N‐1 semi‐continuous transient perfusion in shake flask for ultra‐high density seeding of CHO cell cultures in benchtop bioreactors

One strategy to enhance the production of biological therapeutics is using transient perfusion in the preculture (N-1 stage) to seed the production culture (N stage) at ultra-high cell densities (>10 x 10⁶ viable cells/mL). This very high seeding density improves cell culture performance by short...

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Bibliographic Details
Published in:	Biotechnology progress Vol. 41; no. 5; pp. e70029 - n/a
Main Authors:	Lemire, Lucas, Reyes, Sebastian‐Juan, Durocher, Yves, Voyer, Robert, Henry, Olivier, Pham, Phuong Lan
Format:	Journal Article
Language:	English
Published:	Hoboken, USA Wiley 01.09.2025 John Wiley & Sons, Inc Wiley Subscription Services, Inc
Subjects:	Agitation Animals Bioreactors Cell Count Cell culture Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Cell death Cell density Cell Survival Centrifugation CHO cell culture CHO Cells Cricetinae Cricetulus Culture Media cumate inducible system Density Exchanging Filtration Flasks Oxygen - metabolism Oxygen transfer Perfusion Perfusion - methods preculture perfusion pseudo-perfusion semi-continuous perfusion Sulfite ultra-high seeding density (UHSD) pseudo‐perfusion preculture perfusion ultra‐high seeding density (UHSD) semi‐continuous perfusion oxygen transfer CHO cell culture cumate inducible system
ISSN:	8756-7938, 1520-6033, 1520-6033
Online Access:	Get full text
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