Visualization of Defects on a Cultured Cell Layer by Utilizing Chemical Imaging Sensor
The chemical imaging sensor is a field-effect sensor which is able to visualize both the distribution of ions (in LAPS mode) and the distribution of impedance (in SPIM mode) inthe sample. In this study, a novel wound-healing assay is proposed, in which the chemical imaging sensor operated in SPIM mo...
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| Vydané v: | Procedia engineering Ročník 120; s. 936 - 939 |
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| Hlavní autori: | , , , , |
| Médium: | Journal Article |
| Jazyk: | English |
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Elsevier Ltd
2015
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| ISSN: | 1877-7058, 1877-7058 |
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| Abstract | The chemical imaging sensor is a field-effect sensor which is able to visualize both the distribution of ions (in LAPS mode) and the distribution of impedance (in SPIM mode) inthe sample. In this study, a novel wound-healing assay is proposed, in which the chemical imaging sensor operated in SPIM mode is applied to monitor the defect of a cell layer brought into proximity of the sensing surface.A reduced impedance inside the defect, which was artificially formed ina cell layer, was successfully visualized in a photocurrent image. |
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| AbstractList | The chemical imaging sensor is a field-effect sensor which is able to visualize both the distribution of ions (in LAPS mode) and the distribution of impedance (in SPIM mode) inthe sample. In this study, a novel wound-healing assay is proposed, in which the chemical imaging sensor operated in SPIM mode is applied to monitor the defect of a cell layer brought into proximity of the sensing surface.A reduced impedance inside the defect, which was artificially formed ina cell layer, was successfully visualized in a photocurrent image. |
| Author | Bing, Yu Miyamoto, Ko-ichiro Yoshinobu, Tatsuo Schöning, Michael J. Wagner, Torsten |
| Author_xml | – sequence: 1 givenname: Ko-ichiro surname: Miyamoto fullname: Miyamoto, Ko-ichiro email: k-miya@ecei.tohoku.ac.jp organization: Department of Electronic Engineering, Tohoku University, 6-6-05 Aramaki-aza-Aoba, Aoba-ku, Sendai, 980-8579, Japan – sequence: 2 givenname: Yu surname: Bing fullname: Bing, Yu organization: Department of Electronic Engineering, Tohoku University, 6-6-05 Aramaki-aza-Aoba, Aoba-ku, Sendai, 980-8579, Japan – sequence: 3 givenname: Torsten surname: Wagner fullname: Wagner, Torsten organization: Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, Jülich, 52428, Germany – sequence: 4 givenname: Tatsuo surname: Yoshinobu fullname: Yoshinobu, Tatsuo organization: Department of Electronic Engineering, Tohoku University, 6-6-05 Aramaki-aza-Aoba, Aoba-ku, Sendai, 980-8579, Japan – sequence: 5 givenname: Michael J. surname: Schöning fullname: Schöning, Michael J. organization: Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, Jülich, 52428, Germany |
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| Cites_doi | 10.1016/S0013-4686(02)00088-9 10.1371/journal.pone.0055775 10.1126/science.3375810 10.1016/0925-4005(93)01199-E |
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| References | Hafeman, Parce, McConnell (bib0015) 1988; 27 Krause, Talabani, Xu, Moritz, Griffiths (bib0020) 2002; 47 Nakao, Yoshinobu, Iwasaki (bib0010) 1994; 20 Sumagin, Robin, Nusrat, Parkos (bib0005) 2013; 8 Nakao (10.1016/j.proeng.2015.08.806_bib0010) 1994; 20 Hafeman (10.1016/j.proeng.2015.08.806_bib0015) 1988; 27 Krause (10.1016/j.proeng.2015.08.806_bib0020) 2002; 47 Sumagin (10.1016/j.proeng.2015.08.806_bib0005) 2013; 8 |
| References_xml | – volume: 20 start-page: 119 year: 1994 end-page: 123 ident: bib0010 article-title: Scanning-laser-beam semiconductor pH-imaging sensor publication-title: Sens. Act. B – volume: 8 start-page: e55775 year: 2013 ident: bib0005 article-title: Activation of PKCβII by PMA facilitates enhanced epithelial wound repair through increased cell spreading and migration publication-title: PLoS ONE – volume: 27 start-page: 1182 year: 1988 end-page: 1185 ident: bib0015 article-title: Light-addressable potentiometric sensor for biochemical systems publication-title: Science – volume: 47 start-page: 2143 year: 2002 end-page: 2148 ident: bib0020 article-title: Scanning photo-induced impedance microscopy - an impedance based imaging technique publication-title: Electrochimica Acta – volume: 47 start-page: 2143 year: 2002 ident: 10.1016/j.proeng.2015.08.806_bib0020 article-title: Scanning photo-induced impedance microscopy - an impedance based imaging technique publication-title: Electrochimica Acta doi: 10.1016/S0013-4686(02)00088-9 – volume: 8 start-page: e55775 year: 2013 ident: 10.1016/j.proeng.2015.08.806_bib0005 article-title: Activation of PKCβII by PMA facilitates enhanced epithelial wound repair through increased cell spreading and migration publication-title: PLoS ONE doi: 10.1371/journal.pone.0055775 – volume: 27 start-page: 1182 year: 1988 ident: 10.1016/j.proeng.2015.08.806_bib0015 article-title: Light-addressable potentiometric sensor for biochemical systems publication-title: Science doi: 10.1126/science.3375810 – volume: 20 start-page: 119 year: 1994 ident: 10.1016/j.proeng.2015.08.806_bib0010 article-title: Scanning-laser-beam semiconductor pH-imaging sensor publication-title: Sens. Act. B doi: 10.1016/0925-4005(93)01199-E |
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| Title | Visualization of Defects on a Cultured Cell Layer by Utilizing Chemical Imaging Sensor |
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