Nano-LC–MS/MS for the quantitation of ceramides in mice cerebrospinal fluid using minimal sample volume

A new nano-liquid chromatography–ESI–MS/MS method has been developed for the sensitive quantitation of C8:0, C16:0, C18:0, C18:1, C20:0, C24:1 and C24:0 ceramide in cerebrospinal fluid of mice using minimal sample volume. Volumes of 2µL CSF were undertaken a simple, fast extraction procedure involvi...

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Vydáno v:	Talanta (Oxford) Ročník 116; s. 912 - 918
Hlavní autoři:	Thomas, D., Eberle, M., Schiffmann, S., Zhang, D.D., Geisslinger, G., Ferreirós, N.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Netherlands Elsevier B.V 15.11.2013
Témata:	Animals Calibration Ceramide ceramides Ceramides - cerebrospinal fluid Ceramides - classification Cerebrospinal fluid Chromatography, Liquid Female Limit of Detection methanol Methanol - chemistry Mice Mice, Inbred C57BL Nano-LC–MS/MS Nanotechnology - instrumentation Nanotechnology - methods Protein Denaturation Reference Standards Reproducibility of Results reversed-phase liquid chromatography Specimen Handling spectrometers Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Ceramide Nano-LC–MS/MS Cerebrospinal fluid
ISSN:	0039-9140, 1873-3573, 1873-3573
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Abstract	A new nano-liquid chromatography–ESI–MS/MS method has been developed for the sensitive quantitation of C8:0, C16:0, C18:0, C18:1, C20:0, C24:1 and C24:0 ceramide in cerebrospinal fluid of mice using minimal sample volume. Volumes of 2µL CSF were undertaken a simple, fast extraction procedure involving protein precipitation with methanol and dilution. Ceramides were separated by nano-liquid chromatography with a reversed phase C8 column and detected with a triple quadrupole mass spectrometer. C17:0 ceramide was used as internal standard. The method has been validated in terms of linearity, lower limit of quantitation, precision, accuracy and autosampler stability. Calibration curves covered a range of 2.25–120pg/µL for most ceramides (7.5–120pg/µL for C24:0 ceramide). The lower limits of quantitation determined for C8:0, C16:0, C18:1, C18:0, C20:0 and C24:1 ceramide were 0.225pg on column (2.25pg/µL) and that for C24:0 ceramide 0.750pg on column (7.5pg/µL). Intra- and interday precision and accuracy values, expressed as relative standard deviation and relative error, respectively, were lower than 15% in all cases. Autosampler stability for calibration standards and CSF samples was proven for at least 24h for all analytes. The suitability of the method has been demonstrated by quantifying the analytes, except the non-endogenous C8:0 ceramide, in cerebrospinal fluid samples of 12 mice. Calculated concentrations ranged from 3 to 120pg/µL in cerebrospinal fluid for all analytes, except for C24:0 ceramide, which could not be detected in any of the analyzed samples. •A very high sensitive method for ceramides in cerebrospinal fluid was developed.•Only 2µL sample volume was required.•Validation according to the FDA guidelines was performed.•First approach using nano-LC–MS/MS for quantitation of ceramides.
AbstractList	A new nano-liquid chromatography–ESI–MS/MS method has been developed for the sensitive quantitation of C8:0, C16:0, C18:0, C18:1, C20:0, C24:1 and C24:0 ceramide in cerebrospinal fluid of mice using minimal sample volume. Volumes of 2µL CSF were undertaken a simple, fast extraction procedure involving protein precipitation with methanol and dilution. Ceramides were separated by nano-liquid chromatography with a reversed phase C8 column and detected with a triple quadrupole mass spectrometer. C17:0 ceramide was used as internal standard. The method has been validated in terms of linearity, lower limit of quantitation, precision, accuracy and autosampler stability. Calibration curves covered a range of 2.25–120pg/µL for most ceramides (7.5–120pg/µL for C24:0 ceramide). The lower limits of quantitation determined for C8:0, C16:0, C18:1, C18:0, C20:0 and C24:1 ceramide were 0.225pg on column (2.25pg/µL) and that for C24:0 ceramide 0.750pg on column (7.5pg/µL). Intra- and interday precision and accuracy values, expressed as relative standard deviation and relative error, respectively, were lower than 15% in all cases. Autosampler stability for calibration standards and CSF samples was proven for at least 24h for all analytes. The suitability of the method has been demonstrated by quantifying the analytes, except the non-endogenous C8:0 ceramide, in cerebrospinal fluid samples of 12 mice. Calculated concentrations ranged from 3 to 120pg/µL in cerebrospinal fluid for all analytes, except for C24:0 ceramide, which could not be detected in any of the analyzed samples. A new nano-liquid chromatography-ESI-MS/MS method has been developed for the sensitive quantitation of C8:0, C16:0, C18:0, C18:1, C20:0, C24:1 and C24:0 ceramide in cerebrospinal fluid of mice using minimal sample volume. Volumes of 2 µL CSF were undertaken a simple, fast extraction procedure involving protein precipitation with methanol and dilution. Ceramides were separated by nano-liquid chromatography with a reversed phase C8 column and detected with a triple quadrupole mass spectrometer. C17:0 ceramide was used as internal standard. The method has been validated in terms of linearity, lower limit of quantitation, precision, accuracy and autosampler stability. Calibration curves covered a range of 2.25-120 pg/µL for most ceramides (7.5-120 pg/µL for C24:0 ceramide). The lower limits of quantitation determined for C8:0, C16:0, C18:1, C18:0, C20:0 and C24:1 ceramide were 0.225 pg on column (2.25 pg/µL) and that for C24:0 ceramide 0.750 pg on column (7.5 pg/µL). Intra- and interday precision and accuracy values, expressed as relative standard deviation and relative error, respectively, were lower than 15% in all cases. Autosampler stability for calibration standards and CSF samples was proven for at least 24h for all analytes. The suitability of the method has been demonstrated by quantifying the analytes, except the non-endogenous C8:0 ceramide, in cerebrospinal fluid samples of 12 mice. Calculated concentrations ranged from 3 to 120 pg/µL in cerebrospinal fluid for all analytes, except for C24:0 ceramide, which could not be detected in any of the analyzed samples.A new nano-liquid chromatography-ESI-MS/MS method has been developed for the sensitive quantitation of C8:0, C16:0, C18:0, C18:1, C20:0, C24:1 and C24:0 ceramide in cerebrospinal fluid of mice using minimal sample volume. Volumes of 2 µL CSF were undertaken a simple, fast extraction procedure involving protein precipitation with methanol and dilution. Ceramides were separated by nano-liquid chromatography with a reversed phase C8 column and detected with a triple quadrupole mass spectrometer. C17:0 ceramide was used as internal standard. The method has been validated in terms of linearity, lower limit of quantitation, precision, accuracy and autosampler stability. Calibration curves covered a range of 2.25-120 pg/µL for most ceramides (7.5-120 pg/µL for C24:0 ceramide). The lower limits of quantitation determined for C8:0, C16:0, C18:1, C18:0, C20:0 and C24:1 ceramide were 0.225 pg on column (2.25 pg/µL) and that for C24:0 ceramide 0.750 pg on column (7.5 pg/µL). Intra- and interday precision and accuracy values, expressed as relative standard deviation and relative error, respectively, were lower than 15% in all cases. Autosampler stability for calibration standards and CSF samples was proven for at least 24h for all analytes. The suitability of the method has been demonstrated by quantifying the analytes, except the non-endogenous C8:0 ceramide, in cerebrospinal fluid samples of 12 mice. Calculated concentrations ranged from 3 to 120 pg/µL in cerebrospinal fluid for all analytes, except for C24:0 ceramide, which could not be detected in any of the analyzed samples. A new nano-liquid chromatography–ESI–MS/MS method has been developed for the sensitive quantitation of C8:0, C16:0, C18:0, C18:1, C20:0, C24:1 and C24:0 ceramide in cerebrospinal fluid of mice using minimal sample volume. Volumes of 2µL CSF were undertaken a simple, fast extraction procedure involving protein precipitation with methanol and dilution. Ceramides were separated by nano-liquid chromatography with a reversed phase C8 column and detected with a triple quadrupole mass spectrometer. C17:0 ceramide was used as internal standard. The method has been validated in terms of linearity, lower limit of quantitation, precision, accuracy and autosampler stability. Calibration curves covered a range of 2.25–120pg/µL for most ceramides (7.5–120pg/µL for C24:0 ceramide). The lower limits of quantitation determined for C8:0, C16:0, C18:1, C18:0, C20:0 and C24:1 ceramide were 0.225pg on column (2.25pg/µL) and that for C24:0 ceramide 0.750pg on column (7.5pg/µL). Intra- and interday precision and accuracy values, expressed as relative standard deviation and relative error, respectively, were lower than 15% in all cases. Autosampler stability for calibration standards and CSF samples was proven for at least 24h for all analytes. The suitability of the method has been demonstrated by quantifying the analytes, except the non-endogenous C8:0 ceramide, in cerebrospinal fluid samples of 12 mice. Calculated concentrations ranged from 3 to 120pg/µL in cerebrospinal fluid for all analytes, except for C24:0 ceramide, which could not be detected in any of the analyzed samples. •A very high sensitive method for ceramides in cerebrospinal fluid was developed.•Only 2µL sample volume was required.•Validation according to the FDA guidelines was performed.•First approach using nano-LC–MS/MS for quantitation of ceramides. A new nano-liquid chromatography-ESI-MS/MS method has been developed for the sensitive quantitation of C8:0, C16:0, C18:0, C18:1, C20:0, C24:1 and C24:0 ceramide in cerebrospinal fluid of mice using minimal sample volume. Volumes of 2 µL CSF were undertaken a simple, fast extraction procedure involving protein precipitation with methanol and dilution. Ceramides were separated by nano-liquid chromatography with a reversed phase C8 column and detected with a triple quadrupole mass spectrometer. C17:0 ceramide was used as internal standard. The method has been validated in terms of linearity, lower limit of quantitation, precision, accuracy and autosampler stability. Calibration curves covered a range of 2.25-120 pg/µL for most ceramides (7.5-120 pg/µL for C24:0 ceramide). The lower limits of quantitation determined for C8:0, C16:0, C18:1, C18:0, C20:0 and C24:1 ceramide were 0.225 pg on column (2.25 pg/µL) and that for C24:0 ceramide 0.750 pg on column (7.5 pg/µL). Intra- and interday precision and accuracy values, expressed as relative standard deviation and relative error, respectively, were lower than 15% in all cases. Autosampler stability for calibration standards and CSF samples was proven for at least 24h for all analytes. The suitability of the method has been demonstrated by quantifying the analytes, except the non-endogenous C8:0 ceramide, in cerebrospinal fluid samples of 12 mice. Calculated concentrations ranged from 3 to 120 pg/µL in cerebrospinal fluid for all analytes, except for C24:0 ceramide, which could not be detected in any of the analyzed samples.
Author	Zhang, D.D. Ferreirós, N. Eberle, M. Schiffmann, S. Thomas, D. Geisslinger, G.
Author_xml	– sequence: 1 givenname: D. surname: Thomas fullname: Thomas, D. – sequence: 2 givenname: M. surname: Eberle fullname: Eberle, M. – sequence: 3 givenname: S. surname: Schiffmann fullname: Schiffmann, S. – sequence: 4 givenname: D.D. surname: Zhang fullname: Zhang, D.D. – sequence: 5 givenname: G. surname: Geisslinger fullname: Geisslinger, G. – sequence: 6 givenname: N. surname: Ferreirós fullname: Ferreirós, N. email: ferreirosbouzas@em.uni-frankfurt.de
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Cites_doi	10.1016/j.ab.2010.02.009 10.1002/jssc.200700061 10.2337/db06-0330 10.2337/db08-1228 10.1016/j.chroma.2009.05.080 10.1002/jssc.200500142 10.1007/s12017-010-8132-8 10.1016/j.jchromb.2009.01.003 10.1074/jbc.R200008200 10.1016/j.ymeth.2006.05.004 10.1093/clinchem/30.2.290 10.1073/pnas.0305799101 10.1002/jssc.200301712 10.1016/j.talanta.2009.07.035 10.1016/S0039-9140(01)00565-3 10.1038/nrc1411 10.1016/j.ab.2010.02.023 10.1007/BF01466734 10.1007/s10549-011-1768-8 10.2174/157341209789649159 10.1016/j.neuroscience.2004.08.056 10.1002/rcm.1692 10.1016/j.bbalip.2011.05.011 10.4049/jimmunol.1103109 10.1194/jlr.D800051-JLR200 10.1016/j.atherosclerosis.2007.09.018 10.1016/j.jchromb.2009.07.008 10.1016/j.ab.2008.03.045 10.1016/j.jchromb.2006.06.025 10.1016/j.plipres.2011.11.001 10.1016/j.jchromb.2007.02.030 10.1016/S0003-2670(99)00661-3 10.1021/ac00168a006 10.1016/j.talanta.2008.09.003 10.1016/j.ab.2012.01.027 10.1016/j.chemphyslip.2011.04.011 10.1016/j.ymeth.2005.01.009 10.1038/nm1748 10.1016/j.ab.2008.12.010 10.1016/j.brainres.2007.10.066
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References	Kasumov, Huang, Chung, Zhang, McCullough, Kirwan (bib20) 2010; 401 Teichgräber, Ulrich, Endlich, Riethmüller, Wilker, De Oliveira–Munding, van Heeckeren, Barr, von Kürthy, Schmid, Weller, Tümmler, Lang, Grassme, Döring, Gulbins (bib12) 2008; 14 Hu, Deng, Liu, Zhang (bib37) 2009; 77 Satoi, Tomimoto, Ohtani, Kitano, Kondo, Watanabe, Oka, Akiguchi, Furuya, Hirabayashi, Okazaki (bib7) 2005; 130 pdf, accessed 04/04/2013, (2001). Samad (bib5) 2006; 55 Haynes, Duerksen-Hughes, Filippova, Filippov, Zhang (bib21) 2008; 378 Bielawski, Szulc, Hannun, Bielawska (bib22) 2006; 39 Bui, Leohr, Kuo (bib26) 2012; 423 FDA, Center for Drug Evaluation and Research, Rockville, MD, USA Haus, Kashyap, Kasumov, Zhang, Kelly, DeFronzo, Kirwan (bib4) 2008; 58 Liou, Sheu, Liu, Lin, Ho (bib18) 2010; 401 Van Eeckhaut, Lanckmans, Sarre, Smolders, Michotte (bib42) 2009; 877 Ravanat, Wurtz, Ohlmann, Fichter, Cazenave, VanDorsselaer, Lanza, Gachet (bib31) 2009; 386 Murielle Mimeault (bib3) 2002; 56 Raith, Neubert (bib24) 2000; 403 Farwanah, Pierstorff, Schmelzer, Raith, Neubert, Kolter, Sandhoff (bib2) 2007; 852 Song, Quan, Liu (bib35) 2004; 18 Yoo, Son, Kim (bib23) 2006; 843 Felgenhauer (bib41) 1974; 52 Schiffmann, Ferreiros, Birod, Eberle, Schreiber, Pfeilschifter, Ziemann, Pierre, Scholich, Grosch, Geisslinger (bib13) 2012; 188 Camera, Picardo, Presutti, Catarcini, Fanali (bib19) 2004; 27 Hernández-Borges, Aturki, Rocco, Fanali (bib28) 2007; 30 Hannun (bib1) 2002; 277 Liu, Cobb, Johnson, Wang, Agar (bib32) 2013 Chen, Liu, Sullards, Merrill (bib15) 2010; 12 Ogretmen, Hannun (bib10) 2004; 4 Aboul-Enein (bib29) 2009; 5 Merrill, Sullards, Allegood, Kelly, Wang (bib38) 2005; 36 Bismuth, Lin, Yao, Chen (bib6) 2008; 196 Hsieh, Huang, Lee (bib33) 2009; 1216 Grösch, Schiffmann, Geisslinger (bib11) 2012; 51 Shaner, Allegood, Park, Wang, Kelly, Haynes, Sullards, Merrill (bib25) 2009; 50 Fanali, Aturki, Kasicka, Raggi, D'Orazio (bib34) 2005; 28 Flowers, Fabriás, Delgado, Casas, Abad, Cabot (bib14) 2011; 133 Karu, Turton, Wang, Griffiths (bib36) 2011; 164 Jan, Krouwer (bib40) 1984; 30 Karlsson, Novotny (bib27) 1988; 60 Marks, Berg, Saito, Saito (bib8) 2008; 1191 Farwanah, Kolter, Sandhoff (bib16) 2011; 1811 Farwanah, Wirtz, Kolter, Raith, Neubert, Sandhoff (bib17) 2009; 877 Turtoi, Mazzucchelli, De Pauw (bib30) 2010; 80 R.G. Cutler, in: Proceedings of the National Academy of Sciences, 101, 2004, pp. 2070–2075. Song (10.1016/j.talanta.2013.07.057_bib35) 2004; 18 Hu (10.1016/j.talanta.2013.07.057_bib37) 2009; 77 Chen (10.1016/j.talanta.2013.07.057_bib15) 2010; 12 Hsieh (10.1016/j.talanta.2013.07.057_bib33) 2009; 1216 Bui (10.1016/j.talanta.2013.07.057_bib26) 2012; 423 Farwanah (10.1016/j.talanta.2013.07.057_bib2) 2007; 852 Ravanat (10.1016/j.talanta.2013.07.057_bib31) 2009; 386 Ogretmen (10.1016/j.talanta.2013.07.057_bib10) 2004; 4 Liou (10.1016/j.talanta.2013.07.057_bib18) 2010; 401 Camera (10.1016/j.talanta.2013.07.057_bib19) 2004; 27 Hernández-Borges (10.1016/j.talanta.2013.07.057_bib28) 2007; 30 Liu (10.1016/j.talanta.2013.07.057_bib32) 2013 Haynes (10.1016/j.talanta.2013.07.057_bib21) 2008; 378 Samad (10.1016/j.talanta.2013.07.057_bib5) 2006; 55 Karlsson (10.1016/j.talanta.2013.07.057_bib27) 1988; 60 Farwanah (10.1016/j.talanta.2013.07.057_bib17) 2009; 877 Bismuth (10.1016/j.talanta.2013.07.057_bib6) 2008; 196 Farwanah (10.1016/j.talanta.2013.07.057_bib16) 2011; 1811 Haus (10.1016/j.talanta.2013.07.057_bib4) 2008; 58 Teichgräber (10.1016/j.talanta.2013.07.057_bib12) 2008; 14 Grösch (10.1016/j.talanta.2013.07.057_bib11) 2012; 51 Schiffmann (10.1016/j.talanta.2013.07.057_bib13) 2012; 188 Hannun (10.1016/j.talanta.2013.07.057_bib1) 2002; 277 Flowers (10.1016/j.talanta.2013.07.057_bib14) 2011; 133 Satoi (10.1016/j.talanta.2013.07.057_bib7) 2005; 130 Marks (10.1016/j.talanta.2013.07.057_bib8) 2008; 1191 Yoo (10.1016/j.talanta.2013.07.057_bib23) 2006; 843 Kasumov (10.1016/j.talanta.2013.07.057_bib20) 2010; 401 Aboul-Enein (10.1016/j.talanta.2013.07.057_bib29) 2009; 5 Felgenhauer (10.1016/j.talanta.2013.07.057_bib41) 1974; 52 10.1016/j.talanta.2013.07.057_bib39 10.1016/j.talanta.2013.07.057_bib9 Merrill (10.1016/j.talanta.2013.07.057_bib38) 2005; 36 Bielawski (10.1016/j.talanta.2013.07.057_bib22) 2006; 39 Fanali (10.1016/j.talanta.2013.07.057_bib34) 2005; 28 Murielle Mimeault (10.1016/j.talanta.2013.07.057_bib3) 2002; 56 Jan (10.1016/j.talanta.2013.07.057_bib40) 1984; 30 Raith (10.1016/j.talanta.2013.07.057_bib24) 2000; 403 Van Eeckhaut (10.1016/j.talanta.2013.07.057_bib42) 2009; 877 Karu (10.1016/j.talanta.2013.07.057_bib36) 2011; 164 Shaner (10.1016/j.talanta.2013.07.057_bib25) 2009; 50 Turtoi (10.1016/j.talanta.2013.07.057_bib30) 2010; 80
References_xml	– volume: 14 start-page: 382 year: 2008 end-page: 391 ident: bib12 publication-title: Nat. Med. – volume: 877 start-page: 2976 year: 2009 end-page: 2982 ident: bib17 publication-title: J. Chromatogr. B – volume: 27 start-page: 971 year: 2004 end-page: 976 ident: bib19 publication-title: J. Sep. Sci. – volume: 843 start-page: 327 year: 2006 end-page: 333 ident: bib23 publication-title: J. Chromatogr. B – volume: 5 start-page: 367 year: 2009 end-page: 380 ident: bib29 publication-title: Curr. Pharm. Anal. – volume: 877 start-page: 2198 year: 2009 end-page: 2207 ident: bib42 publication-title: J. Chromatogr. B – volume: 80 start-page: 1487 year: 2010 end-page: 1495 ident: bib30 publication-title: Talanta – volume: 196 start-page: 497 year: 2008 end-page: 504 ident: bib6 publication-title: Atherosclerosis – volume: 39 start-page: 82 year: 2006 end-page: 91 ident: bib22 publication-title: Methods – year: 2013 ident: bib32 publication-title: J. Chromatogr. Sci. – volume: 386 start-page: 237 year: 2009 end-page: 243 ident: bib31 publication-title: Anal. Biochem. – volume: 401 start-page: 107 year: 2010 end-page: 113 ident: bib18 publication-title: Anal. Biochem. – reference: R.G. Cutler, in: Proceedings of the National Academy of Sciences, 101, 2004, pp. 2070–2075. – volume: 30 start-page: 1589 year: 2007 end-page: 1610 ident: bib28 publication-title: J. Sep. Sci. – volume: 36 start-page: 207 year: 2005 end-page: 224 ident: bib38 publication-title: Methods – volume: 277 start-page: 25847 year: 2002 end-page: 25850 ident: bib1 publication-title: J. Biol. Chem. – volume: 55 start-page: 2579 year: 2006 end-page: 2587 ident: bib5 publication-title: Diabetes – volume: 133 start-page: 447 year: 2011 end-page: 458 ident: bib14 publication-title: Breast Cancer Res. Treat. – volume: 1811 start-page: 854 year: 2011 end-page: 860 ident: bib16 publication-title: Biochim. Biophys. Acta (BBA) – Mol. Cell Biol. Lipids – volume: 1216 start-page: 7186 year: 2009 end-page: 7194 ident: bib33 publication-title: J. Chromatogr. A. – reference: .pdf, accessed 04/04/2013, (2001). – volume: 4 start-page: 604 year: 2004 end-page: 616 ident: bib10 publication-title: Nat. Rev. Cancer – volume: 403 start-page: 295 year: 2000 end-page: 303 ident: bib24 publication-title: Anal. Chim. Acta – volume: 18 start-page: 2818 year: 2004 end-page: 2822 ident: bib35 publication-title: Rapid Commun. Mass Spectrom. – volume: 56 start-page: 395 year: 2002 end-page: 405 ident: bib3 publication-title: Talanta – volume: 58 start-page: 337 year: 2008 end-page: 343 ident: bib4 publication-title: Diabetes – volume: 51 start-page: 50 year: 2012 end-page: 62 ident: bib11 publication-title: Prog. Lipid Res. – volume: 423 start-page: 187 year: 2012 end-page: 194 ident: bib26 publication-title: Anal. Biochem. – volume: 401 start-page: 154 year: 2010 end-page: 161 ident: bib20 publication-title: Anal. Biochem. – volume: 50 start-page: 1692 year: 2009 end-page: 1707 ident: bib25 publication-title: J. Lipid Res. – volume: 378 start-page: 80 year: 2008 end-page: 86 ident: bib21 publication-title: Anal. Biochem. – volume: 30 start-page: 290 year: 1984 end-page: 292 ident: bib40 publication-title: Clin. Chem. – volume: 52 start-page: 1158 year: 1974 end-page: 1164 ident: bib41 publication-title: Klin. Wochenschr. – volume: 852 start-page: 562 year: 2007 end-page: 570 ident: bib2 publication-title: J.Chromatogr. B – volume: 12 start-page: 306 year: 2010 end-page: 319 ident: bib15 publication-title: Neuromol. Med. – volume: 130 start-page: 657 year: 2005 end-page: 666 ident: bib7 publication-title: Neuroscience – volume: 188 start-page: 5723 year: 2012 end-page: 5733 ident: bib13 publication-title: J. Immunol. – volume: 60 start-page: 1662 year: 1988 end-page: 1665 ident: bib27 publication-title: Anal. Chem. – volume: 164 start-page: 411 year: 2011 end-page: 424 ident: bib36 publication-title: Chem. Phys. Lipids – volume: 77 start-page: 1299 year: 2009 end-page: 1303 ident: bib37 publication-title: Talanta – reference: FDA, Center for Drug Evaluation and Research, Rockville, MD, USA, – volume: 1191 start-page: 136 year: 2008 end-page: 147 ident: bib8 publication-title: Brain Res. – volume: 28 start-page: 1719 year: 2005 end-page: 1728 ident: bib34 publication-title: J. Sep. Sci. – volume: 401 start-page: 107 year: 2010 ident: 10.1016/j.talanta.2013.07.057_bib18 publication-title: Anal. Biochem. doi: 10.1016/j.ab.2010.02.009 – volume: 30 start-page: 1589 year: 2007 ident: 10.1016/j.talanta.2013.07.057_bib28 publication-title: J. Sep. Sci. doi: 10.1002/jssc.200700061 – volume: 55 start-page: 2579 year: 2006 ident: 10.1016/j.talanta.2013.07.057_bib5 publication-title: Diabetes doi: 10.2337/db06-0330 – year: 2013 ident: 10.1016/j.talanta.2013.07.057_bib32 publication-title: J. Chromatogr. Sci. – volume: 58 start-page: 337 year: 2008 ident: 10.1016/j.talanta.2013.07.057_bib4 publication-title: Diabetes doi: 10.2337/db08-1228 – volume: 1216 start-page: 7186 year: 2009 ident: 10.1016/j.talanta.2013.07.057_bib33 publication-title: J. Chromatogr. A. doi: 10.1016/j.chroma.2009.05.080 – volume: 28 start-page: 1719 year: 2005 ident: 10.1016/j.talanta.2013.07.057_bib34 publication-title: J. Sep. Sci. doi: 10.1002/jssc.200500142 – volume: 12 start-page: 306 year: 2010 ident: 10.1016/j.talanta.2013.07.057_bib15 publication-title: Neuromol. Med. doi: 10.1007/s12017-010-8132-8 – volume: 877 start-page: 2198 year: 2009 ident: 10.1016/j.talanta.2013.07.057_bib42 publication-title: J. Chromatogr. B doi: 10.1016/j.jchromb.2009.01.003 – volume: 277 start-page: 25847 year: 2002 ident: 10.1016/j.talanta.2013.07.057_bib1 publication-title: J. Biol. Chem. doi: 10.1074/jbc.R200008200 – volume: 39 start-page: 82 year: 2006 ident: 10.1016/j.talanta.2013.07.057_bib22 publication-title: Methods doi: 10.1016/j.ymeth.2006.05.004 – ident: 10.1016/j.talanta.2013.07.057_bib39 – volume: 30 start-page: 290 year: 1984 ident: 10.1016/j.talanta.2013.07.057_bib40 publication-title: Clin. Chem. doi: 10.1093/clinchem/30.2.290 – ident: 10.1016/j.talanta.2013.07.057_bib9 doi: 10.1073/pnas.0305799101 – volume: 27 start-page: 971 year: 2004 ident: 10.1016/j.talanta.2013.07.057_bib19 publication-title: J. Sep. Sci. doi: 10.1002/jssc.200301712 – volume: 80 start-page: 1487 year: 2010 ident: 10.1016/j.talanta.2013.07.057_bib30 publication-title: Talanta doi: 10.1016/j.talanta.2009.07.035 – volume: 56 start-page: 395 year: 2002 ident: 10.1016/j.talanta.2013.07.057_bib3 publication-title: Talanta doi: 10.1016/S0039-9140(01)00565-3 – volume: 4 start-page: 604 year: 2004 ident: 10.1016/j.talanta.2013.07.057_bib10 publication-title: Nat. Rev. Cancer doi: 10.1038/nrc1411 – volume: 401 start-page: 154 year: 2010 ident: 10.1016/j.talanta.2013.07.057_bib20 publication-title: Anal. Biochem. doi: 10.1016/j.ab.2010.02.023 – volume: 52 start-page: 1158 year: 1974 ident: 10.1016/j.talanta.2013.07.057_bib41 publication-title: Klin. Wochenschr. doi: 10.1007/BF01466734 – volume: 133 start-page: 447 year: 2011 ident: 10.1016/j.talanta.2013.07.057_bib14 publication-title: Breast Cancer Res. Treat. doi: 10.1007/s10549-011-1768-8 – volume: 5 start-page: 367 year: 2009 ident: 10.1016/j.talanta.2013.07.057_bib29 publication-title: Curr. Pharm. Anal. doi: 10.2174/157341209789649159 – volume: 130 start-page: 657 year: 2005 ident: 10.1016/j.talanta.2013.07.057_bib7 publication-title: Neuroscience doi: 10.1016/j.neuroscience.2004.08.056 – volume: 18 start-page: 2818 year: 2004 ident: 10.1016/j.talanta.2013.07.057_bib35 publication-title: Rapid Commun. Mass Spectrom. doi: 10.1002/rcm.1692 – volume: 1811 start-page: 854 year: 2011 ident: 10.1016/j.talanta.2013.07.057_bib16 publication-title: Biochim. Biophys. Acta (BBA) – Mol. Cell Biol. Lipids doi: 10.1016/j.bbalip.2011.05.011 – volume: 188 start-page: 5723 year: 2012 ident: 10.1016/j.talanta.2013.07.057_bib13 publication-title: J. Immunol. doi: 10.4049/jimmunol.1103109 – volume: 50 start-page: 1692 year: 2009 ident: 10.1016/j.talanta.2013.07.057_bib25 publication-title: J. Lipid Res. doi: 10.1194/jlr.D800051-JLR200 – volume: 196 start-page: 497 year: 2008 ident: 10.1016/j.talanta.2013.07.057_bib6 publication-title: Atherosclerosis doi: 10.1016/j.atherosclerosis.2007.09.018 – volume: 877 start-page: 2976 year: 2009 ident: 10.1016/j.talanta.2013.07.057_bib17 publication-title: J. Chromatogr. B doi: 10.1016/j.jchromb.2009.07.008 – volume: 378 start-page: 80 year: 2008 ident: 10.1016/j.talanta.2013.07.057_bib21 publication-title: Anal. Biochem. doi: 10.1016/j.ab.2008.03.045 – volume: 843 start-page: 327 year: 2006 ident: 10.1016/j.talanta.2013.07.057_bib23 publication-title: J. Chromatogr. B doi: 10.1016/j.jchromb.2006.06.025 – volume: 51 start-page: 50 year: 2012 ident: 10.1016/j.talanta.2013.07.057_bib11 publication-title: Prog. Lipid Res. doi: 10.1016/j.plipres.2011.11.001 – volume: 852 start-page: 562 year: 2007 ident: 10.1016/j.talanta.2013.07.057_bib2 publication-title: J.Chromatogr. B doi: 10.1016/j.jchromb.2007.02.030 – volume: 403 start-page: 295 year: 2000 ident: 10.1016/j.talanta.2013.07.057_bib24 publication-title: Anal. Chim. Acta doi: 10.1016/S0003-2670(99)00661-3 – volume: 60 start-page: 1662 year: 1988 ident: 10.1016/j.talanta.2013.07.057_bib27 publication-title: Anal. Chem. doi: 10.1021/ac00168a006 – volume: 77 start-page: 1299 year: 2009 ident: 10.1016/j.talanta.2013.07.057_bib37 publication-title: Talanta doi: 10.1016/j.talanta.2008.09.003 – volume: 423 start-page: 187 year: 2012 ident: 10.1016/j.talanta.2013.07.057_bib26 publication-title: Anal. Biochem. doi: 10.1016/j.ab.2012.01.027 – volume: 164 start-page: 411 year: 2011 ident: 10.1016/j.talanta.2013.07.057_bib36 publication-title: Chem. Phys. Lipids doi: 10.1016/j.chemphyslip.2011.04.011 – volume: 36 start-page: 207 year: 2005 ident: 10.1016/j.talanta.2013.07.057_bib38 publication-title: Methods doi: 10.1016/j.ymeth.2005.01.009 – volume: 14 start-page: 382 year: 2008 ident: 10.1016/j.talanta.2013.07.057_bib12 publication-title: Nat. Med. doi: 10.1038/nm1748 – volume: 386 start-page: 237 year: 2009 ident: 10.1016/j.talanta.2013.07.057_bib31 publication-title: Anal. Biochem. doi: 10.1016/j.ab.2008.12.010 – volume: 1191 start-page: 136 year: 2008 ident: 10.1016/j.talanta.2013.07.057_bib8 publication-title: Brain Res. doi: 10.1016/j.brainres.2007.10.066
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Snippet	A new nano-liquid chromatography–ESI–MS/MS method has been developed for the sensitive quantitation of C8:0, C16:0, C18:0, C18:1, C20:0, C24:1 and C24:0... A new nano-liquid chromatography-ESI-MS/MS method has been developed for the sensitive quantitation of C8:0, C16:0, C18:0, C18:1, C20:0, C24:1 and C24:0...
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SubjectTerms	Animals Calibration Ceramide ceramides Ceramides - cerebrospinal fluid Ceramides - classification Cerebrospinal fluid Chromatography, Liquid Female Limit of Detection methanol Methanol - chemistry Mice Mice, Inbred C57BL Nano-LC–MS/MS Nanotechnology - instrumentation Nanotechnology - methods Protein Denaturation Reference Standards Reproducibility of Results reversed-phase liquid chromatography Specimen Handling spectrometers Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry
Title	Nano-LC–MS/MS for the quantitation of ceramides in mice cerebrospinal fluid using minimal sample volume
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