Detection of circulating epithelial cells in the blood of patients with breast cancer: comparison of three techniques

This study compares the sensitivities and specificities of three techniques for the detection of circulating epithelial cells in the blood of patients with breast cancer. The number of circulating epithelial cells present in the blood of 40 patients with metastatic breast cancer and 20 healthy volun...

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Vydáno v:	British journal of cancer Ročník 92; číslo 5; s. 906 - 912
Hlavní autoři:	Ring, A E, Zabaglo, L, Ormerod, M G, Smith, I E, Dowsett, M
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 14.03.2005 Nature Publishing Group
Témata:	Adult Aged Base Sequence Biological and medical sciences Biomedical and Life Sciences Biomedicine Breast Neoplasms - blood Breast Neoplasms - pathology Cancer Research DNA Primers Drug Resistance Epidemiology Epithelial Cells - pathology Female Humans Keratins - analysis Lymphatic Metastasis Medical sciences Middle Aged Molecular Diagnostics Molecular Medicine Neoplasm Metastasis Neoplasm Staging Oncology Reference Values Reverse Transcriptase Polymerase Chain Reaction Tumors breast cancer circulating tumour cells micrometastases Human Mammary gland diseases Blood cell Cancerology Epithelial cell Breast cancer Diagnosis Technique Malignant tumor Comparative study
ISSN:	0007-0920, 1532-1827
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Abstract	This study compares the sensitivities and specificities of three techniques for the detection of circulating epithelial cells in the blood of patients with breast cancer. The number of circulating epithelial cells present in the blood of 40 patients with metastatic breast cancer and 20 healthy volunteers was determined by: immunomagnetic separation (IMS) and laser scanning cytometry (LSC), cell filtration and LSC and a multimarker real-time RT–PCR assay. Numbers of cytokeratin-positive cells identified and expression of three PCR markers were significantly higher in the blood of patients with breast cancer than in healthy volunteers. Using the upper 95% confidence interval of cells detected in controls to determine positive patient samples: 30% of patients with metastatic breast cancer were positive following cell filtration, 48% following IMS, and 60, 45 and 35% using real-time RT–PCR for cytokeratin 19, mammaglobin and prolactin-inducible peptide. Samples were significantly more likely to be positive for at least one PCR marker than by cell filtration (83 vs 30%, P <0.001) or IMS (83 vs 48%, P <0.001). The use of a multimarker real-time RT–PCR assay was therefore found to be the most sensitive technique for the detection of circulating epithelial cells in the blood of patients with breast cancer.
AbstractList	This study compares the sensitivities and specificities of three techniques for the detection of circulating epithelial cells in the blood of patients with breast cancer. The number of circulating epithelial cells present in the blood of 40 patients with metastatic breast cancer and 20 healthy volunteers was determined by: immunomagnetic separation (IMS) and laser scanning cytometry (LSC), cell filtration and LSC and a multimarker real-time RT–PCR assay. Numbers of cytokeratin-positive cells identified and expression of three PCR markers were significantly higher in the blood of patients with breast cancer than in healthy volunteers. Using the upper 95% confidence interval of cells detected in controls to determine positive patient samples: 30% of patients with metastatic breast cancer were positive following cell filtration, 48% following IMS, and 60, 45 and 35% using real-time RT–PCR for cytokeratin 19, mammaglobin and prolactin-inducible peptide. Samples were significantly more likely to be positive for at least one PCR marker than by cell filtration (83 vs 30%, P <0.001) or IMS (83 vs 48%, P <0.001). The use of a multimarker real-time RT–PCR assay was therefore found to be the most sensitive technique for the detection of circulating epithelial cells in the blood of patients with breast cancer. This study compares the sensitivities and specificities of three techniques for the detection of circulating epithelial cells in the blood of patients with breast cancer. The number of circulating epithelial cells present in the blood of 40 patients with metastatic breast cancer and 20 healthy volunteers was determined by: immunomagnetic separation (IMS) and laser scanning cytometry (LSC), cell filtration and LSC and a multimarker real-time RT-PCR assay. Numbers of cytokeratin-positive cells identified and expression of three PCR markers were significantly higher in the blood of patients with breast cancer than in healthy volunteers. Using the upper 95% confidence interval of cells detected in controls to determine positive patient samples: 30% of patients with metastatic breast cancer were positive following cell filtration, 48% following IMS, and 60, 45 and 35% using real-time RT-PCR for cytokeratin 19, mammaglobin and prolactin-inducible peptide. Samples were significantly more likely to be positive for at least one PCR marker than by cell filtration (83 vs 30%, P<0.001) or IMS (83 vs 48%, P<0.001). The use of a multimarker real-time RT-PCR assay was therefore found to be the most sensitive technique for the detection of circulating epithelial cells in the blood of patients with breast cancer. This study compares the sensitivities and specificities of three techniques for the detection of circulating epithelial cells in the blood of patients with breast cancer. The number of circulating epithelial cells present in the blood of 40 patients with metastatic breast cancer and 20 healthy volunteers was determined by: immunomagnetic separation (IMS) and laser scanning cytometry (LSC), cell filtration and LSC and a multimarker real-time RT-PCR assay. Numbers of cytokeratin-positive cells identified and expression of three PCR markers were significantly higher in the blood of patients with breast cancer than in healthy volunteers. Using the upper 95% confidence interval of cells detected in controls to determine positive patient samples: 30% of patients with metastatic breast cancer were positive following cell filtration, 48% following IMS, and 60, 45 and 35% using real-time RT-PCR for cytokeratin 19, mammaglobin and prolactin-inducible peptide. Samples were significantly more likely to be positive for at least one PCR marker than by cell filtration (83 vs 30%, P<0.001) or IMS (83 vs 48%, P<0.001). The use of a multimarker real-time RT-PCR assay was therefore found to be the most sensitive technique for the detection of circulating epithelial cells in the blood of patients with breast cancer.This study compares the sensitivities and specificities of three techniques for the detection of circulating epithelial cells in the blood of patients with breast cancer. The number of circulating epithelial cells present in the blood of 40 patients with metastatic breast cancer and 20 healthy volunteers was determined by: immunomagnetic separation (IMS) and laser scanning cytometry (LSC), cell filtration and LSC and a multimarker real-time RT-PCR assay. Numbers of cytokeratin-positive cells identified and expression of three PCR markers were significantly higher in the blood of patients with breast cancer than in healthy volunteers. Using the upper 95% confidence interval of cells detected in controls to determine positive patient samples: 30% of patients with metastatic breast cancer were positive following cell filtration, 48% following IMS, and 60, 45 and 35% using real-time RT-PCR for cytokeratin 19, mammaglobin and prolactin-inducible peptide. Samples were significantly more likely to be positive for at least one PCR marker than by cell filtration (83 vs 30%, P<0.001) or IMS (83 vs 48%, P<0.001). The use of a multimarker real-time RT-PCR assay was therefore found to be the most sensitive technique for the detection of circulating epithelial cells in the blood of patients with breast cancer.
Author	Ring, A E Zabaglo, L Smith, I E Dowsett, M Ormerod, M G
Author_xml	– sequence: 1 givenname: A E surname: Ring fullname: Ring, A E organization: Academic Department of Biochemistry, Royal Marsden Hospital, Fulham Road, Breast Unit, Royal Marsden Hospital, Fulham Road – sequence: 2 givenname: L surname: Zabaglo fullname: Zabaglo, L organization: Academic Department of Biochemistry, Royal Marsden Hospital, Fulham Road – sequence: 3 givenname: M G surname: Ormerod fullname: Ormerod, M G organization: Academic Department of Biochemistry, Royal Marsden Hospital, Fulham Road – sequence: 4 givenname: I E surname: Smith fullname: Smith, I E organization: Breast Unit, Royal Marsden Hospital, Fulham Road – sequence: 5 givenname: M surname: Dowsett fullname: Dowsett, M email: mitch@icr.ac.uk organization: Academic Department of Biochemistry, Royal Marsden Hospital, Fulham Road
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Cites_doi	10.1200/JCO.1999.17.3.870 10.1080/0032472031000141283 10.1200/JCO.1999.17.7.2015 10.1002/(SICI)1097-0215(19990105)80:1<119::AID-IJC22>3.0.CO;2-X 10.1002/(SICI)1097-0215(19980610)76:6<824::AID-IJC10>3.0.CO;2-2 10.1038/sj.bjc.6690799 10.1177/172460089400900201 10.1016/S0002-9440(10)64706-2 10.1515/CCLM.2001.134 10.1016/S0959-8049(97)00014-2 10.1200/JCO.2000.18.7.1432 10.1073/pnas.95.8.4589 10.1016/S1470-2045(04)01381-6 10.1002/cyto.a.10071 10.1016/0277-5379(86)90077-5 10.1023/A:1008317512253 10.1093/annonc/mdf107 10.1200/JCO.2002.02.026 10.1200/JCO.2002.08.135 10.1023/A:1006261731125
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Keywords	breast cancer circulating tumour cells micrometastases Human Mammary gland diseases Blood cell Cancerology Epithelial cell Breast cancer Diagnosis Technique Malignant tumor Comparative study
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References	J Bartek (BF6602418_CR3) 1986; 22 JW Clark (BF6602418_CR6) 1999; 81 SE Singletary (BF6602418_CR18) 2002; 20 J Aerts (BF6602418_CR1) 2001; 12 AC Lambrechts (BF6602418_CR8) 1999; 56 BF6602418_CR9 A Stathopoulou (BF6602418_CR21) 2003; 9 O Zach (BF6602418_CR27) 1999; 17 A Stathopoulou (BF6602418_CR22) 2002; 20 K Pachmann (BF6602418_CR12) 2001; 39 MJ Slade (BF6602418_CR19) 1999; 17 BM Smith (BF6602418_CR20) 2000; 18 A Schoenfeld (BF6602418_CR16) 1997; 33 AJ Bosma (BF6602418_CR4) 2002; 8 B Brandt (BF6602418_CR5) 1998; 76 PY Muller (BF6602418_CR11) 2002; 32 L Zabaglo (BF6602418_CR26) 2003; 55A P Ruud (BF6602418_CR15) 1999; 80 R Moll (BF6602418_CR10) 1994; 9 AL Silva (BF6602418_CR17) 2002; 13 MK Baker (BF6602418_CR2) 2003; 9 E Racila (BF6602418_CR13) 1998; 95 B Taback (BF6602418_CR23) 2001; 61 G Vona (BF6602418_CR24) 2000; 156 TE Witzig (BF6602418_CR25) 2002; 8 BF6602418_CR7 A Ring (BF6602418_CR14) 2004; 5
References_xml	– volume: 17 start-page: 870 year: 1999 ident: BF6602418_CR19 publication-title: J Clin Oncol doi: 10.1200/JCO.1999.17.3.870 – ident: BF6602418_CR7 doi: 10.1080/0032472031000141283 – volume: 17 start-page: 2015 year: 1999 ident: BF6602418_CR27 publication-title: J Clin Oncol doi: 10.1200/JCO.1999.17.7.2015 – volume: 9 start-page: 4865 year: 2003 ident: BF6602418_CR2 publication-title: Clin Cancer Res – volume: 80 start-page: 119 year: 1999 ident: BF6602418_CR15 publication-title: Int J Cancer doi: 10.1002/(SICI)1097-0215(19990105)80:1<119::AID-IJC22>3.0.CO;2-X – volume: 76 start-page: 824 year: 1998 ident: BF6602418_CR5 publication-title: Int J Cancer doi: 10.1002/(SICI)1097-0215(19980610)76:6<824::AID-IJC10>3.0.CO;2-2 – volume: 81 start-page: 1002 year: 1999 ident: BF6602418_CR6 publication-title: Br J Cancer doi: 10.1038/sj.bjc.6690799 – ident: BF6602418_CR9 – volume: 9 start-page: 63 year: 1994 ident: BF6602418_CR10 publication-title: Int J Biol Markers doi: 10.1177/172460089400900201 – volume: 156 start-page: 57 year: 2000 ident: BF6602418_CR24 publication-title: Am J Pathol doi: 10.1016/S0002-9440(10)64706-2 – volume: 39 start-page: 811 year: 2001 ident: BF6602418_CR12 publication-title: Clin Chem Lab Med doi: 10.1515/CCLM.2001.134 – volume: 33 start-page: 854 year: 1997 ident: BF6602418_CR16 publication-title: Eur J Cancer doi: 10.1016/S0959-8049(97)00014-2 – volume: 8 start-page: 1085 year: 2002 ident: BF6602418_CR25 publication-title: Clin Cancer Res – volume: 18 start-page: 1432 year: 2000 ident: BF6602418_CR20 publication-title: J Clin Oncol doi: 10.1200/JCO.2000.18.7.1432 – volume: 95 start-page: 4589 year: 1998 ident: BF6602418_CR13 publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.95.8.4589 – volume: 5 start-page: 79 year: 2004 ident: BF6602418_CR14 publication-title: Lancet Oncol doi: 10.1016/S1470-2045(04)01381-6 – volume: 55A start-page: 102 year: 2003 ident: BF6602418_CR26 publication-title: Cytometry doi: 10.1002/cyto.a.10071 – volume: 32 start-page: 1372 year: 2002 ident: BF6602418_CR11 publication-title: Biotechniques – volume: 22 start-page: 1441 year: 1986 ident: BF6602418_CR3 publication-title: Eur J Cancer Clin Oncol doi: 10.1016/0277-5379(86)90077-5 – volume: 61 start-page: 8845 year: 2001 ident: BF6602418_CR23 publication-title: Cancer Res – volume: 12 start-page: 39 year: 2001 ident: BF6602418_CR1 publication-title: Ann Oncol doi: 10.1023/A:1008317512253 – volume: 13 start-page: 422 year: 2002 ident: BF6602418_CR17 publication-title: Ann Oncol doi: 10.1093/annonc/mdf107 – volume: 20 start-page: 3628 year: 2002 ident: BF6602418_CR18 publication-title: J Clin Oncol doi: 10.1200/JCO.2002.02.026 – volume: 20 start-page: 3404 year: 2002 ident: BF6602418_CR22 publication-title: J Clin Oncol doi: 10.1200/JCO.2002.08.135 – volume: 8 start-page: 1871 year: 2002 ident: BF6602418_CR4 publication-title: Clin Cancer Res – volume: 9 start-page: 5145 year: 2003 ident: BF6602418_CR21 publication-title: Clin Cancer Res – volume: 56 start-page: 219 year: 1999 ident: BF6602418_CR8 publication-title: Breast Cancer Res Treat doi: 10.1023/A:1006261731125
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Snippet	This study compares the sensitivities and specificities of three techniques for the detection of circulating epithelial cells in the blood of patients with...
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SubjectTerms	Adult Aged Base Sequence Biological and medical sciences Biomedical and Life Sciences Biomedicine Breast Neoplasms - blood Breast Neoplasms - pathology Cancer Research DNA Primers Drug Resistance Epidemiology Epithelial Cells - pathology Female Humans Keratins - analysis Lymphatic Metastasis Medical sciences Middle Aged Molecular Diagnostics Molecular Medicine Neoplasm Metastasis Neoplasm Staging Oncology Reference Values Reverse Transcriptase Polymerase Chain Reaction Tumors
Title	Detection of circulating epithelial cells in the blood of patients with breast cancer: comparison of three techniques
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