Molecular analysis of the luminal- and mucosal-associated intestinal microbiota in diarrhea-predominant irritable bowel syndrome

Alterations in the intestinal microbiota have been suggested as an etiological factor in the pathogenesis of irritable bowel syndrome (IBS). This study used a molecular fingerprinting technique to compare the composition and biodiversity of the microbiota within fecal and mucosal niches between pati...

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Veröffentlicht in:American journal of physiology: Gastrointestinal and liver physiology Jg. 301; H. 5; S. G799
Hauptverfasser: Carroll, Ian M, Ringel-Kulka, Tamar, Keku, Temitope O, Chang, Young-Hyo, Packey, Christopher D, Sartor, R Balfour, Ringel, Yehuda
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.11.2011
Schlagworte:
Adult
Colon - microbiology
Diarrhea - microbiology
DNA, Bacterial - genetics
Feces - microbiology
Female
Humans
Intestinal Mucosa - microbiology
Irritable Bowel Syndrome - microbiology
Male
Metagenome - genetics
Middle Aged
ISSN:1522-1547, 1522-1547
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Zusammenfassung:Alterations in the intestinal microbiota have been suggested as an etiological factor in the pathogenesis of irritable bowel syndrome (IBS). This study used a molecular fingerprinting technique to compare the composition and biodiversity of the microbiota within fecal and mucosal niches between patients with diarrhea-predominant IBS (D-IBS) and healthy controls. Terminal-restriction fragment (T-RF) length polymorphism (T-RFLP) fingerprinting of the bacterial 16S rRNA gene was used to perform microbial community composition analyses on fecal and mucosal samples from patients with D-IBS (n = 16) and healthy controls (n = 21). Molecular fingerprinting of the microbiota from fecal and colonic mucosal samples revealed differences in the contribution of T-RFs to the microbiota between D-IBS patients and healthy controls. Further analysis revealed a significantly lower (1.2-fold) biodiversity of microbes within fecal samples from D-IBS patients than healthy controls (P = 0.008). No difference in biodiversity in mucosal samples was detected between D-IBS patients and healthy controls. Multivariate analysis of T-RFLP profiles demonstrated distinct microbial communities between luminal and mucosal niches in all samples. Our findings of compositional differences in the luminal- and mucosal-associated microbiota between D-IBS patients and healthy controls and diminished microbial biodiversity in D-IBS fecal samples further support the hypothesis that alterations in the intestinal microbiota may have an etiological role in the pathogenesis of D-IBS and suggest that luminal and mucosal niches need to be investigated.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1522-1547
1522-1547
DOI:10.1152/ajpgi.00154.2011