol. 270; p. 118365
Main Authors: Zhang, Shaojie, Song, Ziteng, Shi, Lijuan, Zhou, Linan, Zhang, Jie, Cui, Jianlin, Li, Yuhao, Jin, Da-Qing, Ohizumi, Yasushi, Xu, Jing, Guo, Yuanqiang
Format: Journal Article
Language:English
Published: England Elsevier Ltd 15.10.2021
Elsevier BV
Subjects:
A549 Cells
angiogenesis
Animals
Anti-tumor activity
antineoplastic activity
Antineoplastic Agents
Antineoplastic Agents - pharmacology
Apoptosis
Apoptosis - drug effects
Cell Proliferation
Cell Proliferation - drug effects
Danio rerio
dose response
Fructan
Fructans
Fructans - chemistry
Fructans - pharmacology
HeLa Cells
Hep G2 Cells
Humans
hydrogen
Hydrogen Bonding
inulin
Nanoparticles
Nanoparticles - chemistry
Neoplasms
Neoplasms - drug therapy
Neoplasms - metabolism
Neoplasms - pathology
Polysaccharides
Polysaccharides - chemistry
Polysaccharides - pharmacology
polysorbates
Selenium
Selenium - chemistry
Selenium - pharmacology
Selenium nanoparticles
Taraxacum
Taraxacum - chemistry
Taraxacum mongolicum Hand.-Mazz
Zebrafish
Zebrafish
Fructan
Selenium nanoparticles
Taraxacum mongolicum Hand.-Mazz
Anti-tumor activity
ISSN:0144-8617, 1879-1344, 1879-1344
Online Access:Get full text
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Summary:In this study, an inulin fructan (TMP50-2) with moderate anti-tumor activity was obtained from dandelion. To further improve the anti-tumor activity of TMP50-2, a monodisperse and stable spherical nanoparticle (Tw-TMP-SeNP, 50 nm) was fabricated. Physico-chemical analysis revealed that TMP50-2 and Tween 80 were tightly wrapped on the surface of SeNPs by forming CO⋯Se bonds or through hydrogen bonding interaction (OH⋯Se). In vitro anti-tumor assay showed that Tw-TMP-SeNP treatment could significantly inhibit the proliferation of cancer cells (HepG2, A549, and HeLa) in a dose-dependent manner, while HepG2 cells were more susceptible to Tw-TMP-SeNP with an IC50 value of 46.8 μg/mL. The apoptosis induction of HepG2 cells by Tw-TMP-SeNP was evidenced by increasing the proportion of apoptotic cells ranging from 12.5% to 27.4%. Furthermore, in vivo zebrafish model confirmed the anti-tumor activity of Tw-TMP-SeNP by inhibiting the proliferation and migration of tumor cells as well as the angiogenesis of zebrafish embryos. •An inulin fructan, TMP50-2, was purified and characterized from dandelion.•A fructan-based nanoparticle (Tw-TMP-SeNP) was designed, fabricated, and characterized.•Tw-TMP-SeNP exerted anti-tumor activity by inducing apoptosis.•Tw-TMP-SeNP blocked tumor proliferation and migration in the zebrafish xenograft model.•Tw-TMP-SeNP inhibited angiogenesis in the transgenic zebrafish model.
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content type line 23
ISSN:0144-8617
1879-1344
1879-1344
DOI:10.1016/j.carbpol.2021.118365