AF-2 activity and recruitment of steroid receptor coactivator 1 to the estrogen receptor depend on a lysine residue conserved in nuclear receptors

Hormone-dependent transcriptional activation by nuclear receptors depends on the presence of a conserved C-terminal amphipathic alpha-helix (helix 12) in the ligand-binding domain. Here we show that a lysine residue, which is conserved in most nuclear receptors in the predicted helix 3, is also requ...

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Vydané v:Molecular and cellular biology Ročník 17; číslo 4; s. 1832
Hlavní autori: Henttu, P M, Kalkhoven, E, Parker, M G
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States 01.04.1997
Predmet:
Adaptor Proteins, Signal Transducing
Amino Acid Sequence
Animals
Base Sequence
Cell Line
Conserved Sequence
Histone Acetyltransferases
Humans
Lysine - genetics
Mice
Molecular Sequence Data
Mutagenesis, Site-Directed
Nuclear Proteins - metabolism
Nuclear Receptor Coactivator 1
Nuclear Receptor Coactivator 2
Nuclear Receptor Interacting Protein 1
Oligodeoxyribonucleotides - genetics
Receptors, Cytoplasmic and Nuclear - chemistry
Receptors, Cytoplasmic and Nuclear - genetics
Receptors, Cytoplasmic and Nuclear - metabolism
Receptors, Estrogen - metabolism
Sequence Homology, Amino Acid
Transcription Factors - metabolism
Transcriptional Activation
ISSN:0270-7306
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Popis
Shrnutí:Hormone-dependent transcriptional activation by nuclear receptors depends on the presence of a conserved C-terminal amphipathic alpha-helix (helix 12) in the ligand-binding domain. Here we show that a lysine residue, which is conserved in most nuclear receptors in the predicted helix 3, is also required for estrogen-dependent transactivation. The replacement of lysine 366 with alanine appreciably reduced activation function 2 (AF-2) activity without affecting steroid- or DNA-binding activity in the mouse estrogen receptor. The mutation dramatically reduced the ability of the receptor to bind steroid receptor coactivator 1 (SRC-1) but had no effect on receptor-interacting protein 140 (RIP-140) binding, indicating that while their sites of interaction overlap, they are not entirely consistent and in keeping with the proposal that the recruitment of coactivators, such as SRC-1, is required for AF-2 activity. Although the function of RIP-140 remains to be established, RIP-140 appears to be capable of recruiting the basal transcription machinery, since overexpression of the protein markedly increased the transcriptional activity of the mutant receptor. Since the lysine residue is conserved, we propose that it is required, together with residues in helix 12, to form the surface by which members of the nuclear receptor family interact with coactivators.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0270-7306
DOI:10.1128/MCB.17.4.1832