In Vivo Imaging of Dentate Gyrus Mossy Cells in Behaving Mice

Mossy cells in the hilus of the dentate gyrus constitute a major excitatory principal cell type in the mammalian hippocampus; however, it remains unknown how these cells behave in vivo. Here, we have used two-photon Ca imaging to monitor the activity of mossy cells in awake, behaving mice. We find t...

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Veröffentlicht in:Neuron (Cambridge, Mass.) Jg. 93; H. 3; S. 552
Hauptverfasser: Danielson, Nathan B, Turi, Gergely F, Ladow, Max, Chavlis, Spyridon, Petrantonakis, Panagiotis C, Poirazi, Panayiota, Losonczy, Attila
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 08.02.2017
Schlagworte:
Animals
Behavior, Animal
Calcium - metabolism
Dentate Gyrus - cytology
Dentate Gyrus - metabolism
Mice
Mossy Fibers, Hippocampal - metabolism
Neurons - metabolism
Spatial Navigation - physiology
ISSN:1097-4199, 1097-4199
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Abstract Mossy cells in the hilus of the dentate gyrus constitute a major excitatory principal cell type in the mammalian hippocampus; however, it remains unknown how these cells behave in vivo. Here, we have used two-photon Ca imaging to monitor the activity of mossy cells in awake, behaving mice. We find that mossy cells are significantly more active than dentate granule cells in vivo, exhibit spatial tuning during head-fixed spatial navigation, and undergo robust remapping of their spatial representations in response to contextual manipulation. Our results provide a functional characterization of mossy cells in the behaving animal and demonstrate their active participation in spatial coding and contextual representation.
AbstractList Mossy cells in the hilus of the dentate gyrus constitute a major excitatory principal cell type in the mammalian hippocampus; however, it remains unknown how these cells behave in vivo. Here, we have used two-photon Ca2+ imaging to monitor the activity of mossy cells in awake, behaving mice. We find that mossy cells are significantly more active than dentate granule cells in vivo, exhibit spatial tuning during head-fixed spatial navigation, and undergo robust remapping of their spatial representations in response to contextual manipulation. Our results provide a functional characterization of mossy cells in the behaving animal and demonstrate their active participation in spatial coding and contextual representation.Mossy cells in the hilus of the dentate gyrus constitute a major excitatory principal cell type in the mammalian hippocampus; however, it remains unknown how these cells behave in vivo. Here, we have used two-photon Ca2+ imaging to monitor the activity of mossy cells in awake, behaving mice. We find that mossy cells are significantly more active than dentate granule cells in vivo, exhibit spatial tuning during head-fixed spatial navigation, and undergo robust remapping of their spatial representations in response to contextual manipulation. Our results provide a functional characterization of mossy cells in the behaving animal and demonstrate their active participation in spatial coding and contextual representation.
Mossy cells in the hilus of the dentate gyrus constitute a major excitatory principal cell type in the mammalian hippocampus; however, it remains unknown how these cells behave in vivo. Here, we have used two-photon Ca imaging to monitor the activity of mossy cells in awake, behaving mice. We find that mossy cells are significantly more active than dentate granule cells in vivo, exhibit spatial tuning during head-fixed spatial navigation, and undergo robust remapping of their spatial representations in response to contextual manipulation. Our results provide a functional characterization of mossy cells in the behaving animal and demonstrate their active participation in spatial coding and contextual representation.
Author Chavlis, Spyridon
Petrantonakis, Panagiotis C
Turi, Gergely F
Poirazi, Panayiota
Ladow, Max
Losonczy, Attila
Danielson, Nathan B
Author_xml – sequence: 1
  givenname: Nathan B
  surname: Danielson
  fullname: Danielson, Nathan B
  organization: Department of Neuroscience, Columbia University, New York, NY 10032, USA; Doctoral Program in Neurobiology and Behavior, Columbia University, New York, NY 10032, USA
– sequence: 2
  givenname: Gergely F
  surname: Turi
  fullname: Turi, Gergely F
  organization: Department of Neuroscience, Columbia University, New York, NY 10032, USA
– sequence: 3
  givenname: Max
  surname: Ladow
  fullname: Ladow, Max
  organization: Department of Neuroscience, Columbia University, New York, NY 10032, USA
– sequence: 4
  givenname: Spyridon
  surname: Chavlis
  fullname: Chavlis, Spyridon
  organization: Institute of Molecular Biology and Biotechnology (IMBB), Foundation for Research and Technology - Hellas (FORTH), 700 13 Heraklion, Crete, Greece; Department of Biology, School of Sciences and Engineering, University of Crete, 741 00 Heraklion, Crete, Greece, Columbia University, New York, NY 10032, USA
– sequence: 5
  givenname: Panagiotis C
  surname: Petrantonakis
  fullname: Petrantonakis, Panagiotis C
  organization: Institute of Molecular Biology and Biotechnology (IMBB), Foundation for Research and Technology - Hellas (FORTH), 700 13 Heraklion, Crete, Greece
– sequence: 6
  givenname: Panayiota
  surname: Poirazi
  fullname: Poirazi, Panayiota
  email: poirazi@imbb.forth.gr
  organization: Institute of Molecular Biology and Biotechnology (IMBB), Foundation for Research and Technology - Hellas (FORTH), 700 13 Heraklion, Crete, Greece. Electronic address: poirazi@imbb.forth.gr
– sequence: 7
  givenname: Attila
  surname: Losonczy
  fullname: Losonczy, Attila
  email: al2856@cumc.columbia.edu
  organization: Department of Neuroscience, Columbia University, New York, NY 10032, USA; Kavli Institute for Brain Science, Columbia University, New York, NY 10032, USA; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY 10032, USA. Electronic address: al2856@cumc.columbia.edu
BackLink https://www.ncbi.nlm.nih.gov/pubmed/28132825$$D View this record in MEDLINE/PubMed
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Snippet Mossy cells in the hilus of the dentate gyrus constitute a major excitatory principal cell type in the mammalian hippocampus; however, it remains unknown how...
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SubjectTerms Animals
Behavior, Animal
Calcium - metabolism
Dentate Gyrus - cytology
Dentate Gyrus - metabolism
Mice
Mossy Fibers, Hippocampal - metabolism
Neurons - metabolism
Spatial Navigation - physiology
Title In Vivo Imaging of Dentate Gyrus Mossy Cells in Behaving Mice
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