The Subgingival Microbiome of Periodontal Pockets With Different Probing Depths in Chronic and Aggressive Periodontitis: A Pilot Study

Periodontitis is a kind of infectious disease initiated by colonization of subgingival periodontal pathogens, which cause destruction of tooth-supporting tissues, and is a predominant threat to oral health as the most common cause of loss of teeth. The aim of this pilot study was to characterize the...

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Bibliographic Details
Published in:Frontiers in cellular and infection microbiology Vol. 8; p. 124
Main Authors: Shi, Meng, Wei, Yiping, Hu, Wenjie, Nie, Yong, Wu, Xiaolei, Lu, Ruifang
Format: Journal Article
Language:English
Published: Switzerland Frontiers Media SA 01.05.2018
Frontiers Media S.A
Subjects:
16S rDNA
Adult
aggressive periodontitis
Aggressive Periodontitis - microbiology
Bacteria
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Biodiversity
Biofilms
chronic periodontitis
Chronic Periodontitis - microbiology
Corynebacterium
Dental Plaque - microbiology
DNA, Bacterial - genetics
Female
Genetic testing
Gingiva
Gingiva - microbiology
Gum disease
High-Throughput Nucleotide Sequencing
Humans
Infectious diseases
Male
Microbiology
Microbiomes
Microbiota - genetics
Middle Aged
Mouth
Next-generation sequencing
Pathogenesis
Periodontal Pocket - microbiology
Periodontics
Periodontitis
Pilot Projects
RNA, Ribosomal, 16S - genetics
rRNA 16S
subgingival microbiome
Teeth
United States > US
subgingival microbiome
aggressive periodontitis
16S rDNA
high-throughput nucleotide sequencing
chronic periodontitis
ISSN:2235-2988, 2235-2988
Online Access:Get full text
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Summary:Periodontitis is a kind of infectious disease initiated by colonization of subgingival periodontal pathogens, which cause destruction of tooth-supporting tissues, and is a predominant threat to oral health as the most common cause of loss of teeth. The aim of this pilot study was to characterize the subgingival bacterial biodiversity of periodontal pockets with different probing depths in patients with different forms of periodontitis. Twenty-one subgingival plaque samples were collected from three patients with chronic periodontitis (ChP), three patients with aggressive periodontitis (AgP) and three periodontally healthy subjects (PH). Each patient with periodontitis was sampled at three sites, at different probing depths (PDs, one each at 4 mm, 5-6 mm, and ≥ 7 mm). Using 16S rRNA gene high-throughput sequencing and bioinformatic analysis, we found that subgingival communities in health and periodontitis samples largely differed. Meanwhile, , and increased with the deepening of the pockets in ChP, whilst only was negatively associated with PD. In AgP, and were positively associated with PD, while , and were negatively associated with PD. And among these two groups, shifted differently. Moreover, in subgingival plaque, the unweighted UniFrac distances between samples from pockets with different PD in the same patients were significantly lower than those from pockets within the same PD category from different patients. This study demonstrated the shift of the subgingival microbiome in individual teeth sites during disease development. Within the limitation of the relative small sample size, this pilot study shed new light on the dynamic relationship between the extent of periodontal destruction and the subgingival microbiome.
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Edited by: Ulvi Kahraman Gürsoy, University of Turku, Finland
Reviewed by: Guliz N. Guncu, Hacettepe University, Turkey; Başak Dogan, Marmara University, Turkey
These authors have contributed equally to this work.
ISSN:2235-2988
2235-2988
DOI:10.3389/fcimb.2018.00124