Morphological, genotypic, and physiological characterization of Acanthamoeba isolates from keratitis patients and the domestic environment in Vitoria, Espírito Santo, Brazil

•Acanthamoeba samples from Vitória, Brazil genotyped as T4 and T11 tolerated 37°C.•One keratitis isolate cultivated in NNA with E. coli survived at 42°C.•All isolates in NNA with E. coli, except one from environment, tolerated 1.0M.•Isolates under axenic condition did not grow at 0.5M and 1.0M.•Osmo...

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