Sister chromatid cohesion establishment during DNA replication termination

Newly copied sister chromatids are tethered together by the cohesin complex, but how sister chromatid cohesion coordinates with DNA replication is poorly understood. Prevailing models suggest that cohesin complexes, bound to DNA before replication, remain behind the advancing replication fork to kee...

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Published in:Science (American Association for the Advancement of Science) Vol. 384; no. 6691; p. 119
Main Authors: Cameron, George, Gruszka, Dominika T, Gruar, Rhian, Xie, Sherry, Kaya, Çağla, Nasmyth, Kim A, Baxter, Jonathan, Srinivasan, Madhusudhan, Yardimci, Hasan
Format: Journal Article
Language:English
Published: United States 05.04.2024
Subjects:
Chromatids - metabolism
Chromosomal Proteins, Non-Histone - metabolism
Cohesins - metabolism
DNA Replication
DNA-Binding Proteins - metabolism
Minichromosome Maintenance Proteins - metabolism
Nuclear Proteins - metabolism
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Sister Chromatid Exchange
ISSN:1095-9203, 1095-9203
Online Access:Get more information
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Summary:Newly copied sister chromatids are tethered together by the cohesin complex, but how sister chromatid cohesion coordinates with DNA replication is poorly understood. Prevailing models suggest that cohesin complexes, bound to DNA before replication, remain behind the advancing replication fork to keep sister chromatids together. By visualizing single replication forks colliding with preloaded cohesin complexes, we find that the replisome instead pushes cohesin to where a converging replisome is met. Whereas the converging replisomes are removed during DNA replication termination, cohesin remains on nascent DNA and provides cohesion. Additionally, we show that CMG (CDC45-MCM2-7-GINS) helicase disassembly during replication termination is vital for proper cohesion in budding yeast. Together, our results support a model wherein sister chromatid cohesion is established during DNA replication termination.
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ISSN:1095-9203
1095-9203
DOI:10.1126/science.adf0224