Relationship of microparticles to progenitor cells as a measure of vascular health in a diabetic population

Objective: Quantitative measures are needed to identify diabetic patients at higher risk for CV events. Cell‐derived microparticles (MPs) are submicron membrane vesicles released from activated cells that are indicative of cell damage. Progenitor cells (PCs) including proangiogenic cells (PACs), oft...

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Vydáno v:	Cytometry. Part B, Clinical cytometry Ročník 78B; číslo 5; s. 329 - 337
Hlavní autoři:	Curtis, Anne M., Zhang, Lifeng, Medenilla, Elizabeth, Gui, Ming, Wilkinson, Patrick F., Hu, Erding, Giri, Jay, Doraiswamy, Vijay, Gunda, Sampath, Burgert, Mark E., Moore, Jonni S, Edelberg, Jay M., Mohler, Emile R.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.09.2010
Témata:	Adult Aged Antigens, CD34 - analysis Antigens, CD34 - metabolism Blood Pressure - drug effects Cell-Derived Microparticles - drug effects Cell-Derived Microparticles - pathology Cholesterol - blood Cohort Studies diabetes Diabetes Mellitus, Type 2 - drug therapy Diabetes Mellitus, Type 2 - pathology Diabetic Angiopathies - drug therapy Diabetic Angiopathies - pathology endothelial Endothelium, Vascular - drug effects Endothelium, Vascular - pathology Enzyme-Linked Immunosorbent Assay Flow Cytometry Humans Hydroxymethylglutaryl-CoA Reductase Inhibitors - therapeutic use Lipoproteins, HDL - blood Lipoproteins, LDL - blood Male microparticles Middle Aged progenitor Stem Cells - drug effects Stem Cells - pathology vascular
ISSN:	1552-4949, 1552-4957, 1552-4957
On-line přístup:	Získat plný text
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Shrnutí:	Objective: Quantitative measures are needed to identify diabetic patients at higher risk for CV events. Cell‐derived microparticles (MPs) are submicron membrane vesicles released from activated cells that are indicative of cell damage. Progenitor cells (PCs) including proangiogenic cells (PACs), often termed endothelial progenitor cells (EPCs), are mediators of reparative capacity. We examined whether the relationship of MPs to PCs/PACs could be used as an improved and clinically feasible index of vascular pathology. Methods and Results: Plasma samples were collected from patients with early‐stage (ES, Diagnosis < 1year) and long‐term (LT, Diagnosis > 5 years,) Type 2 diabetes and compared with age related healthy subjects (H). PC and MP subtypes were measured by a combination of flow cytometry and ELISA‐based methods. The ratio of procoagulant MPs/CD34+ PCs proved a valuable index to distinguish between subject groups (P = 0.01). This index of compromised vascular function was highest in the LT group despite intensive statin therapy and was more informative than a range of soluble protein biomarkers. Conclusions: This is the first report of a relationship between MPs and PCs in Type 2 diabetes. This ratio may provide a quantitative and clinically feasible measurement of vascular dysfunction and cardiovascular risk in patients with diabetes. © 2010 International Clinical Cytometry Society
Bibliografie:	How to cite this article: Curtis AM, Zhang L, Medenilla E, Gui M, Wilkinson PF, Hu E, Giri Y, Doraiswamy V, Gunda S, Burgert ME, Moore JS, Edelberg JM, Mohler ER III. Relationship of microparticles to progenitor cells as a measure of vascular health in a diabetic population. Cytometry Part B 2010; 78B: 329–337. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1552-4949 1552-4957 1552-4957
DOI:	10.1002/cyto.b.20528