A non-phenol–chloroform extraction of double-stranded RNA from plant and fungal tissues

Double-stranded RNA (dsRNA) molecules of viruses are found in nature at a very high frequency. Their detection in plants and fungi has been carried out with difficulty due to the complicated dsRNA extraction techniques used commonly which includes phenol–chloroform extractions. In this study, an ext...

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Veröffentlicht in:	Journal of virological methods Jg. 152; H. 1; S. 32 - 37
Hauptverfasser:	Balijja, Alitukiriza, Kvarnheden, Anders, Turchetti, Tullio
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	London Elsevier B.V 01.09.2008 Amsterdam Elsevier New York, NY
Schlagworte:	Biological and medical sciences DNA, Fungal - isolation & purification DNA, Plant - isolation & purification dsRNA extraction dsRNA virus Fundamental and applied biological sciences. Psychology Fungi - virology Microbiology Nicotiana - virology RI-dsRNA RNA Viruses - genetics RNA, Double-Stranded - isolation & purification RNA, Viral - isolation & purification ssRNA virus replicative-intermediate Techniques used in virology Virology dsRNA virus RI-dsRNA dsRNA extraction ssRNA virus replicative-intermediate Double stranded RNA Chloroform Microbiology Phenols Method Virology
ISSN:	0166-0934, 1879-0984
Online-Zugang:	Volltext
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