Mobile Efficient Diagnostics of Infectious Diseases via On‐Chip RT‐qPCR: MEDIC‐PCR

The COVID‐19 outbreak has caused public and global health crises. However, the lack of on‐site fast, reliable, sensitive, and low‐cost reverse transcription polymerase chain reaction (RT‐PCR) testing limits early detection, timely isolation, and epidemic prevention and control. Here, the authors rep...

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Vydáno v:Advanced science Ročník 10; číslo 28; s. e2302072 - n/a
Hlavní autoři: Shrestha, Kiran, Kim, Seongryeong, Han, Jiyeon, Florez, Gabriela Morales, Truong, Han, Hoang, Trung, Parajuli, Sajjan, AM, Tiara, Kim, Beomsoo, Jung, Younsu, Abafogi, Abdurhaman Teyib, Lee, Yugyeong, Song, Seung Hyun, Lee, Jinkee, Park, Sungsu, Kang, Minhee, Huh, Hee Jae, Cho, Gyoujin, Lee, Luke P.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Germany John Wiley & Sons, Inc 01.10.2023
John Wiley and Sons Inc
Wiley
Témata:
Accuracy
Carbon
Communicable Diseases - diagnosis
COVID-19
COVID-19 - diagnosis
COVID-19 Testing
diagnostics
Efficiency
Graphene
Humans
Infectious diseases
Light
low‐cost
Manufacturing
Polyethylene terephthalate
Polymerase Chain Reaction
rapid
Reverse Transcriptase Polymerase Chain Reaction
rt‐qpcr
Sensitivity and Specificity
Severe acute respiratory syndrome coronavirus 2
Thermal energy
Thin films
rapid
covid-19
diagnostics
low-cost
rt-qpcr
ISSN:2198-3844, 2198-3844
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Shrnutí:The COVID‐19 outbreak has caused public and global health crises. However, the lack of on‐site fast, reliable, sensitive, and low‐cost reverse transcription polymerase chain reaction (RT‐PCR) testing limits early detection, timely isolation, and epidemic prevention and control. Here, the authors report a rapid m obile e fficient d iagnostics of i nfectious diseases via on‐ c hip ‐RT‐quantitative PCR (RT‐qPCR): MEDIC‐PCR. First, the authors use a roll‐to‐roll printing process to accomplish low‐cost carbon‐black‐based disposable PCR chips that enable rapid LED‐induced photothermal PCR cycles. The MEDIC‐PCR can perform RT (3 min), and PCR (9 min) steps. Further, the cohort of 89 COVID‐19 and 103 non‐COVID‐19 patients testing is completed by the MEDIC‐PCR to show excellent diagnostic accuracy of 97%, sensitivity of 94%, and specificity of 98%. This MEDIC‐PCR can contribute to the preventive global health in the face of a future pandemic.
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ISSN:2198-3844
2198-3844
DOI:10.1002/advs.202302072