ABNORMAL VASCULAR BUNDLES regulates cell proliferation and procambium cell establishment during aerial organ development in rice

To understand the molecular mechanisms of rice aerial organ development, we identified a mutant gene that caused a significant decrease in the width of aerial organs, termed ABNORMAL VASCULAR BUNDLES (AVB). Histological analysis showed that the slender aerial organs were caused by cell number reduct...

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Published in:The New phytologist Vol. 213; no. 1; pp. 275 - 286
Main Authors: Ma, Ling, Sang, Xianchun, Zhang, Ting, Yu, Zhanyang, Li, Yunfeng, Zhao, Fangming, Wang, Zhongwei, Wang, Yantong, Yu, Peng, Wang, Nan, Zhang, Changwei, Ling, Yinghua, Yang, Zhenglin, He, Guanghua
Format: Journal Article
Language:English
Published: England New Phytologist Trust 01.01.2017
Wiley Subscription Services, Inc
Subjects:
aerial organs
Amino Acid Sequence
auxins
cell division
Cell Division - genetics
Cell Proliferation
Cloning
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
genes
Genes, Plant
genetic complementation
histology
histones
lateral primordia
molecular cloning
mutants
Mutation - genetics
Organogenesis - genetics
Oryza - cytology
Oryza - genetics
Oryza - metabolism
Phenotype
Phylogeny
Plant Proteins - genetics
Plant Proteins - metabolism
ploidy
procambium
Protoplasts - metabolism
rice
rice (Oryza sativa)
sequence analysis
Subcellular Fractions - metabolism
vascular bundles
rice (Oryza sativa)
aerial organs
procambium
cell proliferation
vascular bundles
lateral primordia
ISSN:0028-646X, 1469-8137, 1469-8137
Online Access:Get full text
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Summary:To understand the molecular mechanisms of rice aerial organ development, we identified a mutant gene that caused a significant decrease in the width of aerial organs, termed ABNORMAL VASCULAR BUNDLES (AVB). Histological analysis showed that the slender aerial organs were caused by cell number reduction. In avb, the number of vascular bundles in aerial organs was reduced, whereas the area of the vascular bundles was increased. Ploidy analysis and the in situ expression patterns of histone H4 confirmed that cell proliferation was impaired during lateral primordia development, whereas procambium cells showed a greater ability to undergo cell division in avb. RNA sequencing (RNA-seq) showed that the development process was affected in avb. Map-based cloning and genetic complementation demonstrated that AVB encodes a land plant conserved protein with unknown functions. Our research shows that AVB is involved in the maintenance of the normal cell division pattern in lateral primordia development and that the AVB gene is required for procambium establishment following auxin signaling.
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ISSN:0028-646X
1469-8137
1469-8137
DOI:10.1111/nph.14142