Aging alters gene expression of growth and remodeling factors in human skeletal muscle both at rest and in response to acute resistance exercise

The purpose of this investigation was to compare expression of genes that function in inflammation and stress, cell structure and signaling, or remodeling and growth in skeletal muscle of young (32 +/- 7 yr, n = 15) and elderly (72 +/- 5 yr, n = 16) healthy subjects before and after a bout of resist...

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Vydáno v:	Physiological genomics Ročník 32; číslo 3; s. 393
Hlavní autoři:	Dennis, Richard A, Przybyla, Beata, Gurley, Cathy, Kortebein, Patrick M, Simpson, Pippa, Sullivan, Dennis H, Peterson, Charlotte A
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States 19.02.2008
Témata:	Actins - biosynthesis Actins - genetics Adult Aged Aging - genetics Aging - metabolism Ciliary Neurotrophic Factor - biosynthesis Ciliary Neurotrophic Factor - genetics Gene Expression Profiling Gene Expression Regulation - physiology Humans Insulin-Like Growth Factor Binding Protein 5 - biosynthesis Insulin-Like Growth Factor Binding Protein 5 - genetics Insulin-Like Growth Factor I - biosynthesis Insulin-Like Growth Factor I - genetics Intercellular Signaling Peptides and Proteins - biosynthesis Intercellular Signaling Peptides and Proteins - genetics Male Matrix Metalloproteinase 2 - biosynthesis Matrix Metalloproteinase 2 - genetics Muscle Proteins - biosynthesis Muscle Proteins - genetics Muscle, Skeletal - growth & development Muscle, Skeletal - metabolism Myostatin Rest - physiology Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis RNA, Messenger - genetics Tissue Inhibitor of Metalloproteinase-1 - biosynthesis Tissue Inhibitor of Metalloproteinase-1 - genetics Transforming Growth Factor beta - biosynthesis Transforming Growth Factor beta - genetics Weight Lifting - physiology
ISSN:	1531-2267, 1531-2267
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Shrnutí:	The purpose of this investigation was to compare expression of genes that function in inflammation and stress, cell structure and signaling, or remodeling and growth in skeletal muscle of young (32 +/- 7 yr, n = 15) and elderly (72 +/- 5 yr, n = 16) healthy subjects before and after a bout of resistance leg exercises. A real-time RT-PCR method was used to screen 100 transcripts in v. lateralis biopsies obtained before and 72 h postexercise. The screen identified 15 candidates for differential expression due to aging and/or exercise that were measured quantitatively. The median levels of four mRNAs (insulin-like growth factor-1 and its binding protein IGFBP5, ciliary neurotrophic factor, and the metallopeptidase MMP2) were significantly affected by aging and were greater (1.6- to 2.3-fold, P </= 0.05) in the young than elderly muscle at both time points. The median levels of three mRNAs were significantly (P </= 0.05) affected by exercise in the young. The metallopeptidase inhibitor TIMP1 and alpha-cardiac actin mRNAs increased 2-fold and 6.5-fold, respectively, and GDF8 (myostatin) mRNA decreased by 50%. However, elderly muscle did not display any significant changes in gene expression postexercise. Thus, aging muscle shows decreased levels at rest and an impaired response to exercise for a number of mRNAs for factors potentially involved in muscle growth and remodeling. Future studies must determine the functional importance of these gene expression changes to protein synthesis, satellite cell activity, and other processes that are directly involved in the mechanisms of muscle hypertrophy.
Bibliografie:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	1531-2267 1531-2267
DOI:	10.1152/physiolgenomics.00191.2007