Effect of KnockOut serum replacement on germ cell development of immature testis tissue culture

To compare KnockOut serum replacement (KSR) and fetal bovine serum (FBS) for the development of germ cells. Testicular tissues from Sprague–Dawley rats were cultured for 4 weeks in culture media supplemented with FBS or KSR. Tissue area was measured at the beginning and end of the culturing period....

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Bibliographic Details
Published in:	Theriogenology Vol. 85; no. 2; pp. 193 - 199
Main Authors:	Liu, Feng, Cai, Chunhong, Wu, Xin, Cheng, Yanxia, Lin, Tao, Wei, Guanghui, He, Dawei
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 15.01.2016
Subjects:	Animals Apoptosis Cattle Culture Media Culture medium Fetal Blood fetal bovine serum Gene Expression genetic markers histology Male Rat Rats Rats, Sprague-Dawley reverse transcriptase polymerase chain reaction seminiferous tubules Seminiferous Tubules - anatomy & histology Serum Serum replacement spermatids Spermatids - cytology spermatocytes Spermatocytes - cytology Spermatogenesis Spermatogenesis - genetics spermatogonia Spermatogonia - cytology Spermatozoa - growth & development staining Testis Testis - cytology Testis - growth & development Tissue culture Tissue Culture Techniques tissues Culture medium Testis Tissue culture Rat Serum replacement Spermatogenesis
ISSN:	0093-691X, 1879-3231, 1879-3231
Online Access:	Get full text
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