Enhancing prime editor activity by directed protein evolution in yeast

Prime editing is a highly versatile genome editing technology that enables the introduction of base substitutions, insertions, and deletions. However, compared to traditional Cas9 nucleases prime editors (PEs) are less active. In this study we use OrthoRep, a yeast-based platform for directed protei...

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Bibliographic Details
Published in:	Nature communications Vol. 15; no. 1; pp. 2092 - 11
Main Authors:	Weber, Yanik, Böck, Desirée, Ivașcu, Anastasia, Mathis, Nicolas, Rothgangl, Tanja, Ioannidi, Eleonora I., Blaudt, Alex C., Tidecks, Lisa, Vadovics, Máté, Muramatsu, Hiromi, Reichmuth, Andreas, Marquart, Kim F., Kissling, Lucas, Pardi, Norbert, Jinek, Martin, Schwank, Gerald
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 07.03.2024 Nature Publishing Group Nature Portfolio
Subjects:	42/109 42/41 42/70 45 45/23 45/77 45/88 49 631/208/4041/3196 631/337/4041/3196 631/61/201/2110 692/4017 82/83 Amino Acid Substitution Amino acids Animals Biological Assay Brain Cell Line Cell lines CRISPR-Cas Systems - genetics Editing Evolution Genome editing Genomes Humanities and Social Sciences In vivo methods and tests Mammals mRNA multidisciplinary Mutation Nuclease Proteins Saccharomyces cerevisiae - genetics Science Science (multidisciplinary) Substitutes Yeast
ISSN:	2041-1723, 2041-1723
Online Access:	Get full text
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