Recent advances in quantitative neuroproteomics

•Review article examining quantitative neuroproteomics studies (2008–2013).•We discuss 2D-gel and MS based methods for relative and absolute quantitation.•We discuss proteomic studies of psychiatric diseases and neurodegenerative diseases.•We list neuroproteomic studies in table format according to...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Methods (San Diego, Calif.) Jg. 61; H. 3; S. 186 - 218
Hauptverfasser:	Craft, George E., Chen, Anshu, Nairn, Angus C.
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States Elsevier Inc 15.06.2013
Schlagworte:	algorithms Alzheimer disease Animals bioinformatics brain Central Nervous System - chemistry Central Nervous System - physiology cerebrospinal fluid Chromatography, Liquid - instrumentation Chromatography, Liquid - methods drug abuse electrophoresis Electrophoresis, Gel, Two-Dimensional experimental design Gene Expression Regulation genes Humans Isobaric labeling Label-free quantitation Mass spectrometry Mass Spectrometry - instrumentation Mass Spectrometry - methods Mental Disorders - genetics Mental Disorders - metabolism Mental Disorders - physiopathology Nerve Tissue Proteins - chemistry Nerve Tissue Proteins - genetics Nerve Tissue Proteins - isolation & purification Nerve Tissue Proteins - metabolism Neurodegenerative Diseases - genetics Neurodegenerative Diseases - metabolism Neurodegenerative Diseases - physiopathology neurons Neurons - classification Neurons - metabolism Neurons - physiology Neuropeptides - chemistry Neuropeptides - genetics Neuropeptides - isolation & purification Neuropeptides - metabolism Neuropeptidomics neurophysiology Parkinson disease polyacrylamide protein synthesis Proteomics Proteomics - instrumentation Proteomics - methods Quantitative neuroproteomics Research Design schizophrenia Two-dimensional gel electrophoresis Two-dimensional gel electrophoresis Proteomics Isobaric labeling Label-free quantitation Mass spectrometry Neuropeptidomics Quantitative neuroproteomics
ISSN:	1046-2023, 1095-9130, 1095-9130
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	•Review article examining quantitative neuroproteomics studies (2008–2013).•We discuss 2D-gel and MS based methods for relative and absolute quantitation.•We discuss proteomic studies of psychiatric diseases and neurodegenerative diseases.•We list neuroproteomic studies in table format according to quantitative method utilized.•We discuss future directions and challenges for neuroproteomics studies. The field of proteomics is undergoing rapid development in a number of different areas including improvements in mass spectrometric platforms, peptide identification algorithms and bioinformatics. In particular, new and/or improved approaches have established robust methods that not only allow for in-depth and accurate peptide and protein identification and modification, but also allow for sensitive measurement of relative or absolute quantitation. These methods are beginning to be applied to the area of neuroproteomics, but the central nervous system poses many specific challenges in terms of quantitative proteomics, given the large number of different neuronal cell types that are intermixed and that exhibit distinct patterns of gene and protein expression. This review highlights the recent advances that have been made in quantitative neuroproteomics, with a focus on work published over the last five years that applies emerging methods to normal brain function as well as to various neuropsychiatric disorders including schizophrenia and drug addiction as well as of neurodegenerative diseases including Parkinson’s disease and Alzheimer’s disease. While older methods such as two-dimensional polyacrylamide electrophoresis continued to be used, a variety of more in-depth MS-based approaches including both label (ICAT, iTRAQ, TMT, SILAC, SILAM), label-free (label-free, MRM, SWATH) and absolute quantification methods, are rapidly being applied to neurobiological investigations of normal and diseased brain tissue as well as of cerebrospinal fluid (CSF). While the biological implications of many of these studies remain to be clearly established, that there is a clear need for standardization of experimental design and data analysis, and that the analysis of protein changes in specific neuronal cell types in the central nervous system remains a serious challenge, it appears that the quality and depth of the more recent quantitative proteomics studies is beginning to shed light on a number of aspects of neuroscience that relates to normal brain function as well as of the changes in protein expression and regulation that occurs in neuropsychiatric and neurodegenerative disorders.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 ObjectType-Review-3 content type line 23
ISSN:	1046-2023 1095-9130 1095-9130
DOI:	10.1016/j.ymeth.2013.04.008