Connecting imaging mass spectrometry and magnetic resonance imaging-based anatomical atlases for automated anatomical interpretation and differential analysis

Imaging mass spectrometry (IMS) is a molecular imaging technology that can measure thousands of biomolecules concurrently without prior tagging, making it particularly suitable for exploratory research. However, the data size and dimensionality often makes thorough extraction of relevant information...

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Veröffentlicht in:Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics Jg. 1865; H. 7; S. 967 - 977
Hauptverfasser: Verbeeck, Nico, Spraggins, Jeffrey M., Murphy, Monika J.M., Wang, Hui-dong, Deutch, Ariel Y., Caprioli, Richard M., Van de Plas, Raf
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Netherlands Elsevier B.V 01.07.2017
Elsevier BV
Schlagworte:
Anatomical atlas
animal models
Animals
biochemical compounds
Brain
Brain - anatomy & histology
Brain - metabolism
Brain - pathology
FTICR MS
image analysis
Image Processing, Computer-Assisted
Image Processing, Computer-Assisted - methods
Imaging mass spectrometry
Imaging, Three-Dimensional
Imaging, Three-Dimensional - methods
Ions
Ions - metabolism
Magnetic Resonance Imaging
Magnetic Resonance Imaging - methods
Male
Mass Spectrometry
Mass Spectrometry - methods
Mice
MRI
Parkinson Disease
Parkinson Disease - pathology
Rat brain
Rats
Rats, Sprague-Dawley
SBA
PD
AMBA
MRI
Imaging mass spectrometry
t-SNE
FTICR MS
FTICR
Rat brain
PCA
Anatomical atlas
ISSN:1570-9639, 1878-1454
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Zusammenfassung:Imaging mass spectrometry (IMS) is a molecular imaging technology that can measure thousands of biomolecules concurrently without prior tagging, making it particularly suitable for exploratory research. However, the data size and dimensionality often makes thorough extraction of relevant information impractical. To help guide and accelerate IMS data analysis, we recently developed a framework that integrates IMS measurements with anatomical atlases, opening up opportunities for anatomy-driven exploration of IMS data. One example is the automated anatomical interpretation of ion images, where empirically measured ion distributions are automatically decomposed into their underlying anatomical structures. While offering significant potential, IMS-atlas integration has thus far been restricted to the Allen Mouse Brain Atlas (AMBA) and mouse brain samples. Here, we expand the applicability of this framework by extending towards new animal species and a new set of anatomical atlases retrieved from the Scalable Brain Atlas (SBA). Furthermore, as many SBA atlases are based on magnetic resonance imaging (MRI) data, a new registration pipeline was developed that enables direct non-rigid IMS-to-MRI registration. These developments are demonstrated on protein-focused FTICR IMS measurements from coronal brain sections of a Parkinson's disease (PD) rat model. The measurements are integrated with an MRI-based rat brain atlas from the SBA. The new rat-focused IMS-atlas integration is used to perform automated anatomical interpretation and to find differential ions between healthy and diseased tissue. IMS-atlas integration can serve as an important accelerator in IMS data exploration, and with these new developments it can now be applied to a wider variety of animal species and modalities. This article is part of a Special Issue entitled: MALDI Imaging, edited by Dr. Corinna Henkel and Prof. Peter Hoffmann.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1570-9639
1878-1454
DOI:10.1016/j.bbapap.2017.02.016