COVID-19 immune features revealed by a large-scale single-cell transcriptome atlas

A dysfunctional immune response in coronavirus disease 2019 (COVID-19) patients is a recurrent theme impacting symptoms and mortality, yet a detailed understanding of pertinent immune cells is not complete. We applied single-cell RNA sequencing to 284 samples from 196 COVID-19 patients and controls...

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Published in:Cell Vol. 184; no. 7; p. 1895
Main Authors: Ren, Xianwen, Wen, Wen, Fan, Xiaoying, Hou, Wenhong, Su, Bin, Cai, Pengfei, Li, Jiesheng, Liu, Yang, Tang, Fei, Zhang, Fan, Yang, Yu, He, Jiangping, Ma, Wenji, He, Jingjing, Wang, Pingping, Cao, Qiqi, Chen, Fangjin, Chen, Yuqing, Cheng, Xuelian, Deng, Guohong, Deng, Xilong, Ding, Wenyu, Feng, Yingmei, Gan, Rui, Guo, Chuang, Guo, Weiqiang, He, Shuai, Jiang, Chen, Liang, Juanran, Li, Yi-Min, Lin, Jun, Ling, Yun, Liu, Haofei, Liu, Jianwei, Liu, Nianping, Liu, Shu-Qiang, Luo, Meng, Ma, Qiang, Song, Qibing, Sun, Wujianan, Wang, GaoXiang, Wang, Feng, Wang, Ying, Wen, Xiaofeng, Wu, Qian, Xu, Gang, Xie, Xiaowei, Xiong, Xinxin, Xing, Xudong, Xu, Hao, Yin, Chonghai, Yu, Dongdong, Yu, Kezhuo, Yuan, Jin, Zhang, Biao, Zhang, Peipei, Zhang, Tong, Zhao, Jincun, Zhao, Peidong, Zhou, Jianfeng, Zhou, Wei, Zhong, Sujuan, Zhong, Xiaosong, Zhang, Shuye, Zhu, Lin, Zhu, Ping, Zou, Bin, Zou, Jiahua, Zuo, Zengtao, Bai, Fan, Huang, Xi, Zhou, Penghui, Jiang, Qinghua, Huang, Zhiwei, Bei, Jin-Xin, Wei, Lai, Bian, Xiu-Wu, Liu, Xindong, Cheng, Tao, Li, Xiangpan, Zhao, Pingsen, Wang, Fu-Sheng, Wang, Hongyang, Su, Bing, Zhang, Zheng, Qu, Kun, Wang, Xiaoqun, Chen, Jiekai, Jin, Ronghua, Zhang, Zemin
Format: Journal Article
Language:English
Published: United States 01.04.2021
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Child
China
Cohort Studies
COVID-19 - immunology
Cytokines - metabolism
Female
Humans
Male
Megakaryocytes - immunology
Middle Aged
Monocytes - immunology
RNA, Viral - blood
RNA, Viral - isolation & purification
SARS-CoV-2 - genetics
Single-Cell Analysis
Transcriptome - immunology
Young Adult
China
COVID-19
SARS-CoV-2
single-cell transcriptomics
cytokine storm
host cell range
B cell receptor sequencing
cell-cell interaction
single-cell RNA-seq
ligand-receptor interaction
T cell receptor sequencing
ISSN:1097-4172, 1097-4172
Online Access:Get more information
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Summary:A dysfunctional immune response in coronavirus disease 2019 (COVID-19) patients is a recurrent theme impacting symptoms and mortality, yet a detailed understanding of pertinent immune cells is not complete. We applied single-cell RNA sequencing to 284 samples from 196 COVID-19 patients and controls and created a comprehensive immune landscape with 1.46 million cells. The large dataset enabled us to identify that different peripheral immune subtype changes are associated with distinct clinical features, including age, sex, severity, and disease stages of COVID-19. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA was found in diverse epithelial and immune cell types, accompanied by dramatic transcriptomic changes within virus-positive cells. Systemic upregulation of S100A8/A9, mainly by megakaryocytes and monocytes in the peripheral blood, may contribute to the cytokine storms frequently observed in severe patients. Our data provide a rich resource for understanding the pathogenesis of and developing effective therapeutic strategies for COVID-19.
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content type line 23
ISSN:1097-4172
1097-4172
DOI:10.1016/j.cell.2021.01.053