Nano-LC–MS/MS for the quantitation of ceramides in mice cerebrospinal fluid using minimal sample volume

A new nano-liquid chromatography–ESI–MS/MS method has been developed for the sensitive quantitation of C8:0, C16:0, C18:0, C18:1, C20:0, C24:1 and C24:0 ceramide in cerebrospinal fluid of mice using minimal sample volume. Volumes of 2µL CSF were undertaken a simple, fast extraction procedure involvi...

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Bibliographic Details
Published in:	Talanta (Oxford) Vol. 116; pp. 912 - 918
Main Authors:	Thomas, D., Eberle, M., Schiffmann, S., Zhang, D.D., Geisslinger, G., Ferreirós, N.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 15.11.2013
Subjects:	Animals Calibration Ceramide ceramides Ceramides - cerebrospinal fluid Ceramides - classification Cerebrospinal fluid Chromatography, Liquid Female Limit of Detection methanol Methanol - chemistry Mice Mice, Inbred C57BL Nano-LC–MS/MS Nanotechnology - instrumentation Nanotechnology - methods Protein Denaturation Reference Standards Reproducibility of Results reversed-phase liquid chromatography Specimen Handling spectrometers Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Ceramide Nano-LC–MS/MS Cerebrospinal fluid
ISSN:	0039-9140, 1873-3573, 1873-3573
Online Access:	Get full text
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