Retrogradely Transportable Lentivirus Tracers for Mapping Spinal Cord Locomotor Circuits

Retrograde tracing is a key facet of neuroanatomical studies involving long distance projection neurons. Previous groups have utilized a variety of tools ranging from classical chemical tracers to newer methods employing viruses for gene delivery. Here, we highlight the usage of a lentivirus that pe...

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Published in:Frontiers in neural circuits Vol. 12; p. 60
Main Authors: Sheikh, Imran S., Keefe, Kathleen M., Sterling, Noelle A., Junker, Ian P., Eneanya, Chidubem I., Liu, Yingpeng, Tang, Xiao-Qing, Smith, George M.
Format: Journal Article
Language:English
Published: Switzerland Frontiers Research Foundation 25.07.2018
Frontiers Media S.A
Subjects:
Anatomy
Animals
Axonal Transport - physiology
Brain architecture
Circuits
corticospinal
Disease Models, Animal
Female
Forelimb - physiology
Gene transfer
Glycoproteins
Hindlimb - physiology
HIV
Human immunodeficiency virus
Interneurons - physiology
Labeling
Laboratory animals
Lentivirus
Locomotion - physiology
Motor Neurons - physiology
Nervous system
Neural Pathways - physiology
Neural Pathways - physiopathology
Neuroscience
Presynapse
propriospinal
Rabies
Rats
Rats, Sprague-Dawley
Recovery of Function - physiology
reticulospinal
retrograde tracing
Retrograde transport
Rodents
rubrospinal
Spinal cord
Spinal Cord - physiology
Spinal cord injuries
Spinal Cord Injuries - physiopathology
Thorax
Tracers
Tropical diseases
Vectors (Biology)
Viruses
reticulospinal
rubrospinal
spontaneous recovery
corticospinal
propriospinal
spinal cord
lentivirus
retrograde tracing
ISSN:1662-5110, 1662-5110
Online Access:Get full text
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Summary:Retrograde tracing is a key facet of neuroanatomical studies involving long distance projection neurons. Previous groups have utilized a variety of tools ranging from classical chemical tracers to newer methods employing viruses for gene delivery. Here, we highlight the usage of a lentivirus that permits highly efficient retrograde transport (HiRet) from synaptic terminals within the cervical and lumbar enlargements of the spinal cord. By injecting HiRet, we can clearly identify supraspinal and propriospinal circuits innervating motor neuron pools relating to forelimb and hindlimb function. We observed robust labeling of propriospinal neurons, including high fidelity details of dendritic arbors and axon terminals seldom seen with chemical tracers. In addition, we examine changes in interneuronal circuits occurring after a thoracic contusion, highlighting populations that potentially contribute to spontaneous behavioral recovery in this lesion model. Our study demonstrates that the HiRet lentivirus is a unique tool for examining neuronal circuitry within the brain and spinal cord.
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These authors have contributed equally to this work.
Reviewed by: Marco Beato, University College London, United Kingdom; Arshad M. Khan, The University of Texas at El Paso, United States
Edited by: Edward S. Ruthazer, McGill University, Canada
ISSN:1662-5110
1662-5110
DOI:10.3389/fncir.2018.00060