Diagnosis of ischemic small bowel disease by measurement of serum intestinal fatty acid-binding protein in patients with acute abdomen : a multicenter, observer-blinded validation study

Background Intestinal fatty acid-binding protein (I-FABP) is a low-molecular-mass (15 kDa) cytosolic protein found exclusively in the epithelial cells of the small bowel mucosa. We aimed to evaluate the clinical usefulness of serum I-FABP measurement for the diagnosis of ischemic small bowel disease...

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Vydané v:Journal of gastroenterology Ročník 46; číslo 4; s. 492 - 500
Hlavní autori: Kanda, Tatsuo, Tsukahara, Akihiro, Ueki, Kyo, Sakai, Yasuo, Tani, Tatsuo, Nishimura, Atsushi, Yamazaki, Toshiyuki, Tamiya, Yoichi, Tada, Tetsuya, Hirota, Masaki, Hasegawa, Jun, Funaoka, Hiroyuki, Fujii, Hiroshi, Hatakeyama, Katsuyoshi
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Japan Springer Japan 01.04.2011
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Springer Nature B.V
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ISSN:0944-1174, 1435-5922, 1435-5922
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Abstract Background Intestinal fatty acid-binding protein (I-FABP) is a low-molecular-mass (15 kDa) cytosolic protein found exclusively in the epithelial cells of the small bowel mucosa. We aimed to evaluate the clinical usefulness of serum I-FABP measurement for the diagnosis of ischemic small bowel disease. Methods Patients with a clinical diagnosis of acute abdomen were recruited for this multicenter trial at one university hospital and nine city hospitals over a 13-month period. Serum I-FABP levels were measured in 361 eligible patients by an enzyme-linked immunosorbent assay using a specific monoclonal antibody. Results Of the 361 patients, 242 underwent surgery, and small bowel ischemia was diagnosed in 52 patients. The mean serum I-FABP level in the patients with small bowel ischemia was 40.7 ± 117.9 ng/ml, which was significantly higher than that in patients with non-ischemic small bowel disease (5.8 ± 15.6 ng/ml) and those with non-small bowel disease (1.8 ± 1.7 ng/ml). The serum I-FABP cutoff level for the diagnosis of small bowel ischemia was 3.1 ng/ml. Serum I-FABP was more efficient than conventional biochemical markers, in terms of sensitivity and positive and negative predictive values, in the diagnosis of small bowel ischemia. However, its specificity was slightly lower than that of creatinine phosphokinase or lactate dehydrogenase. The positive and negative likelihood ratios of serum I-FABP were 3.01 and 0.29, respectively. Conclusion Serum I-FABP measurement is a non-invasive method that is potentially useful for the efficient identification of patients with acute abdomen who are at risk of small bowel ischemia.
AbstractList Intestinal fatty acid-binding protein (I-FABP) is a low-molecular-mass (15 kDa) cytosolic protein found exclusively in the epithelial cells of the small bowel mucosa. We aimed to evaluate the clinical usefulness of serum I-FABP measurement for the diagnosis of ischemic small bowel disease.BACKGROUNDIntestinal fatty acid-binding protein (I-FABP) is a low-molecular-mass (15 kDa) cytosolic protein found exclusively in the epithelial cells of the small bowel mucosa. We aimed to evaluate the clinical usefulness of serum I-FABP measurement for the diagnosis of ischemic small bowel disease.Patients with a clinical diagnosis of acute abdomen were recruited for this multicenter trial at one university hospital and nine city hospitals over a 13-month period. Serum I-FABP levels were measured in 361 eligible patients by an enzyme-linked immunosorbent assay using a specific monoclonal antibody.METHODSPatients with a clinical diagnosis of acute abdomen were recruited for this multicenter trial at one university hospital and nine city hospitals over a 13-month period. Serum I-FABP levels were measured in 361 eligible patients by an enzyme-linked immunosorbent assay using a specific monoclonal antibody.Of the 361 patients, 242 underwent surgery, and small bowel ischemia was diagnosed in 52 patients. The mean serum I-FABP level in the patients with small bowel ischemia was 40.7 ± 117.9 ng/ml, which was significantly higher than that in patients with non-ischemic small bowel disease (5.8 ± 15.6 ng/ml) and those with non-small bowel disease (1.8 ± 1.7 ng/ml). The serum I-FABP cutoff level for the diagnosis of small bowel ischemia was 3.1 ng/ml. Serum I-FABP was more efficient than conventional biochemical markers, in terms of sensitivity and positive and negative predictive values, in the diagnosis of small bowel ischemia. However, its specificity was slightly lower than that of creatinine phosphokinase or lactate dehydrogenase. The positive and negative likelihood ratios of serum I-FABP were 3.01 and 0.29, respectively.RESULTSOf the 361 patients, 242 underwent surgery, and small bowel ischemia was diagnosed in 52 patients. The mean serum I-FABP level in the patients with small bowel ischemia was 40.7 ± 117.9 ng/ml, which was significantly higher than that in patients with non-ischemic small bowel disease (5.8 ± 15.6 ng/ml) and those with non-small bowel disease (1.8 ± 1.7 ng/ml). The serum I-FABP cutoff level for the diagnosis of small bowel ischemia was 3.1 ng/ml. Serum I-FABP was more efficient than conventional biochemical markers, in terms of sensitivity and positive and negative predictive values, in the diagnosis of small bowel ischemia. However, its specificity was slightly lower than that of creatinine phosphokinase or lactate dehydrogenase. The positive and negative likelihood ratios of serum I-FABP were 3.01 and 0.29, respectively.Serum I-FABP measurement is a non-invasive method that is potentially useful for the efficient identification of patients with acute abdomen who are at risk of small bowel ischemia.CONCLUSIONSerum I-FABP measurement is a non-invasive method that is potentially useful for the efficient identification of patients with acute abdomen who are at risk of small bowel ischemia.
Intestinal fatty acid-binding protein (I-FABP) is a low-molecular-mass (15 kDa) cytosolic protein found exclusively in the epithelial cells of the small bowel mucosa. We aimed to evaluate the clinical usefulness of serum I-FABP measurement for the diagnosis of ischemic small bowel disease. Patients with a clinical diagnosis of acute abdomen were recruited for this multicenter trial at one university hospital and nine city hospitals over a 13-month period. Serum I-FABP levels were measured in 361 eligible patients by an enzyme-linked immunosorbent assay using a specific monoclonal antibody. Of the 361 patients, 242 underwent surgery, and small bowel ischemia was diagnosed in 52 patients. The mean serum I-FABP level in the patients with small bowel ischemia was 40.7 ± 117.9 ng/ml, which was significantly higher than that in patients with non-ischemic small bowel disease (5.8 ± 15.6 ng/ml) and those with non-small bowel disease (1.8 ± 1.7 ng/ml). The serum I-FABP cutoff level for the diagnosis of small bowel ischemia was 3.1 ng/ml. Serum I-FABP was more efficient than conventional biochemical markers, in terms of sensitivity and positive and negative predictive values, in the diagnosis of small bowel ischemia. However, its specificity was slightly lower than that of creatinine phosphokinase or lactate dehydrogenase. The positive and negative likelihood ratios of serum I-FABP were 3.01 and 0.29, respectively. Serum I-FABP measurement is a non-invasive method that is potentially useful for the efficient identification of patients with acute abdomen who are at risk of small bowel ischemia.[PUBLICATION ABSTRACT]
Intestinal fatty acid-binding protein (I-FABP) is a low-molecular-mass (15 kDa) cytosolic protein found exclusively in the epithelial cells of the small bowel mucosa. We aimed to evaluate the clinical usefulness of serum I-FABP measurement for the diagnosis of ischemic small bowel disease. Patients with a clinical diagnosis of acute abdomen were recruited for this multicenter trial at one university hospital and nine city hospitals over a 13-month period. Serum I-FABP levels were measured in 361 eligible patients by an enzyme-linked immunosorbent assay using a specific monoclonal antibody. Of the 361 patients, 242 underwent surgery, and small bowel ischemia was diagnosed in 52 patients. The mean serum I-FABP level in the patients with small bowel ischemia was 40.7 ± 117.9 ng/ml, which was significantly higher than that in patients with non-ischemic small bowel disease (5.8 ± 15.6 ng/ml) and those with non-small bowel disease (1.8 ± 1.7 ng/ml). The serum I-FABP cutoff level for the diagnosis of small bowel ischemia was 3.1 ng/ml. Serum I-FABP was more efficient than conventional biochemical markers, in terms of sensitivity and positive and negative predictive values, in the diagnosis of small bowel ischemia. However, its specificity was slightly lower than that of creatinine phosphokinase or lactate dehydrogenase. The positive and negative likelihood ratios of serum I-FABP were 3.01 and 0.29, respectively. Serum I-FABP measurement is a non-invasive method that is potentially useful for the efficient identification of patients with acute abdomen who are at risk of small bowel ischemia.
Intestinal fatty acid-binding protein (I-FABP) is a low-molecular-mass (15 kDa) cytosolic protein found exclusively in the epithelial cells of the small bowel mucosa. We aimed to evaluate the clinical usefulness of serum I-FABP measurement for the diagnosis of ischemic small bowel disease. Of the 361 patients, 242 underwent surgery, and small bowel ischemia was diagnosed in 52 patients. The mean serum I-FABP level in the patients with small bowel ischemia was 40.7 ± 117.9 ng/ml, which was significantly higher than that in patients with non-ischemic small bowel disease (5.8 ± 15.6 ng/ml) and those with non-small bowel disease (1.8 ± 1.7 ng/ml). The serum I-FABP cutoff level for the diagnosis of small bowel ischemia was 3.1 ng/ml. Serum I-FABP was more efficient than conventional biochemical markers, in terms of sensitivity and positive and negative predictive values, in the diagnosis of small bowel ischemia. However, its specificity was slightly lower than that of creatinine phosphokinase or lactate dehydrogenase. The positive and negative likelihood ratios of serum I-FABP were 3.01 and 0.29, respectively. Serum I-FABP measurement is a non-invasive method that is potentially useful for the efficient identification of patients with acute abdomen who are at risk of small bowel ischemia.
Background Intestinal fatty acid-binding protein (I-FABP) is a low-molecular-mass (15 kDa) cytosolic protein found exclusively in the epithelial cells of the small bowel mucosa. We aimed to evaluate the clinical usefulness of serum I-FABP measurement for the diagnosis of ischemic small bowel disease. Methods Patients with a clinical diagnosis of acute abdomen were recruited for this multicenter trial at one university hospital and nine city hospitals over a 13-month period. Serum I-FABP levels were measured in 361 eligible patients by an enzyme-linked immunosorbent assay using a specific monoclonal antibody. Results Of the 361 patients, 242 underwent surgery, and small bowel ischemia was diagnosed in 52 patients. The mean serum I-FABP level in the patients with small bowel ischemia was 40.7 ± 117.9 ng/ml, which was significantly higher than that in patients with non-ischemic small bowel disease (5.8 ± 15.6 ng/ml) and those with non-small bowel disease (1.8 ± 1.7 ng/ml). The serum I-FABP cutoff level for the diagnosis of small bowel ischemia was 3.1 ng/ml. Serum I-FABP was more efficient than conventional biochemical markers, in terms of sensitivity and positive and negative predictive values, in the diagnosis of small bowel ischemia. However, its specificity was slightly lower than that of creatinine phosphokinase or lactate dehydrogenase. The positive and negative likelihood ratios of serum I-FABP were 3.01 and 0.29, respectively. Conclusion Serum I-FABP measurement is a non-invasive method that is potentially useful for the efficient identification of patients with acute abdomen who are at risk of small bowel ischemia.
Background Intestinal fatty acid-binding protein (I-FABP) is a low-molecular-mass (15 kDa) cytosolic protein found exclusively in the epithelial cells of the small bowel mucosa. We aimed to evaluate the clinical usefulness of serum I-FABP measurement for the diagnosis of ischemic small bowel disease. Methods Patients with a clinical diagnosis of acute abdomen were recruited for this multicenter trial at one university hospital and nine city hospitals over a 13-month period. Serum I-FABP levels were measured in 361 eligible patients by an enzyme-linked immunosorbent assay using a specific monoclonal antibody. Results Of the 361 patients, 242 underwent surgery, and small bowel ischemia was diagnosed in 52 patients. The mean serum I-FABP level in the patients with small bowel ischemia was 40.7 ± 117.9 ng/ml, which was significantly higher than that in patients with non-ischemic small bowel disease (5.8 ± 15.6 ng/ml) and those with non-small bowel disease (1.8 ± 1.7 ng/ml). The serum I-FABP cutoff level for the diagnosis of small bowel ischemia was 3.1 ng/ml. Serum I-FABP was more efficient than conventional biochemical markers, in terms of sensitivity and positive and negative predictive values, in the diagnosis of small bowel ischemia. However, its specificity was slightly lower than that of creatinine phosphokinase or lactate dehydrogenase. The positive and negative likelihood ratios of serum I-FABP were 3.01 and 0.29, respectively. Conclusion Serum I-FABP measurement is a non-invasive method that is potentially useful for the efficient identification of patients with acute abdomen who are at risk of small bowel ischemia.
Audience Academic
Author HATAKEYAMA Katsuyoshi
TSUKAHARA Akihiro
YAMAZAKI Toshiyuki
UEKI Kyo
FUNAOKA Hiroyuki
SAKAI Yasuo
KANDA Tatsuo
HIROTA Masaki
TANI Tatsuo
TADA Tetsuya
NISHIMURA Atsushi
HASEGAWA Jun
TAMIYA Yoichi
FUJII Hiroshi
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  givenname: Tatsuo
  surname: Kanda
  fullname: Kanda, Tatsuo
  email: kandat@med.niigata-u.ac.jp
  organization: Division of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences
– sequence: 2
  givenname: Akihiro
  surname: Tsukahara
  fullname: Tsukahara, Akihiro
  organization: Department of Surgery, Niigata Prefecture Shibata Hospital
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  surname: Ueki
  fullname: Ueki, Kyo
  organization: Department of Surgery, Kariwagun General Hospital
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  organization: Department of Surgery, Saiseikai Niigata Daini Hospital
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  surname: Tani
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  organization: Division of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences
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  givenname: Atsushi
  surname: Nishimura
  fullname: Nishimura, Atsushi
  organization: Department of Surgery, Nagaoka Chuo General Hospital
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  surname: Yamazaki
  fullname: Yamazaki, Toshiyuki
  organization: Department of Surgery, Niigata City General Hospital
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  givenname: Yoichi
  surname: Tamiya
  fullname: Tamiya, Yoichi
  organization: Department of Surgery, Niigata Prefecture Yoshida Hospital
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  givenname: Tetsuya
  surname: Tada
  fullname: Tada, Tetsuya
  organization: Department of Surgery, Tachikawa General Hospital
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  givenname: Masaki
  surname: Hirota
  fullname: Hirota, Masaki
  organization: Department of Surgery, Niigata Prefecture Muikamachi Hospital
– sequence: 11
  givenname: Jun
  surname: Hasegawa
  fullname: Hasegawa, Jun
  organization: Department of Surgery, Japanese Red Cross Nagaoka Hospital
– sequence: 12
  givenname: Hiroyuki
  surname: Funaoka
  fullname: Funaoka, Hiroyuki
  organization: DS Pharma Biomedical Co., Ltd
– sequence: 13
  givenname: Hiroshi
  surname: Fujii
  fullname: Fujii, Hiroshi
  organization: Department of Bioscience and Biotechnology, Shinshu University
– sequence: 14
  givenname: Katsuyoshi
  surname: Hatakeyama
  fullname: Hatakeyama, Katsuyoshi
  organization: Division of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences
BackLink https://cir.nii.ac.jp/crid/1571417124973663872$$DView record in CiNii
https://www.ncbi.nlm.nih.gov/pubmed/21298292$$D View this record in MEDLINE/PubMed
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Keywords Acute abdomen
I-FABP
Biomarker
Intestinal ischemia
ELISA
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PublicationTitle Journal of gastroenterology
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Snippet Background Intestinal fatty acid-binding protein (I-FABP) is a low-molecular-mass (15 kDa) cytosolic protein found exclusively in the epithelial cells of the...
Intestinal fatty acid-binding protein (I-FABP) is a low-molecular-mass (15 kDa) cytosolic protein found exclusively in the epithelial cells of the small bowel...
Background Intestinal fatty acid-binding protein (I-FABP) is a low-molecular-mass (15 kDa) cytosolic protein found exclusively in the epithelial cells of the...
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SubjectTerms Abdomen, Acute - diagnosis
Abdomen, Acute - etiology
Abdominal Surgery
Acute abdomen
Adolescent
Adult
Aged
Aged, 80 and over
Biomarker
Child
Colorectal Surgery
ELISA
Enzyme-Linked Immunosorbent Assay
Enzymes
Fatty Acid-Binding Proteins - blood
Fatty acids
Female
Gastroenterology
Gastrointestinal diseases
Hepatology
Humans
I-FABP
Intestinal Diseases - blood
Intestinal Diseases - diagnosis
Intestinal Diseases - pathology
Intestinal ischemia
Intestine, Small - blood supply
Ischemia
Ischemia - blood
Ischemia - diagnosis
Ischemia - pathology
Male
Measurement
Medicine
Medicine & Public Health
Middle Aged
Monoclonal antibodies
Original Article—Alimentary Tract
Predictive Value of Tests
Protein binding
Sensitivity and Specificity
Single-Blind Method
Surgical Oncology
Young Adult
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Title Diagnosis of ischemic small bowel disease by measurement of serum intestinal fatty acid-binding protein in patients with acute abdomen : a multicenter, observer-blinded validation study
URI https://cir.nii.ac.jp/crid/1571417124973663872
https://link.springer.com/article/10.1007/s00535-011-0373-2
https://www.ncbi.nlm.nih.gov/pubmed/21298292
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https://www.proquest.com/docview/862600269
Volume 46
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