mRNA-Based Genetic Reprogramming

The discovery that ordinary skin cells can be turned into pluripotent stem cells by the forced expression of defined factors has raised hopes that personalized regenerative treatments based on immunologically compatible material derived from a patient's own cells might be realized in the not-to...

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Bibliographic Details
Published in:Molecular therapy Vol. 27; no. 4; p. 729
Main Authors: Warren, Luigi, Lin, Cory
Format: Journal Article
Language:English
Published: United States 10.04.2019
Subjects:
Cell Differentiation - genetics
Cellular Reprogramming - genetics
Fibroblasts - metabolism
Genetic Vectors
Humans
Induced Pluripotent Stem Cells - metabolism
Induced Pluripotent Stem Cells - transplantation
Nucleosides - genetics
RNA, Messenger - genetics
RNA, Small Interfering - genetics
Transfection
iPSCs
messenger RNA reprogramming
modified RNA
mRNA reprogramming
synthetic mRNA
cellular reprogramming
induced pluripotent stem cells
ISSN:1525-0024, 1525-0024
Online Access:Get more information
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Summary:The discovery that ordinary skin cells can be turned into pluripotent stem cells by the forced expression of defined factors has raised hopes that personalized regenerative treatments based on immunologically compatible material derived from a patient's own cells might be realized in the not-too-distant future. A major barrier to the clinical use of induced pluripotent stem cells (iPSCs) was initially presented by the need to employ integrating viral vectors to express the factors that induce an embryonic gene expression profile, which entails potentially oncogenic alteration of the normal genome. Several "non-integrating" reprogramming systems have been developed over the last decade to address this problem. Among these techniques, mRNA reprogramming is the most unambiguously "footprint-free," most productive, and perhaps the best suited to clinical production of stem cells. Herein, we discuss the origins of the mRNA-based reprogramming system, its benefits and drawbacks, recent technical improvements that simplify its application, and the status of current efforts to industrialize this approach to mass-produce human stem cells for the clinic.
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ISSN:1525-0024
1525-0024
DOI:10.1016/j.ymthe.2018.12.009