Structure, Immunoreactivity, and In Silico Epitope Determination of SmSPI S. mansoni Serpin for Immunodiagnostic Application

The human parasitic disease Schistosomiasis is caused by the Schistosoma trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been observed as an emerging disease in Europe, due to increased imm...

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Vydáno v:Vaccines (Basel) Ročník 9; číslo 4; s. 322
Hlavní autoři: De Benedetti, Stefano, Di Pisa, Flavio, Fassi, Enrico Mario Alessandro, Cretich, Marina, Musicò, Angelo, Frigerio, Roberto, Mussida, Alessandro, Bombaci, Mauro, Grifantini, Renata, Colombo, Giorgio, Bolognesi, Martino, Grande, Romualdo, Zanchetta, Nadia, Gismondo, Maria Rita, Mileto, Davide, Mancon, Alessandro, Gourlay, Louise Jane
Médium: Journal Article
Jazyk:angličtina
Vydáno: Switzerland MDPI AG 01.04.2021
MDPI
Témata:
Antibodies
Antigens
Antisera
Biomarkers
Chromatography
circulating antigen
Crystal structure
Crystallization
Drug resistance
Epitope mapping
Fresh water
Immigration
Immunoreactivity
in silico epitope predictions
Infections
Neglected Tropical Disease
Parasites
Parasitic diseases
Pathogens
Protease inhibitors
Proteinase inhibitors
Proteins
Schistosoma
Schistosoma mansoni
Schistosomiasis
Serine
Serine protease inhibitor
Serine proteinase
Tropical diseases
Tropical environment
Tropical environments
United States > US
in silico epitope predictions
Neglected Tropical Disease
crystal structure
circulating antigen
immunodiagnostics
schistosomiasis
Serine protease inhibitor
ISSN:2076-393X, 2076-393X
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Shrnutí:The human parasitic disease Schistosomiasis is caused by the Schistosoma trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been observed as an emerging disease in Europe, due to increased immigration. In addition to improved therapeutic strategies, it is imperative to develop novel, rapid, and sensitive diagnostic tests that can detect the Schistosoma parasite, allowing timely treatment. Present diagnosis is difficult and involves microscopy-based detection of Schistosoma eggs in the feces. In this context, we present the 3.22 Å resolution crystal structure of the circulating antigen Serine protease inhibitor from S. mansoni (SmSPI), and we describe it as a potential serodiagnostic marker. Moreover, we identify three potential immunoreactive epitopes using in silico-based epitope mapping methods. Here, we confirm effective immune sera reactivity of the recombinant antigen, suggesting the further investigation of the protein and/or its predicted epitopes as serodiagnostic Schistosomiasis biomarkers.
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Current affiliation: Department of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy.
Current affiliation: Biocrystallography Unit, Division of Immunology, Transplantation, and Infectious Diseases, IRCCS Scientific Institute San Raffaele, 20132 Milan, Italy.
ISSN:2076-393X
2076-393X
DOI:10.3390/vaccines9040322