Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches

Macrophages are a heterogeneous cell population involved in tissue homeostasis, inflammation, and various pathologies. Although the major tissue-resident macrophage populations have been extensively studied, interstitial macrophages (IMs) residing within the tissue parenchyma remain poorly defined....

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Published in:Science (American Association for the Advancement of Science) Vol. 363; no. 6432
Main Authors: Chakarov, Svetoslav, Lim, Hwee Ying, Tan, Leonard, Lim, Sheau Yng, See, Peter, Lum, Josephine, Zhang, Xiao-Meng, Foo, Shihui, Nakamizo, Satoshi, Duan, Kaibo, Kong, Wan Ting, Gentek, Rebecca, Balachander, Akhila, Carbajo, Daniel, Bleriot, Camille, Malleret, Benoit, Tam, John Kit Chung, Baig, Sonia, Shabeer, Muhammad, Toh, Sue-Anne Ee Shiow, Schlitzer, Andreas, Larbi, Anis, Marichal, Thomas, Malissen, Bernard, Chen, Jinmiao, Poidinger, Michael, Kabashima, Kenji, Bajenoff, Marc, Ng, Lai Guan, Angeli, Veronique, Ginhoux, Florent
Format: Journal Article
Language:English
Published: United States 15.03.2019
Subjects:
Animals
Antigens, Ly
Cell Lineage
CX3C Chemokine Receptor 1 - genetics
Dermis - immunology
Disease Models, Animal
Fibrosis
Glycoproteins - analysis
Histocompatibility Antigens Class II - genetics
Lung - immunology
Lung - pathology
Macrophages - immunology
Mice
Mice, Inbred C57BL
Monocytes - immunology
Myocardium - immunology
Organic Anion Transporters - genetics
Sequence Analysis, RNA - methods
Single-Cell Analysis - methods
Transcriptome
ISSN:1095-9203, 1095-9203
Online Access:Get more information
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Summary:Macrophages are a heterogeneous cell population involved in tissue homeostasis, inflammation, and various pathologies. Although the major tissue-resident macrophage populations have been extensively studied, interstitial macrophages (IMs) residing within the tissue parenchyma remain poorly defined. Here we studied IMs from murine lung, fat, heart, and dermis. We identified two independent IM subpopulations that are conserved across tissues: Lyve1 MHCII CX3CR1 (Lyve1 MHCII ) and Lyve1 MHCII CX3CR1 (Lyve1 MHCII ) monocyte-derived IMs, with distinct gene expression profiles, phenotypes, functions, and localizations. Using a new mouse model of inducible macrophage depletion ( ), we found that the absence of Lyve1 MHCII IMs exacerbated experimental lung fibrosis. Thus, we demonstrate that two independent populations of IMs coexist across tissues and exhibit conserved niche-dependent functional programming.
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ISSN:1095-9203
1095-9203
DOI:10.1126/science.aau0964