Relationship of microparticles to progenitor cells as a measure of vascular health in a diabetic population

Objective: Quantitative measures are needed to identify diabetic patients at higher risk for CV events. Cell‐derived microparticles (MPs) are submicron membrane vesicles released from activated cells that are indicative of cell damage. Progenitor cells (PCs) including proangiogenic cells (PACs), oft...

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Veröffentlicht in:Cytometry. Part B, Clinical cytometry Jg. 78B; H. 5; S. 329 - 337
Hauptverfasser: Curtis, Anne M., Zhang, Lifeng, Medenilla, Elizabeth, Gui, Ming, Wilkinson, Patrick F., Hu, Erding, Giri, Jay, Doraiswamy, Vijay, Gunda, Sampath, Burgert, Mark E., Moore, Jonni S, Edelberg, Jay M., Mohler, Emile R.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.09.2010
Schlagworte:
Adult
Aged
Antigens, CD34 - analysis
Antigens, CD34 - metabolism
Blood Pressure - drug effects
Cell-Derived Microparticles - drug effects
Cell-Derived Microparticles - pathology
Cholesterol - blood
Cohort Studies
diabetes
Diabetes Mellitus, Type 2 - drug therapy
Diabetes Mellitus, Type 2 - pathology
Diabetic Angiopathies - drug therapy
Diabetic Angiopathies - pathology
endothelial
Endothelium, Vascular - drug effects
Endothelium, Vascular - pathology
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Humans
Hydroxymethylglutaryl-CoA Reductase Inhibitors - therapeutic use
Lipoproteins, HDL - blood
Lipoproteins, LDL - blood
Male
microparticles
Middle Aged
progenitor
Stem Cells - drug effects
Stem Cells - pathology
vascular
ISSN:1552-4949, 1552-4957, 1552-4957
Online-Zugang:Volltext
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Zusammenfassung:Objective: Quantitative measures are needed to identify diabetic patients at higher risk for CV events. Cell‐derived microparticles (MPs) are submicron membrane vesicles released from activated cells that are indicative of cell damage. Progenitor cells (PCs) including proangiogenic cells (PACs), often termed endothelial progenitor cells (EPCs), are mediators of reparative capacity. We examined whether the relationship of MPs to PCs/PACs could be used as an improved and clinically feasible index of vascular pathology. Methods and Results: Plasma samples were collected from patients with early‐stage (ES, Diagnosis < 1year) and long‐term (LT, Diagnosis > 5 years,) Type 2 diabetes and compared with age related healthy subjects (H). PC and MP subtypes were measured by a combination of flow cytometry and ELISA‐based methods. The ratio of procoagulant MPs/CD34+ PCs proved a valuable index to distinguish between subject groups (P = 0.01). This index of compromised vascular function was highest in the LT group despite intensive statin therapy and was more informative than a range of soluble protein biomarkers. Conclusions: This is the first report of a relationship between MPs and PCs in Type 2 diabetes. This ratio may provide a quantitative and clinically feasible measurement of vascular dysfunction and cardiovascular risk in patients with diabetes. © 2010 International Clinical Cytometry Society
Bibliographie:How to cite this article: Curtis AM, Zhang L, Medenilla E, Gui M, Wilkinson PF, Hu E, Giri Y, Doraiswamy V, Gunda S, Burgert ME, Moore JS, Edelberg JM, Mohler ER III. Relationship of microparticles to progenitor cells as a measure of vascular health in a diabetic population. Cytometry Part B 2010; 78B: 329–337.
ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1552-4949
1552-4957
1552-4957
DOI:10.1002/cyto.b.20528