Structure, Immunoreactivity, and In Silico Epitope Determination of SmSPI S. mansoni Serpin for Immunodiagnostic Application

The human parasitic disease Schistosomiasis is caused by the Schistosoma trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been observed as an emerging disease in Europe, due to increased imm...

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Vydané v:Vaccines (Basel) Ročník 9; číslo 4; s. 322
Hlavní autori: De Benedetti, Stefano, Di Pisa, Flavio, Fassi, Enrico Mario Alessandro, Cretich, Marina, Musicò, Angelo, Frigerio, Roberto, Mussida, Alessandro, Bombaci, Mauro, Grifantini, Renata, Colombo, Giorgio, Bolognesi, Martino, Grande, Romualdo, Zanchetta, Nadia, Gismondo, Maria Rita, Mileto, Davide, Mancon, Alessandro, Gourlay, Louise Jane
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Switzerland MDPI AG 01.04.2021
MDPI
Predmet:
Antibodies
Antigens
Antisera
Biomarkers
Chromatography
circulating antigen
Crystal structure
Crystallization
Drug resistance
Epitope mapping
Fresh water
Immigration
Immunoreactivity
in silico epitope predictions
Infections
Neglected Tropical Disease
Parasites
Parasitic diseases
Pathogens
Protease inhibitors
Proteinase inhibitors
Proteins
Schistosoma
Schistosoma mansoni
Schistosomiasis
Serine
Serine protease inhibitor
Serine proteinase
Tropical diseases
Tropical environment
Tropical environments
United States > US
in silico epitope predictions
Neglected Tropical Disease
crystal structure
circulating antigen
immunodiagnostics
schistosomiasis
Serine protease inhibitor
ISSN:2076-393X, 2076-393X
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Abstract The human parasitic disease Schistosomiasis is caused by the Schistosoma trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been observed as an emerging disease in Europe, due to increased immigration. In addition to improved therapeutic strategies, it is imperative to develop novel, rapid, and sensitive diagnostic tests that can detect the Schistosoma parasite, allowing timely treatment. Present diagnosis is difficult and involves microscopy-based detection of Schistosoma eggs in the feces. In this context, we present the 3.22 Å resolution crystal structure of the circulating antigen Serine protease inhibitor from S. mansoni (SmSPI), and we describe it as a potential serodiagnostic marker. Moreover, we identify three potential immunoreactive epitopes using in silico-based epitope mapping methods. Here, we confirm effective immune sera reactivity of the recombinant antigen, suggesting the further investigation of the protein and/or its predicted epitopes as serodiagnostic Schistosomiasis biomarkers.
AbstractList The human parasitic disease Schistosomiasis is caused by the Schistosoma trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been observed as an emerging disease in Europe, due to increased immigration. In addition to improved therapeutic strategies, it is imperative to develop novel, rapid, and sensitive diagnostic tests that can detect the Schistosoma parasite, allowing timely treatment. Present diagnosis is difficult and involves microscopy-based detection of Schistosoma eggs in the feces. In this context, we present the 3.22 Å resolution crystal structure of the circulating antigen Serine protease inhibitor from S. mansoni (SmSPI), and we describe it as a potential serodiagnostic marker. Moreover, we identify three potential immunoreactive epitopes using in silico-based epitope mapping methods. Here, we confirm effective immune sera reactivity of the recombinant antigen, suggesting the further investigation of the protein and/or its predicted epitopes as serodiagnostic Schistosomiasis biomarkers.
The human parasitic disease Schistosomiasis is caused by the trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been observed as an emerging disease in Europe, due to increased immigration. In addition to improved therapeutic strategies, it is imperative to develop novel, rapid, and sensitive diagnostic tests that can detect the parasite, allowing timely treatment. Present diagnosis is difficult and involves microscopy-based detection of Schistosoma eggs in the feces. In this context, we present the 3.22 Å resolution crystal structure of the circulating antigen Serine protease inhibitor from (SmSPI), and we describe it as a potential serodiagnostic marker. Moreover, we identify three potential immunoreactive epitopes using in silico-based epitope mapping methods. Here, we confirm effective immune sera reactivity of the recombinant antigen, suggesting the further investigation of the protein and/or its predicted epitopes as serodiagnostic Schistosomiasis biomarkers.
The human parasitic disease Schistosomiasis is caused by the Schistosoma trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been observed as an emerging disease in Europe, due to increased immigration. In addition to improved therapeutic strategies, it is imperative to develop novel, rapid, and sensitive diagnostic tests that can detect the Schistosoma parasite, allowing timely treatment. Present diagnosis is difficult and involves microscopy-based detection of Schistosoma eggs in the feces. In this context, we present the 3.22 Å resolution crystal structure of the circulating antigen Serine protease inhibitor from S. mansoni (SmSPI), and we describe it as a potential serodiagnostic marker. Moreover, we identify three potential immunoreactive epitopes using in silico-based epitope mapping methods. Here, we confirm effective immune sera reactivity of the recombinant antigen, suggesting the further investigation of the protein and/or its predicted epitopes as serodiagnostic Schistosomiasis biomarkers.The human parasitic disease Schistosomiasis is caused by the Schistosoma trematode flatworm that infects freshwaters in tropical regions of the world, particularly in Sub-Saharan Africa, South America, and the Far-East. It has also been observed as an emerging disease in Europe, due to increased immigration. In addition to improved therapeutic strategies, it is imperative to develop novel, rapid, and sensitive diagnostic tests that can detect the Schistosoma parasite, allowing timely treatment. Present diagnosis is difficult and involves microscopy-based detection of Schistosoma eggs in the feces. In this context, we present the 3.22 Å resolution crystal structure of the circulating antigen Serine protease inhibitor from S. mansoni (SmSPI), and we describe it as a potential serodiagnostic marker. Moreover, we identify three potential immunoreactive epitopes using in silico-based epitope mapping methods. Here, we confirm effective immune sera reactivity of the recombinant antigen, suggesting the further investigation of the protein and/or its predicted epitopes as serodiagnostic Schistosomiasis biomarkers.
Author Musicò, Angelo
Grifantini, Renata
De Benedetti, Stefano
Frigerio, Roberto
Zanchetta, Nadia
Gourlay, Louise Jane
Bombaci, Mauro
Bolognesi, Martino
Gismondo, Maria Rita
Cretich, Marina
Mileto, Davide
Colombo, Giorgio
Grande, Romualdo
Mussida, Alessandro
Fassi, Enrico Mario Alessandro
Di Pisa, Flavio
Mancon, Alessandro
AuthorAffiliation 6 Centro di Ricerca Pediatrica Romeo ed Enrica Invernizzi, Università degli Studi di Milano, 20133 Milano, Italy
3 Dipartimento di Scienze Farmaceutiche, Università degli Studi di Milano, Via L. Mangiagalli 25, 20133 Milano, Italy
4 Istituto Nazionale Genetica Molecolare, Padiglione Romeo ed Enrica Invernizzi, IRCCS Ospedale Maggiore Policlinico, 20122 Milan, Italy; bombaci@ingm.org (M.B.); grifantini@ingm.org (R.G.)
5 Dipartimento di Chimica, Università di Pavia, V.le Taramelli 12, 27100 Pavia, Italy; g.colombo@unipv.it
1 Department of Biosciences, Università degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy; stefano.debenedetti@unimi.it (S.D.B.); DiPisa.Flavio@hsr.it (F.D.P.); martino.bolognesi@unimi.it (M.B.)
8 Clinical Microbiology, Virology and Bioemergency Unit, Department of Biomedical and Clinical Sciences, Luigi Sacco Hospital, University of Milan, 20157 Milan, Italy
2 Consiglio Nazionale delle Ricerche, Istituto di Scienze e Tecnologie Chimiche “Giulio Natta” (SCITEC), Via
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– name: 7 UOC Microbiologia Clinica, Virologia e Diagnostica delle Bioemergenze ASST FBF Sacco, 20157 Milano, Italy; grande.romualdo@asst-fbf-sacco.it (R.G.); nadia.zanchetta@asst-fbf-sacco.it (N.Z.); mariarita.gismondo@unimi.it (M.R.G.); davide.mileto@unimi.it (D.M.); alessandro.mancon@unimi.it (A.M.)
– name: 6 Centro di Ricerca Pediatrica Romeo ed Enrica Invernizzi, Università degli Studi di Milano, 20133 Milano, Italy
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/33915716$$D View this record in MEDLINE/PubMed
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Issue 4
Keywords in silico epitope predictions
Neglected Tropical Disease
crystal structure
circulating antigen
immunodiagnostics
schistosomiasis
Serine protease inhibitor
Language English
License Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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Current affiliation: Department of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy.
Current affiliation: Biocrystallography Unit, Division of Immunology, Transplantation, and Infectious Diseases, IRCCS Scientific Institute San Raffaele, 20132 Milan, Italy.
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Snippet The human parasitic disease Schistosomiasis is caused by the Schistosoma trematode flatworm that infects freshwaters in tropical regions of the world,...
The human parasitic disease Schistosomiasis is caused by the trematode flatworm that infects freshwaters in tropical regions of the world, particularly in...
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StartPage 322
SubjectTerms Antibodies
Antigens
Antisera
Biomarkers
Chromatography
circulating antigen
Crystal structure
Crystallization
Drug resistance
Epitope mapping
Fresh water
Immigration
Immunoreactivity
in silico epitope predictions
Infections
Neglected Tropical Disease
Parasites
Parasitic diseases
Pathogens
Protease inhibitors
Proteinase inhibitors
Proteins
Schistosoma
Schistosoma mansoni
Schistosomiasis
Serine
Serine protease inhibitor
Serine proteinase
Tropical diseases
Tropical environment
Tropical environments
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Title Structure, Immunoreactivity, and In Silico Epitope Determination of SmSPI S. mansoni Serpin for Immunodiagnostic Application
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