Emergence of Usutu virus in Hungary
In 2001, Usutu virus (USUV), a mosquito-borne flavivirus of the Japanese encephalitis virus serogroup related to West Nile virus and previously restricted to sub-Saharan Africa, emerged in wild and zoo birds in and around Vienna, Austria. In order to monitor the spread of the infection, a dead bird...
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| Veröffentlicht in: | Journal of clinical microbiology Jg. 45; H. 12; S. 3870 |
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01.12.2007
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| Abstract | In 2001, Usutu virus (USUV), a mosquito-borne flavivirus of the Japanese encephalitis virus serogroup related to West Nile virus and previously restricted to sub-Saharan Africa, emerged in wild and zoo birds in and around Vienna, Austria. In order to monitor the spread of the infection, a dead bird surveillance program was established in Austria and in neighboring Hungary. In Hungary, 332 dead birds belonging to 52 species were tested for USUV infection between 2003 and 2006. In the first 2 years, all birds investigated were negative. In August 2005, however, USUV was detected in organ samples of a blackbird (Turdus merula), which was found dead in Budapest, Hungary, by reverse transcription-PCR, immunohistochemistry, and in situ hybridization. In July and August 2006, a further six dead blackbirds tested positive for USUV, and the virus was isolated from organ samples of one bird. These birds were also found in urban areas of Budapest. The nearly complete genomic sequence of one Hungarian USUV strain was determined; it was found to share 99.9% identity with the strain that has been circulating in Austria since 2001. This result indicates that the USUV strain responsible for the blackbird die-off in Budapest most likely spread from Austria to Hungary instead of being independently introduced from Africa. |
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| AbstractList | In 2001, Usutu virus (USUV), a mosquito-borne flavivirus of the Japanese encephalitis virus serogroup related to West Nile virus and previously restricted to sub-Saharan Africa, emerged in wild and zoo birds in and around Vienna, Austria. In order to monitor the spread of the infection, a dead bird surveillance program was established in Austria and in neighboring Hungary. In Hungary, 332 dead birds belonging to 52 species were tested for USUV infection between 2003 and 2006. In the first 2 years, all birds investigated were negative. In August 2005, however, USUV was detected in organ samples of a blackbird (Turdus merula), which was found dead in Budapest, Hungary, by reverse transcription-PCR, immunohistochemistry, and in situ hybridization. In July and August 2006, a further six dead blackbirds tested positive for USUV, and the virus was isolated from organ samples of one bird. These birds were also found in urban areas of Budapest. The nearly complete genomic sequence of one Hungarian USUV strain was determined; it was found to share 99.9% identity with the strain that has been circulating in Austria since 2001. This result indicates that the USUV strain responsible for the blackbird die-off in Budapest most likely spread from Austria to Hungary instead of being independently introduced from Africa. In 2001, Usutu virus (USUV), a mosquito-borne flavivirus of the Japanese encephalitis virus serogroup related to West Nile virus and previously restricted to sub-Saharan Africa, emerged in wild and zoo birds in and around Vienna, Austria. In order to monitor the spread of the infection, a dead bird surveillance program was established in Austria and in neighboring Hungary. In Hungary, 332 dead birds belonging to 52 species were tested for USUV infection between 2003 and 2006. In the first 2 years, all birds investigated were negative. In August 2005, however, USUV was detected in organ samples of a blackbird (Turdus merula), which was found dead in Budapest, Hungary, by reverse transcription-PCR, immunohistochemistry, and in situ hybridization. In July and August 2006, a further six dead blackbirds tested positive for USUV, and the virus was isolated from organ samples of one bird. These birds were also found in urban areas of Budapest. The nearly complete genomic sequence of one Hungarian USUV strain was determined; it was found to share 99.9% identity with the strain that has been circulating in Austria since 2001. This result indicates that the USUV strain responsible for the blackbird die-off in Budapest most likely spread from Austria to Hungary instead of being independently introduced from Africa.In 2001, Usutu virus (USUV), a mosquito-borne flavivirus of the Japanese encephalitis virus serogroup related to West Nile virus and previously restricted to sub-Saharan Africa, emerged in wild and zoo birds in and around Vienna, Austria. In order to monitor the spread of the infection, a dead bird surveillance program was established in Austria and in neighboring Hungary. In Hungary, 332 dead birds belonging to 52 species were tested for USUV infection between 2003 and 2006. In the first 2 years, all birds investigated were negative. In August 2005, however, USUV was detected in organ samples of a blackbird (Turdus merula), which was found dead in Budapest, Hungary, by reverse transcription-PCR, immunohistochemistry, and in situ hybridization. In July and August 2006, a further six dead blackbirds tested positive for USUV, and the virus was isolated from organ samples of one bird. These birds were also found in urban areas of Budapest. The nearly complete genomic sequence of one Hungarian USUV strain was determined; it was found to share 99.9% identity with the strain that has been circulating in Austria since 2001. This result indicates that the USUV strain responsible for the blackbird die-off in Budapest most likely spread from Austria to Hungary instead of being independently introduced from Africa. |
| Author | Csörgo, Tibor Bukovsky, Christiane Lussy, Helga Chvala, Sonja Ferenczi, Emoke Nowotny, Norbert Bakonyi, Tamás Erdélyi, Károly Ursu, Krisztina Meister, Tanja Weissenböck, Herbert |
| Author_xml | – sequence: 1 givenname: Tamás surname: Bakonyi fullname: Bakonyi, Tamás email: Bakonyi.Tamas@aotk.szie.hu organization: Department of Microbiology and Infectious Diseases, Faculty of Veterinary Science, Szent István University, Hungária krt 23-25, H-1143, Budapest, Hungary. Bakonyi.Tamas@aotk.szie.hu – sequence: 2 givenname: Károly surname: Erdélyi fullname: Erdélyi, Károly – sequence: 3 givenname: Krisztina surname: Ursu fullname: Ursu, Krisztina – sequence: 4 givenname: Emoke surname: Ferenczi fullname: Ferenczi, Emoke – sequence: 5 givenname: Tibor surname: Csörgo fullname: Csörgo, Tibor – sequence: 6 givenname: Helga surname: Lussy fullname: Lussy, Helga – sequence: 7 givenname: Sonja surname: Chvala fullname: Chvala, Sonja – sequence: 8 givenname: Christiane surname: Bukovsky fullname: Bukovsky, Christiane – sequence: 9 givenname: Tanja surname: Meister fullname: Meister, Tanja – sequence: 10 givenname: Herbert surname: Weissenböck fullname: Weissenböck, Herbert – sequence: 11 givenname: Norbert surname: Nowotny fullname: Nowotny, Norbert |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/17913929$$D View this record in MEDLINE/PubMed |
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| References | 15276857 - J Comp Pathol. 2004 Aug-Oct;131(2-3):176-85 15464850 - Virology. 2004 Oct 25;328(2):301-10 13679615 - J Gen Virol. 2003 Oct;84(Pt 10):2807-17 14554255 - Microbes Infect. 2003 Oct;5(12):1132-6 17869454 - Vet Microbiol. 2008 Mar 18;127(3-4):237-48 16236570 - Avian Pathol. 2005 Oct;34(5):392-5 16952307 - Virol J. 2006;3:71 17346908 - Vet Microbiol. 2007 Jun 21;122(3-4):237-45 9396791 - Nucleic Acids Res. 1997 Dec 15;25(24):4876-82 16629984 - J Vet Med B Infect Dis Vet Public Health. 2006 May;53(4):171-5 15752450 - Emerg Infect Dis. 2005 Feb;11(2):298-301 15372281 - Acta Neuropathol. 2004 Nov;108(5):453-60 17690413 - Am J Trop Med Hyg. 2007 Aug;77(2):358-64 17627436 - Vector Borne Zoonotic Dis. 2007 Summer;7(2):181-8 12095429 - Emerg Infect Dis. 2002 Jul;8(7):652-6 |
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| SubjectTerms | Animals Bird Diseases - virology Brain - pathology Brain - virology Flavivirus - isolation & purification Flavivirus Infections - pathology Flavivirus Infections - virology Heart - virology Hungary Immunohistochemistry In Situ Hybridization Liver - pathology Liver - virology Molecular Epidemiology Molecular Sequence Data Myocardium - pathology Passeriformes - virology Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - genetics Sequence Analysis, DNA Sequence Homology |
| Title | Emergence of Usutu virus in Hungary |
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