Cell competition during reprogramming gives rise to dominant clones

The ability to generate induced pluripotent stem cells from differentiated cell types has enabled researchers to engineer cell states. Although studies have identified molecular networks that reprogram cells to pluripotency, the cellular dynamics of these processes remain poorly understood. Here, by...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Science (American Association for the Advancement of Science) Ročník 364; číslo 6438
Hlavní autoři: Shakiba, Nika, Fahmy, Ahmed, Jayakumaran, Gowtham, McGibbon, Sophie, David, Laurent, Trcka, Daniel, Elbaz, Judith, Puri, Mira C, Nagy, Andras, van der Kooy, Derek, Goyal, Sidhartha, Wrana, Jeffrey L, Zandstra, Peter W
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States 26.04.2019
Témata:
Animals
Cellular Reprogramming - genetics
Cellular Reprogramming - physiology
Cellular Reprogramming Techniques
Clonal Evolution
Clone Cells - cytology
DNA - genetics
Fibroblasts - cytology
Induced Pluripotent Stem Cells - cytology
Mice
Models, Theoretical
ISSN:1095-9203, 1095-9203
On-line přístup:Zjistit podrobnosti o přístupu
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Abstract The ability to generate induced pluripotent stem cells from differentiated cell types has enabled researchers to engineer cell states. Although studies have identified molecular networks that reprogram cells to pluripotency, the cellular dynamics of these processes remain poorly understood. Here, by combining cellular barcoding, mathematical modeling, and lineage tracing approaches, we demonstrate that reprogramming dynamics in heterogeneous populations are driven by dominant "elite" clones. Clones arise a priori from a population of poised mouse embryonic fibroblasts derived from Wnt1-expressing cells that may represent a neural crest-derived population. This work highlights the importance of cellular dynamics in fate programming outcomes and uncovers cell competition as a mechanism by which cells with eliteness emerge to occupy and dominate the reprogramming niche.
AbstractList The ability to generate induced pluripotent stem cells from differentiated cell types has enabled researchers to engineer cell states. Although studies have identified molecular networks that reprogram cells to pluripotency, the cellular dynamics of these processes remain poorly understood. Here, by combining cellular barcoding, mathematical modeling, and lineage tracing approaches, we demonstrate that reprogramming dynamics in heterogeneous populations are driven by dominant "elite" clones. Clones arise a priori from a population of poised mouse embryonic fibroblasts derived from Wnt1-expressing cells that may represent a neural crest-derived population. This work highlights the importance of cellular dynamics in fate programming outcomes and uncovers cell competition as a mechanism by which cells with eliteness emerge to occupy and dominate the reprogramming niche.The ability to generate induced pluripotent stem cells from differentiated cell types has enabled researchers to engineer cell states. Although studies have identified molecular networks that reprogram cells to pluripotency, the cellular dynamics of these processes remain poorly understood. Here, by combining cellular barcoding, mathematical modeling, and lineage tracing approaches, we demonstrate that reprogramming dynamics in heterogeneous populations are driven by dominant "elite" clones. Clones arise a priori from a population of poised mouse embryonic fibroblasts derived from Wnt1-expressing cells that may represent a neural crest-derived population. This work highlights the importance of cellular dynamics in fate programming outcomes and uncovers cell competition as a mechanism by which cells with eliteness emerge to occupy and dominate the reprogramming niche.
The ability to generate induced pluripotent stem cells from differentiated cell types has enabled researchers to engineer cell states. Although studies have identified molecular networks that reprogram cells to pluripotency, the cellular dynamics of these processes remain poorly understood. Here, by combining cellular barcoding, mathematical modeling, and lineage tracing approaches, we demonstrate that reprogramming dynamics in heterogeneous populations are driven by dominant "elite" clones. Clones arise a priori from a population of poised mouse embryonic fibroblasts derived from Wnt1-expressing cells that may represent a neural crest-derived population. This work highlights the importance of cellular dynamics in fate programming outcomes and uncovers cell competition as a mechanism by which cells with eliteness emerge to occupy and dominate the reprogramming niche.
Author Wrana, Jeffrey L
Jayakumaran, Gowtham
Fahmy, Ahmed
David, Laurent
Elbaz, Judith
Goyal, Sidhartha
McGibbon, Sophie
Shakiba, Nika
Puri, Mira C
van der Kooy, Derek
Trcka, Daniel
Nagy, Andras
Zandstra, Peter W
Author_xml – sequence: 1
  givenname: Nika
  orcidid: 0000-0003-2985-3130
  surname: Shakiba
  fullname: Shakiba, Nika
  organization: Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario M5S 3E1, Canada
– sequence: 2
  givenname: Ahmed
  surname: Fahmy
  fullname: Fahmy, Ahmed
  organization: Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada
– sequence: 3
  givenname: Gowtham
  surname: Jayakumaran
  fullname: Jayakumaran, Gowtham
  organization: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
– sequence: 4
  givenname: Sophie
  orcidid: 0000-0002-4071-912X
  surname: McGibbon
  fullname: McGibbon, Sophie
  organization: Department of Physics, University of Toronto, Toronto, Ontario M5S 1A7, Canada
– sequence: 5
  givenname: Laurent
  surname: David
  fullname: David, Laurent
  organization: ITUN, CHU Nantes, Nantes, France
– sequence: 6
  givenname: Daniel
  orcidid: 0000-0002-2196-7160
  surname: Trcka
  fullname: Trcka, Daniel
  organization: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
– sequence: 7
  givenname: Judith
  surname: Elbaz
  fullname: Elbaz, Judith
  organization: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
– sequence: 8
  givenname: Mira C
  orcidid: 0000-0003-0799-6122
  surname: Puri
  fullname: Puri, Mira C
  organization: Department of Medical Biophysics, University of Toronto, Toronto, Ontario M5T 3H7, Canada
– sequence: 9
  givenname: Andras
  orcidid: 0000-0003-4311-0413
  surname: Nagy
  fullname: Nagy, Andras
  organization: Australian Regenerative Medicine Institute, Monash University, Melbourne, Victoria, Australia
– sequence: 10
  givenname: Derek
  surname: van der Kooy
  fullname: van der Kooy, Derek
  organization: The Donnelly Centre for Cellular and Biomolecular Research (CCBR), University of Toronto, Toronto, Ontario M5S 3E1, Canada
– sequence: 11
  givenname: Sidhartha
  surname: Goyal
  fullname: Goyal, Sidhartha
  organization: Department of Physics, University of Toronto, Toronto, Ontario M5S 1A7, Canada
– sequence: 12
  givenname: Jeffrey L
  orcidid: 0000-0003-0932-0644
  surname: Wrana
  fullname: Wrana, Jeffrey L
  organization: Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
– sequence: 13
  givenname: Peter W
  orcidid: 0000-0002-9568-733X
  surname: Zandstra
  fullname: Zandstra, Peter W
  email: peter.zandstra@ubc.ca
  organization: Michael Smith Laboratories, The University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada
BackLink https://www.ncbi.nlm.nih.gov/pubmed/30898844$$D View this record in MEDLINE/PubMed
BookMark eNpNj01LxDAQhoOsuB969iY5eumapE3THKW4Kix40XPJJrMl0iQ1aQX_vRFX8DTzDg8vz6zRwgcPCF1TsqWU1XdJW_Aatkp5Ihk_QytKJC8kI-Xi375E65TeCclZlhdoWZJGNk1VrVDbwjBgHdwIk51s8NjM0foeRxhj6KNy7if19hMSjjYBngI2IR-Vn7Aesk66ROdHNSS4Os0Nets9vLZPxf7l8bm93xe6asRUMC4qZSrDGZUgaX00GnglDkzrstRCS2aMqKUwACJTSnEQjeG1UEICryXboNvf3mz2MUOaOmeTzv7KQ5hTl3vrXN4QkdGbEzofHJhujNap-NX9Pc6-AWTYXk4
CitedBy_id crossref_primary_10_1016_j_crmeth_2021_100087
crossref_primary_10_1089_cell_2024_0035
crossref_primary_10_1016_j_gde_2021_09_006
crossref_primary_10_1111_prd_12602
crossref_primary_10_1016_j_ydbio_2021_03_025
crossref_primary_10_1016_j_scr_2021_102195
crossref_primary_10_4103_1673_5374_290891
crossref_primary_10_1016_j_addr_2022_114421
crossref_primary_10_1038_s41392_024_01809_0
crossref_primary_10_1016_j_ymthe_2021_04_007
crossref_primary_10_1016_j_tree_2021_08_011
crossref_primary_10_1016_j_cels_2021_05_011
crossref_primary_10_1038_s41598_022_20089_8
crossref_primary_10_3389_fcell_2021_681087
crossref_primary_10_3390_cells13121010
crossref_primary_10_1038_s41467_022_35180_x
crossref_primary_10_1016_j_cels_2025_101300
crossref_primary_10_1016_j_celrep_2025_116226
crossref_primary_10_1177_10732748221078160
crossref_primary_10_1073_pnas_2207824119
crossref_primary_10_1016_j_bbadis_2025_167769
crossref_primary_10_1038_s41576_019_0123_5
crossref_primary_10_1016_j_scr_2020_102062
crossref_primary_10_1016_j_cobme_2021_100317
crossref_primary_10_1089_genbio_2022_0001
crossref_primary_10_1016_j_gde_2020_05_016
crossref_primary_10_1038_s41586_021_03273_0
crossref_primary_10_1016_j_cels_2024_01_001
crossref_primary_10_1186_s12915_020_00911_3
crossref_primary_10_15252_embj_2020107271
crossref_primary_10_1038_s41587_019_0227_7
crossref_primary_10_3389_fcell_2021_662078
crossref_primary_10_1016_j_nlm_2020_107318
crossref_primary_10_1016_j_coisb_2020_09_002
crossref_primary_10_1016_j_gde_2021_06_015
crossref_primary_10_1038_s41467_023_37270_w
crossref_primary_10_3389_fcell_2025_1672074
crossref_primary_10_1016_j_bpj_2019_10_014
crossref_primary_10_3389_fendo_2023_1235614
crossref_primary_10_1016_j_gde_2021_05_008
crossref_primary_10_1038_s41587_020_0614_0
crossref_primary_10_1016_j_cell_2020_11_018
crossref_primary_10_1088_1758_5090_ac23ae
crossref_primary_10_1242_dev_202730
crossref_primary_10_1016_j_animal_2023_100803
crossref_primary_10_1146_annurev_cancerbio_030419_033413
crossref_primary_10_1016_j_tcb_2020_10_002
crossref_primary_10_1016_j_cels_2025_101205
crossref_primary_10_1016_j_cels_2025_101324
crossref_primary_10_3389_fcell_2022_891569
crossref_primary_10_15252_embr_202255678
crossref_primary_10_1126_science_aax1681
crossref_primary_10_3390_cells10112888
crossref_primary_10_1016_j_pneurobio_2020_101966
crossref_primary_10_1073_pnas_2204394119
crossref_primary_10_1002_stem_3295
crossref_primary_10_1002_advs_202003516
crossref_primary_10_1146_annurev_chembioeng_100722_121610
crossref_primary_10_1155_2019_8036035
crossref_primary_10_15252_msb_20199195
crossref_primary_10_1038_s41567_021_01383_0
crossref_primary_10_3389_fcell_2022_1054476
crossref_primary_10_1016_j_stem_2024_01_007
crossref_primary_10_1016_j_tig_2020_04_003
crossref_primary_10_1089_genbio_2021_0014
ContentType Journal Article
Copyright Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
Copyright_xml – notice: Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
DBID CGR
CUY
CVF
ECM
EIF
NPM
7X8
DOI 10.1126/science.aan0925
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
MEDLINE - Academic
DatabaseTitleList MEDLINE - Academic
MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: 7X8
  name: MEDLINE - Academic
  url: https://search.proquest.com/medline
  sourceTypes: Aggregation Database
DeliveryMethod no_fulltext_linktorsrc
Discipline Sciences (General)
Biology
EISSN 1095-9203
ExternalDocumentID 30898844
Genre Research Support, Non-U.S. Gov't
Journal Article
GroupedDBID ---
--Z
-DZ
-ET
-~X
.-4
..I
.55
.DC
08G
0R~
0WA
123
18M
2FS
2KS
2WC
34G
36B
39C
3R3
53G
5RE
66.
6OB
6TJ
7X2
7~K
85S
8F7
AABCJ
AACGO
AAIKC
AAMNW
AANCE
AAWTO
ABCQX
ABDBF
ABDEX
ABDQB
ABEFU
ABIVO
ABJNI
ABOCM
ABPLY
ABPPZ
ABQIJ
ABTLG
ABWJO
ABZEH
ACBEA
ACBEC
ACGFO
ACGFS
ACGOD
ACIWK
ACMJI
ACNCT
ACPRK
ACQOY
ACUHS
ADDRP
ADUKH
AEGBM
AENEX
AETEA
AEUPB
AFFDN
AFFNX
AFHKK
AFQFN
AFRAH
AGFXO
AGNAY
AGSOS
AHMBA
AIDAL
AIDUJ
AJGZS
ALIPV
ALMA_UNASSIGNED_HOLDINGS
ASPBG
AVWKF
BKF
BLC
C45
CGR
CS3
CUY
CVF
DB2
DU5
EBS
ECM
EIF
EJD
EMOBN
F5P
FA8
FEDTE
GX1
HZ~
I.T
IAO
IEA
IGS
IH2
IHR
INH
INR
IOF
IOV
IPO
IPY
ISE
JCF
JLS
JSG
JST
K-O
KCC
L7B
LSO
LU7
M0P
MQT
MVM
N9A
NEJ
NHB
NPM
O9-
OCB
OFXIZ
OGEVE
OK1
OMK
OVD
P-O
P2P
PQQKQ
PZZ
QS-
RHI
RXW
SC5
SJN
TAE
TEORI
TN5
TWZ
UBW
UCV
UHB
UIG
UKR
UMD
UNMZH
UQL
USG
VVN
WH7
WI4
X7M
XJF
XZL
Y6R
YCJ
YK4
YKV
YNT
YOJ
YR2
YR5
YRY
YSQ
YV5
YWH
YYP
YZZ
ZCA
ZE2
ZKG
~02
~G0
~KM
~ZZ
2XV
7X8
ABBHK
ADXHL
AEXZC
AFBNE
ATCPS
DCCCD
IPSME
JAAYA
JBMMH
JENOY
JHFFW
JKQEH
JLXEF
JPM
SA0
ID FETCH-LOGICAL-c487t-2574ad4d5219e916fdce547b2cc33c7c92dd7697dee74d5aa5e78d567a79e5692
IEDL.DBID 7X8
ISICitedReferencesCount 77
ISICitedReferencesURI http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000465643400039&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN 1095-9203
IngestDate Sun Nov 09 12:32:03 EST 2025
Thu Apr 03 07:05:33 EDT 2025
IsPeerReviewed true
IsScholarly true
Issue 6438
Language English
License Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c487t-2574ad4d5219e916fdce547b2cc33c7c92dd7697dee74d5aa5e78d567a79e5692
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ORCID 0000-0003-2985-3130
0000-0003-4311-0413
0000-0002-9568-733X
0000-0003-0799-6122
0000-0003-0932-0644
0000-0002-2196-7160
0000-0002-4071-912X
PMID 30898844
PQID 2196521807
PQPubID 23479
ParticipantIDs proquest_miscellaneous_2196521807
pubmed_primary_30898844
PublicationCentury 2000
PublicationDate 2019-04-26
PublicationDateYYYYMMDD 2019-04-26
PublicationDate_xml – month: 04
  year: 2019
  text: 2019-04-26
  day: 26
PublicationDecade 2010
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle Science (American Association for the Advancement of Science)
PublicationTitleAlternate Science
PublicationYear 2019
References 30940927 - Nat Rev Genet. 2019 Jun;20(6):321
31023911 - Science. 2019 Apr 26;364(6438):330-331
References_xml – reference: 31023911 - Science. 2019 Apr 26;364(6438):330-331
– reference: 30940927 - Nat Rev Genet. 2019 Jun;20(6):321
SSID ssj0009593
Score 2.549313
Snippet The ability to generate induced pluripotent stem cells from differentiated cell types has enabled researchers to engineer cell states. Although studies have...
SourceID proquest
pubmed
SourceType Aggregation Database
Index Database
SubjectTerms Animals
Cellular Reprogramming - genetics
Cellular Reprogramming - physiology
Cellular Reprogramming Techniques
Clonal Evolution
Clone Cells - cytology
DNA - genetics
Fibroblasts - cytology
Induced Pluripotent Stem Cells - cytology
Mice
Models, Theoretical
Title Cell competition during reprogramming gives rise to dominant clones
URI https://www.ncbi.nlm.nih.gov/pubmed/30898844
https://www.proquest.com/docview/2196521807
Volume 364
WOSCitedRecordID wos000465643400039&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText
inHoldings 1
isFullTextHit
isPrint
link http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LS8QwEB7UVfCirs_1RQQPeqj2kSbNSWRx8eKyB4W9lTRJRVjb1a6C_95JmkURBMFLoZCWMMzjSzL5PoBTWghE7QWzMqkswEg0ARZBFsiQSouQEx5qJzbBh8NsPBYjv-HW-LbKeU50iVrXyu6RX8aW-g7rUcivpi-BVY2yp6teQmMROglCGevVfJz9JN2NQqtHGIeJp_b5dmnmQsoqFHH6O750dWaw_t8ZbsCaR5jkunWJLiyYahNWWs3Jj03o-mhuyJmnnD7fgn7fTCZEOQzterhIe32RWM5L18D1bN8eLUctwbRgyKwmum7baIiaWML_bXgY3Nz3bwMvrxAoXKXMAgxWKjXVOGdhECWWWpmU8iJWKkkUVyLWmjPBtTEcR0mZGp7plHHJhUmZiHdgqcL_7wHBNUphWGmiRFoGQSmKUOgoKnF8yRQtenAyN1mO7mvPJGRl6rcm_zJaD3Zbu-fTlmcjT8JMZBml-3_4-gBWEcq4c56YHUKnxOA1R7Cs3mdPzeux8wt8Dkd3nyTRwr0
linkProvider ProQuest
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Cell+competition+during+reprogramming+gives+rise+to+dominant+clones&rft.jtitle=Science+%28American+Association+for+the+Advancement+of+Science%29&rft.au=Shakiba%2C+Nika&rft.au=Fahmy%2C+Ahmed&rft.au=Jayakumaran%2C+Gowtham&rft.au=McGibbon%2C+Sophie&rft.date=2019-04-26&rft.eissn=1095-9203&rft.volume=364&rft.issue=6438&rft_id=info:doi/10.1126%2Fscience.aan0925&rft_id=info%3Apmid%2F30898844&rft_id=info%3Apmid%2F30898844&rft.externalDocID=30898844
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1095-9203&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1095-9203&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1095-9203&client=summon