Exacerbation of Leishmania (Viannia) shawi infection in BALB/c mice after immunization with soluble antigen from amastigote forms

Passero LFD, da Costa Bordon MLA, de Carvalho AK, Martins LM, Corbett CEP, Laurenti MD. Exacerbation of Leishmania (Viannia) shawi infection in BALB/c mice after immunization with soluble antigen from amastigote forms. APMIS 2010; 118: 973-81. The present study aimed to evaluate the effects of immun...

Celý popis

Uložené v:
Podrobná bibliografia
Vydané v:APMIS : acta pathologica, microbiologica et immunologica Scandinavica Ročník 118; číslo 12; s. 973 - 981
Hlavní autori: PASSERO, LUIZ FELIPE DOMINGUES, DA COSTA BORDON, MARIA LUIZA ANTUNES, DE CARVALHO, ANA KELY, MARTINS, LUCIANO MOURA, CORBETT, CARLOS EDUARDO PEREIRA, LAURENTI, MÁRCIA DALASTRA
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Oxford, UK Blackwell Publishing Ltd 01.12.2010
Blackwell
Predmet:
amastigote antigen
Amastigotes
Animals
Antibodies, Protozoan - blood
Antibodies, Protozoan - immunology
Antigens, Protozoan - immunology
Applied microbiology
Biological and medical sciences
Cell culture
Cytokines - immunology
Fundamental and applied biological sciences. Psychology
gamma -Interferon
Immune response
Immune response (humoral)
Immunization
Immunization - methods
Immunoglobulin G
Infection
Infectious diseases
Interleukin 10
Interleukin 12
Interleukin 4
Leishmania
Leishmania (Viannia) shawi
Leishmania - immunology
Leishmaniasis, Cutaneous - immunology
Leishmaniasis, Cutaneous - parasitology
Leishmaniasis, Cutaneous - prevention & control
Lymph nodes
Male
Medical sciences
Mice
Mice, Inbred BALB C
Microbiology
Nitrate
Nitric Oxide - immunology
Parasites
promastigote antigen
Promastigotes
Skin
Statistics, Nonparametric
Transforming growth factor
Transforming growth factor- beta
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Viannia
Kinetoplastida
Protozoa
Immunization
Rodentia
promastigote antigen
Vaccine
Soluble form
Leishmania (Viannia) shawi
Antigen
Vertebrata
Mammalia
Immunology
Mouse
Leishmania
amastigote antigen
ISSN:0903-4641, 1600-0463, 1600-0463
On-line prístup:Získať plný text
Tagy: Pridať tag
Žiadne tagy, Buďte prvý, kto otaguje tento záznam!
Popis
Shrnutí:Passero LFD, da Costa Bordon MLA, de Carvalho AK, Martins LM, Corbett CEP, Laurenti MD. Exacerbation of Leishmania (Viannia) shawi infection in BALB/c mice after immunization with soluble antigen from amastigote forms. APMIS 2010; 118: 973-81. The present study aimed to evaluate the effects of immunization with soluble amastigote (AmaAg) and promastigote (ProAg) antigens from Leishmania (Viannia) shawi on the course of infection in BALB/c mice. After immunization with AmaAg, the challenged group showed greater lesion size and parasite load in the skin and lymph nodes, associated with diminished interleukin (IL)‐2, IL‐4, IL‐10, interferon (IFN)‐γ and nitrate levels in the supernatant of lymph node cell cultures, together with increases in transforming growth factor (TGF)‐β concentrations and humoral immune response. In contrast, immunization with ProAg led to smaller lesion size with reduced numbers of viable parasites in the skin. Protection was associated with increases in IL‐12, IFN‐γ, TGF‐β and nitrates and decreases in IL‐4 and IL‐10 levels. Concerning humoral immune response, a significant reduction in anti‐leishmania immunoglobulin G was verified in the ProAg‐challenged group. Analysis of these results suggests that AmaAg induced a suppressive cellular immune response in mice, favouring the spread of infection, whereas ProAg induced partial protection associated with increased cellular immune response.
Bibliografia:http://dx.doi.org/10.1111/j.1600-0463.2010.02679.x
ArticleID:APM2679
istex:D0FBC23390824F9BC6833561B233AA72B671814E
ark:/67375/WNG-899HLP3B-N
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0903-4641
1600-0463
1600-0463
DOI:10.1111/j.1600-0463.2010.02679.x