The Subgingival Microbiome of Periodontal Pockets With Different Probing Depths in Chronic and Aggressive Periodontitis: A Pilot Study

Periodontitis is a kind of infectious disease initiated by colonization of subgingival periodontal pathogens, which cause destruction of tooth-supporting tissues, and is a predominant threat to oral health as the most common cause of loss of teeth. The aim of this pilot study was to characterize the...

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Vydáno v:	Frontiers in cellular and infection microbiology Ročník 8; s. 124
Hlavní autoři:	Shi, Meng, Wei, Yiping, Hu, Wenjie, Nie, Yong, Wu, Xiaolei, Lu, Ruifang
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Switzerland Frontiers Media SA 01.05.2018 Frontiers Media S.A
Témata:	16S rDNA Adult aggressive periodontitis Aggressive Periodontitis - microbiology Bacteria Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Biodiversity Biofilms chronic periodontitis Chronic Periodontitis - microbiology Corynebacterium Dental Plaque - microbiology DNA, Bacterial - genetics Female Genetic testing Gingiva Gingiva - microbiology Gum disease High-Throughput Nucleotide Sequencing Humans Infectious diseases Male Microbiology Microbiomes Microbiota - genetics Middle Aged Mouth Next-generation sequencing Pathogenesis Periodontal Pocket - microbiology Periodontics Periodontitis Pilot Projects RNA, Ribosomal, 16S - genetics rRNA 16S subgingival microbiome Teeth United States > US subgingival microbiome aggressive periodontitis 16S rDNA high-throughput nucleotide sequencing chronic periodontitis
ISSN:	2235-2988, 2235-2988
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Shrnutí:	Periodontitis is a kind of infectious disease initiated by colonization of subgingival periodontal pathogens, which cause destruction of tooth-supporting tissues, and is a predominant threat to oral health as the most common cause of loss of teeth. The aim of this pilot study was to characterize the subgingival bacterial biodiversity of periodontal pockets with different probing depths in patients with different forms of periodontitis. Twenty-one subgingival plaque samples were collected from three patients with chronic periodontitis (ChP), three patients with aggressive periodontitis (AgP) and three periodontally healthy subjects (PH). Each patient with periodontitis was sampled at three sites, at different probing depths (PDs, one each at 4 mm, 5-6 mm, and ≥ 7 mm). Using 16S rRNA gene high-throughput sequencing and bioinformatic analysis, we found that subgingival communities in health and periodontitis samples largely differed. Meanwhile, , and increased with the deepening of the pockets in ChP, whilst only was negatively associated with PD. In AgP, and were positively associated with PD, while , and were negatively associated with PD. And among these two groups, shifted differently. Moreover, in subgingival plaque, the unweighted UniFrac distances between samples from pockets with different PD in the same patients were significantly lower than those from pockets within the same PD category from different patients. This study demonstrated the shift of the subgingival microbiome in individual teeth sites during disease development. Within the limitation of the relative small sample size, this pilot study shed new light on the dynamic relationship between the extent of periodontal destruction and the subgingival microbiome.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Edited by: Ulvi Kahraman Gürsoy, University of Turku, Finland Reviewed by: Guliz N. Guncu, Hacettepe University, Turkey; Başak Dogan, Marmara University, Turkey These authors have contributed equally to this work.
ISSN:	2235-2988 2235-2988
DOI:	10.3389/fcimb.2018.00124