In Vivo Imaging of Dentate Gyrus Mossy Cells in Behaving Mice

Mossy cells in the hilus of the dentate gyrus constitute a major excitatory principal cell type in the mammalian hippocampus; however, it remains unknown how these cells behave in vivo. Here, we have used two-photon Ca imaging to monitor the activity of mossy cells in awake, behaving mice. We find t...

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Bibliographic Details
Published in:Neuron (Cambridge, Mass.) Vol. 93; no. 3; p. 552
Main Authors: Danielson, Nathan B, Turi, Gergely F, Ladow, Max, Chavlis, Spyridon, Petrantonakis, Panagiotis C, Poirazi, Panayiota, Losonczy, Attila
Format: Journal Article
Language:English
Published: United States 08.02.2017
Subjects:
Animals
Behavior, Animal
Calcium - metabolism
Dentate Gyrus - cytology
Dentate Gyrus - metabolism
Mice
Mossy Fibers, Hippocampal - metabolism
Neurons - metabolism
Spatial Navigation - physiology
ISSN:1097-4199, 1097-4199
Online Access:Get more information
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Summary:Mossy cells in the hilus of the dentate gyrus constitute a major excitatory principal cell type in the mammalian hippocampus; however, it remains unknown how these cells behave in vivo. Here, we have used two-photon Ca imaging to monitor the activity of mossy cells in awake, behaving mice. We find that mossy cells are significantly more active than dentate granule cells in vivo, exhibit spatial tuning during head-fixed spatial navigation, and undergo robust remapping of their spatial representations in response to contextual manipulation. Our results provide a functional characterization of mossy cells in the behaving animal and demonstrate their active participation in spatial coding and contextual representation.
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ISSN:1097-4199
1097-4199
DOI:10.1016/j.neuron.2016.12.019