Effect of KnockOut serum replacement on germ cell development of immature testis tissue culture

To compare KnockOut serum replacement (KSR) and fetal bovine serum (FBS) for the development of germ cells. Testicular tissues from Sprague–Dawley rats were cultured for 4 weeks in culture media supplemented with FBS or KSR. Tissue area was measured at the beginning and end of the culturing period....

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Veröffentlicht in:	Theriogenology Jg. 85; H. 2; S. 193 - 199
Hauptverfasser:	Liu, Feng, Cai, Chunhong, Wu, Xin, Cheng, Yanxia, Lin, Tao, Wei, Guanghui, He, Dawei
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States Elsevier Inc 15.01.2016
Schlagworte:	Animals Apoptosis Cattle Culture Media Culture medium Fetal Blood fetal bovine serum Gene Expression genetic markers histology Male Rat Rats Rats, Sprague-Dawley reverse transcriptase polymerase chain reaction seminiferous tubules Seminiferous Tubules - anatomy & histology Serum Serum replacement spermatids Spermatids - cytology spermatocytes Spermatocytes - cytology Spermatogenesis Spermatogenesis - genetics spermatogonia Spermatogonia - cytology Spermatozoa - growth & development staining Testis Testis - cytology Testis - growth & development Tissue culture Tissue Culture Techniques tissues Culture medium Testis Tissue culture Rat Serum replacement Spermatogenesis
ISSN:	0093-691X, 1879-3231, 1879-3231
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Abstract	To compare KnockOut serum replacement (KSR) and fetal bovine serum (FBS) for the development of germ cells. Testicular tissues from Sprague–Dawley rats were cultured for 4 weeks in culture media supplemented with FBS or KSR. Tissue area was measured at the beginning and end of the culturing period. Testicular histology, development of the germ cells, and the diameter of seminiferous tubules were analyzed by hematoxylin and eosin staining. After 4 weeks in culture, apoptosis and expression of the stage-specific spermatogenesis marker genes Kit, Sycp3, and Crisp1 were assayed. Tissues cultured in KSR-supplemented media were able to sustain growth and gradually increase seminiferous tubule diameter during the culture period. In addition, spermatogonia, primary spermatocytes, secondary spermatocytes, and round spermatids were observed after 4 weeks in culture, and reverse transcription-PCR confirmed expression of the marker genes. In comparison, tissues cultured in FBS-supplemented media showed dwindling testicular organization, necrotic seminiferous tubules, and expression of Kit, but inconsistent expression of Sycp3 and Crisp1 KnockOut serum replacement outperforms FBS as a growth media supplements for culturing immature spermatogonial tissue culture.
AbstractList	To compare KnockOut serum replacement (KSR) and fetal bovine serum (FBS) for the development of germ cells. Testicular tissues from Sprague–Dawley rats were cultured for 4 weeks in culture media supplemented with FBS or KSR. Tissue area was measured at the beginning and end of the culturing period. Testicular histology, development of the germ cells, and the diameter of seminiferous tubules were analyzed by hematoxylin and eosin staining. After 4 weeks in culture, apoptosis and expression of the stage-specific spermatogenesis marker genes Kit, Sycp3, and Crisp1 were assayed. Tissues cultured in KSR-supplemented media were able to sustain growth and gradually increase seminiferous tubule diameter during the culture period. In addition, spermatogonia, primary spermatocytes, secondary spermatocytes, and round spermatids were observed after 4 weeks in culture, and reverse transcription-PCR confirmed expression of the marker genes. In comparison, tissues cultured in FBS-supplemented media showed dwindling testicular organization, necrotic seminiferous tubules, and expression of Kit, but inconsistent expression of Sycp3 and Crisp1 KnockOut serum replacement outperforms FBS as a growth media supplements for culturing immature spermatogonial tissue culture. To compare KnockOut serum replacement (KSR) and fetal bovine serum (FBS) for the development of germ cells. Testicular tissues from Sprague-Dawley rats were cultured for 4 weeks in culture media supplemented with FBS or KSR. Tissue area was measured at the beginning and end of the culturing period. Testicular histology, development of the germ cells, and the diameter of seminiferous tubules were analyzed by hematoxylin and eosin staining. After 4 weeks in culture, apoptosis and expression of the stage-specific spermatogenesis marker genes Kit, Sycp3, and Crisp1 were assayed. Tissues cultured in KSR-supplemented media were able to sustain growth and gradually increase seminiferous tubule diameter during the culture period. In addition, spermatogonia, primary spermatocytes, secondary spermatocytes, and round spermatids were observed after 4 weeks in culture, and reverse transcription-PCR confirmed expression of the marker genes. In comparison, tissues cultured in FBS-supplemented media showed dwindling testicular organization, necrotic seminiferous tubules, and expression of Kit, but inconsistent expression of Sycp3 and Crisp1 KnockOut serum replacement outperforms FBS as a growth media supplements for culturing immature spermatogonial tissue culture. To compare KnockOut serum replacement (KSR) and fetal bovine serum (FBS) for the development of germ cells. Testicular tissues from Sprague-Dawley rats were cultured for 4 weeks in culture media supplemented with FBS or KSR. Tissue area was measured at the beginning and end of the culturing period. Testicular histology, development of the germ cells, and the diameter of seminiferous tubules were analyzed by hematoxylin and eosin staining. After 4 weeks in culture, apoptosis and expression of the stage-specific spermatogenesis marker genes Kit, Sycp3, and Crisp1 were assayed. Tissues cultured in KSR-supplemented media were able to sustain growth and gradually increase seminiferous tubule diameter during the culture period. In addition, spermatogonia, primary spermatocytes, secondary spermatocytes, and round spermatids were observed after 4 weeks in culture, and reverse transcription-PCR confirmed expression of the marker genes. In comparison, tissues cultured in FBS-supplemented media showed dwindling testicular organization, necrotic seminiferous tubules, and expression of Kit, but inconsistent expression of Sycp3 and Crisp1 KnockOut serum replacement outperforms FBS as a growth media supplements for culturing immature spermatogonial tissue culture.To compare KnockOut serum replacement (KSR) and fetal bovine serum (FBS) for the development of germ cells. Testicular tissues from Sprague-Dawley rats were cultured for 4 weeks in culture media supplemented with FBS or KSR. Tissue area was measured at the beginning and end of the culturing period. Testicular histology, development of the germ cells, and the diameter of seminiferous tubules were analyzed by hematoxylin and eosin staining. After 4 weeks in culture, apoptosis and expression of the stage-specific spermatogenesis marker genes Kit, Sycp3, and Crisp1 were assayed. Tissues cultured in KSR-supplemented media were able to sustain growth and gradually increase seminiferous tubule diameter during the culture period. In addition, spermatogonia, primary spermatocytes, secondary spermatocytes, and round spermatids were observed after 4 weeks in culture, and reverse transcription-PCR confirmed expression of the marker genes. In comparison, tissues cultured in FBS-supplemented media showed dwindling testicular organization, necrotic seminiferous tubules, and expression of Kit, but inconsistent expression of Sycp3 and Crisp1 KnockOut serum replacement outperforms FBS as a growth media supplements for culturing immature spermatogonial tissue culture.
Author	He, Dawei Lin, Tao Wei, Guanghui Liu, Feng Wu, Xin Cheng, Yanxia Cai, Chunhong
Author_xml	– sequence: 1 givenname: Feng surname: Liu fullname: Liu, Feng – sequence: 2 givenname: Chunhong surname: Cai fullname: Cai, Chunhong – sequence: 3 givenname: Xin surname: Wu fullname: Wu, Xin – sequence: 4 givenname: Yanxia surname: Cheng fullname: Cheng, Yanxia – sequence: 5 givenname: Tao surname: Lin fullname: Lin, Tao – sequence: 6 givenname: Guanghui surname: Wei fullname: Wei, Guanghui – sequence: 7 givenname: Dawei surname: He fullname: He, Dawei email: dr_daweihe@163.com
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Cites_doi	10.1017/S0967199403002363 10.1016/0014-4827(64)90156-9 10.4161/cc.5.11.2775 10.1016/j.semcdb.2014.04.021 10.1002/gene.20031 10.1095/biolreprod.102.014050 10.1210/jc.2010-2103 10.1002/j.1939-4640.1986.tb00873.x 10.1093/humrep/12.4.746 10.1016/B978-0-12-386602-8.50012-9 10.1111/j.2047-2927.2012.00008.x 10.1371/journal.pone.0077715 10.1016/j.semcdb.2014.04.007 10.1016/j.jbiotec.2013.11.028 10.1089/clo.2007.0085 10.1016/j.jcyt.2013.11.004 10.1093/humrep/dei275 10.1038/nature09850 10.1007/978-1-61779-794-1_1
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References	Steinberger, Steinberger (bib1) 1967; 2 Petkov, Anderson (bib19) 2008; 10 Zheng, Yu, Tian, Huang, Zhang (bib15) 2010; 15 Rey, Grinspon, Gottlieb, Pasqualini, Knoblovits, Aszpis (bib10) 2013; 1 de Mateo, Sassone-Corsi (bib11) 2014; 29 Cabrol, Ross, Fennoy, Bouvattier, Roger, Lahlou (bib9) 2011; 96 Sato, Katagiri, Gohbara, Inoue, Ogonuki, Ogura (bib14) 2011; 471 Li, Wang, Liu (bib17) 2014; 34 Cheng, Dutra, Takesono, Garrett-Beal, Schwartzberg (bib22) 2004; 39 Steinberger, Steinberger (bib2) 1970; 2 Nagano, Ryu, Brinster, Avarbock, Brinster (bib5) 2003; 68 Suzuki, Sato (bib6) 2003; 11 Anderson, Wallace, Groome, Bellis, Wu (bib7) 1997; 12 Hess, Cooke, Hofmann, Murphy (bib8) 2006; 5 Fraga, de Araújo, Stabellini, Vergani, Pereira (bib20) 2012; 873 Taha, Javeri, Kheirkhah, Majidizadeh, Khalatbary (bib21) 2014; 172 Janecki, Steinberger (bib13) 1986; 7 Hemeda, Giebel, Wagner (bib16) 2014; 16 Aoshima, Baba, Makino, Okada (bib18) 2013; 8 Steinberger, Steinberger, Perloff (bib3) 1964; 36 He, Li, Wei, Lin (bib4) 2006; 26 Hai, Hou, Liu, Liu, Yang, Li (bib12) 2014; 29 Peng, Chen (bib23) 2006; 21 Hai (10.1016/j.theriogenology.2015.09.012_bib12) 2014; 29 Peng (10.1016/j.theriogenology.2015.09.012_bib23) 2006; 21 Cabrol (10.1016/j.theriogenology.2015.09.012_bib9) 2011; 96 Sato (10.1016/j.theriogenology.2015.09.012_bib14) 2011; 471 Steinberger (10.1016/j.theriogenology.2015.09.012_bib1) 1967; 2 Hess (10.1016/j.theriogenology.2015.09.012_bib8) 2006; 5 Anderson (10.1016/j.theriogenology.2015.09.012_bib7) 1997; 12 Aoshima (10.1016/j.theriogenology.2015.09.012_bib18) 2013; 8 Taha (10.1016/j.theriogenology.2015.09.012_bib21) 2014; 172 de Mateo (10.1016/j.theriogenology.2015.09.012_bib11) 2014; 29 Rey (10.1016/j.theriogenology.2015.09.012_bib10) 2013; 1 Zheng (10.1016/j.theriogenology.2015.09.012_bib15) 2010; 15 Li (10.1016/j.theriogenology.2015.09.012_bib17) 2014; 34 Fraga (10.1016/j.theriogenology.2015.09.012_bib20) 2012; 873 Hemeda (10.1016/j.theriogenology.2015.09.012_bib16) 2014; 16 Steinberger (10.1016/j.theriogenology.2015.09.012_bib2) 1970; 2 Cheng (10.1016/j.theriogenology.2015.09.012_bib22) 2004; 39 Steinberger (10.1016/j.theriogenology.2015.09.012_bib3) 1964; 36 He (10.1016/j.theriogenology.2015.09.012_bib4) 2006; 26 Nagano (10.1016/j.theriogenology.2015.09.012_bib5) 2003; 68 Petkov (10.1016/j.theriogenology.2015.09.012_bib19) 2008; 10 Suzuki (10.1016/j.theriogenology.2015.09.012_bib6) 2003; 11 Janecki (10.1016/j.theriogenology.2015.09.012_bib13) 1986; 7
References_xml	– volume: 10 start-page: 263 year: 2008 end-page: 276 ident: bib19 article-title: Culture of porcine embryonic germ cells in serum-supplemented and serum-free conditions: the effects of serum and growth factors on primary and long-term culture publication-title: Cloning Stem Cells – volume: 5 start-page: 1164 year: 2006 end-page: 1170 ident: bib8 article-title: Mechanistic insights into the regulation of the spermatogonial stem cell niche publication-title: Cell Cycle – volume: 39 start-page: 100 year: 2004 end-page: 104 ident: bib22 article-title: Improved generation of C57BL/6J mouse embryonic stem cells in a defined serum-free media publication-title: Genesis – volume: 2 start-page: 117 year: 1967 end-page: 124 ident: bib1 article-title: Factors affecting spermatogenesis in organ cultures of mammalian testes publication-title: J Reprod Fertil – volume: 68 start-page: 2207 year: 2003 end-page: 2214 ident: bib5 article-title: Maintenance of mouse male germ line stem cells in vitro publication-title: Biol Reprod – volume: 16 start-page: 170 year: 2014 end-page: 180 ident: bib16 article-title: Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells publication-title: Cytotherapy – volume: 471 start-page: 504 year: 2011 end-page: 507 ident: bib14 article-title: In vitro production of functional sperm in cultured neonatal mouse testes publication-title: Nature – volume: 8 start-page: 77715 year: 2013 ident: bib18 article-title: Establishment of alternative culture method for spermatogonial stem cells using knockout serum replacement publication-title: PLoS One – volume: 7 start-page: 69 year: 1986 end-page: 71 ident: bib13 article-title: Polarized Sertoli cell functions in a new two-compartment culture system publication-title: J Andrology – volume: 172 start-page: 1 year: 2014 end-page: 10 ident: bib21 article-title: Neural differentiation of mouse embryonic and mesenchymal stem cells in a simple medium containing synthetic serum replacement publication-title: J Biotechnol – volume: 2 start-page: 363 year: 1970 end-page: 391 ident: bib2 article-title: In vitro growth and development of mammalian testes publication-title: Testis – volume: 36 start-page: 19 year: 1964 end-page: 27 ident: bib3 article-title: Mammalian testes in organ culture publication-title: Exp Cell Res – volume: 26 start-page: 323 year: 2006 end-page: 327 ident: bib4 article-title: Long-term proliferation characteristics of spermatogonial stem cells coculture with Sertoli cell layer in vitro publication-title: Reprod Contraception – volume: 29 start-page: 66 year: 2014 end-page: 75 ident: bib12 article-title: The roles and regulation of Sertoli cells in fate determinations of spermatogonial stem cells and spermatogenesis publication-title: Semin Cell Dev Biol – volume: 1 start-page: 3 year: 2013 end-page: 16 ident: bib10 article-title: Male hypogonadism: an extended classification based on a developmental, endocrine physiology-based approach publication-title: Andrology – volume: 29 start-page: 84 year: 2014 end-page: 92 ident: bib11 article-title: Regulation of spermatogenesis by small non-coding RNAs: role of the germ granule publication-title: Semin Cell Dev Biol – volume: 34 start-page: 751 year: 2014 end-page: 752 ident: bib17 article-title: Ultra-high performance liquid chromatography-mass spectrometry for analysis of newborn and fetal bovine serum components publication-title: Nan Fang Yi Ke Da Xue Xue Bao – volume: 21 start-page: 217 year: 2006 end-page: 222 ident: bib23 article-title: Serum-free medium cultivation to improve efficacy in establishment of human embryonic stem cell lines publication-title: Hum Reprod – volume: 11 start-page: 307 year: 2003 end-page: 316 ident: bib6 article-title: The fertilising ability of spermatogenic cells derived from cultured mouse immature testicular tissue publication-title: Zygote – volume: 12 start-page: 746 year: 1997 end-page: 751 ident: bib7 article-title: Physiological relationships between inhibin B, follicle stimulating hormone secretion and spermatogenesis in normal men and response to gonadotrophin suppression by exogenous testosterone publication-title: Hum Reprod – volume: 96 start-page: 746 year: 2011 end-page: 753 ident: bib9 article-title: Assessment of Leydig and Sertoli cell functions in infants with nonmosaic Klinefelter syndrome: insulin-like peptide 3 levels are normal and positively correlated with LH levels publication-title: J Clin Endocrinol Metab – volume: 15 start-page: 22 year: 2010 end-page: 24 ident: bib15 article-title: In vitro culture of calf testis tissue and examination of gene PGP9.5 expression publication-title: Chin J Anim Sci – volume: 873 start-page: 1 year: 2012 end-page: 12 ident: bib20 article-title: Establishment of new lines of human embryonic stem cells publication-title: Methods Mol Biol – volume: 11 start-page: 307 year: 2003 ident: 10.1016/j.theriogenology.2015.09.012_bib6 article-title: The fertilising ability of spermatogenic cells derived from cultured mouse immature testicular tissue publication-title: Zygote doi: 10.1017/S0967199403002363 – volume: 36 start-page: 19 year: 1964 ident: 10.1016/j.theriogenology.2015.09.012_bib3 article-title: Mammalian testes in organ culture publication-title: Exp Cell Res doi: 10.1016/0014-4827(64)90156-9 – volume: 5 start-page: 1164 year: 2006 ident: 10.1016/j.theriogenology.2015.09.012_bib8 article-title: Mechanistic insights into the regulation of the spermatogonial stem cell niche publication-title: Cell Cycle doi: 10.4161/cc.5.11.2775 – volume: 15 start-page: 22 year: 2010 ident: 10.1016/j.theriogenology.2015.09.012_bib15 article-title: In vitro culture of calf testis tissue and examination of gene PGP9.5 expression publication-title: Chin J Anim Sci – volume: 29 start-page: 84 year: 2014 ident: 10.1016/j.theriogenology.2015.09.012_bib11 article-title: Regulation of spermatogenesis by small non-coding RNAs: role of the germ granule publication-title: Semin Cell Dev Biol doi: 10.1016/j.semcdb.2014.04.021 – volume: 39 start-page: 100 year: 2004 ident: 10.1016/j.theriogenology.2015.09.012_bib22 article-title: Improved generation of C57BL/6J mouse embryonic stem cells in a defined serum-free media publication-title: Genesis doi: 10.1002/gene.20031 – volume: 68 start-page: 2207 year: 2003 ident: 10.1016/j.theriogenology.2015.09.012_bib5 article-title: Maintenance of mouse male germ line stem cells in vitro publication-title: Biol Reprod doi: 10.1095/biolreprod.102.014050 – volume: 96 start-page: 746 year: 2011 ident: 10.1016/j.theriogenology.2015.09.012_bib9 article-title: Assessment of Leydig and Sertoli cell functions in infants with nonmosaic Klinefelter syndrome: insulin-like peptide 3 levels are normal and positively correlated with LH levels publication-title: J Clin Endocrinol Metab doi: 10.1210/jc.2010-2103 – volume: 26 start-page: 323 year: 2006 ident: 10.1016/j.theriogenology.2015.09.012_bib4 article-title: Long-term proliferation characteristics of spermatogonial stem cells coculture with Sertoli cell layer in vitro publication-title: Reprod Contraception – volume: 7 start-page: 69 year: 1986 ident: 10.1016/j.theriogenology.2015.09.012_bib13 article-title: Polarized Sertoli cell functions in a new two-compartment culture system publication-title: J Andrology doi: 10.1002/j.1939-4640.1986.tb00873.x – volume: 12 start-page: 746 year: 1997 ident: 10.1016/j.theriogenology.2015.09.012_bib7 article-title: Physiological relationships between inhibin B, follicle stimulating hormone secretion and spermatogenesis in normal men and response to gonadotrophin suppression by exogenous testosterone publication-title: Hum Reprod doi: 10.1093/humrep/12.4.746 – volume: 2 start-page: 363 year: 1970 ident: 10.1016/j.theriogenology.2015.09.012_bib2 article-title: In vitro growth and development of mammalian testes publication-title: Testis doi: 10.1016/B978-0-12-386602-8.50012-9 – volume: 1 start-page: 3 year: 2013 ident: 10.1016/j.theriogenology.2015.09.012_bib10 article-title: Male hypogonadism: an extended classification based on a developmental, endocrine physiology-based approach publication-title: Andrology doi: 10.1111/j.2047-2927.2012.00008.x – volume: 8 start-page: 77715 year: 2013 ident: 10.1016/j.theriogenology.2015.09.012_bib18 article-title: Establishment of alternative culture method for spermatogonial stem cells using knockout serum replacement publication-title: PLoS One doi: 10.1371/journal.pone.0077715 – volume: 29 start-page: 66 year: 2014 ident: 10.1016/j.theriogenology.2015.09.012_bib12 article-title: The roles and regulation of Sertoli cells in fate determinations of spermatogonial stem cells and spermatogenesis publication-title: Semin Cell Dev Biol doi: 10.1016/j.semcdb.2014.04.007 – volume: 172 start-page: 1 year: 2014 ident: 10.1016/j.theriogenology.2015.09.012_bib21 article-title: Neural differentiation of mouse embryonic and mesenchymal stem cells in a simple medium containing synthetic serum replacement publication-title: J Biotechnol doi: 10.1016/j.jbiotec.2013.11.028 – volume: 10 start-page: 263 year: 2008 ident: 10.1016/j.theriogenology.2015.09.012_bib19 article-title: Culture of porcine embryonic germ cells in serum-supplemented and serum-free conditions: the effects of serum and growth factors on primary and long-term culture publication-title: Cloning Stem Cells doi: 10.1089/clo.2007.0085 – volume: 16 start-page: 170 year: 2014 ident: 10.1016/j.theriogenology.2015.09.012_bib16 article-title: Evaluation of human platelet lysate versus fetal bovine serum for culture of mesenchymal stromal cells publication-title: Cytotherapy doi: 10.1016/j.jcyt.2013.11.004 – volume: 21 start-page: 217 year: 2006 ident: 10.1016/j.theriogenology.2015.09.012_bib23 article-title: Serum-free medium cultivation to improve efficacy in establishment of human embryonic stem cell lines publication-title: Hum Reprod doi: 10.1093/humrep/dei275 – volume: 2 start-page: 117 year: 1967 ident: 10.1016/j.theriogenology.2015.09.012_bib1 article-title: Factors affecting spermatogenesis in organ cultures of mammalian testes publication-title: J Reprod Fertil – volume: 471 start-page: 504 year: 2011 ident: 10.1016/j.theriogenology.2015.09.012_bib14 article-title: In vitro production of functional sperm in cultured neonatal mouse testes publication-title: Nature doi: 10.1038/nature09850 – volume: 34 start-page: 751 year: 2014 ident: 10.1016/j.theriogenology.2015.09.012_bib17 article-title: Ultra-high performance liquid chromatography-mass spectrometry for analysis of newborn and fetal bovine serum components publication-title: Nan Fang Yi Ke Da Xue Xue Bao – volume: 873 start-page: 1 year: 2012 ident: 10.1016/j.theriogenology.2015.09.012_bib20 article-title: Establishment of new lines of human embryonic stem cells publication-title: Methods Mol Biol doi: 10.1007/978-1-61779-794-1_1
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Snippet	To compare KnockOut serum replacement (KSR) and fetal bovine serum (FBS) for the development of germ cells. Testicular tissues from Sprague–Dawley rats were... To compare KnockOut serum replacement (KSR) and fetal bovine serum (FBS) for the development of germ cells. Testicular tissues from Sprague-Dawley rats were...
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SubjectTerms	Animals Apoptosis Cattle Culture Media Culture medium Fetal Blood fetal bovine serum Gene Expression genetic markers histology Male Rat Rats Rats, Sprague-Dawley reverse transcriptase polymerase chain reaction seminiferous tubules Seminiferous Tubules - anatomy & histology Serum Serum replacement spermatids Spermatids - cytology spermatocytes Spermatocytes - cytology Spermatogenesis Spermatogenesis - genetics spermatogonia Spermatogonia - cytology Spermatozoa - growth & development staining Testis Testis - cytology Testis - growth & development Tissue culture Tissue Culture Techniques tissues
Title	Effect of KnockOut serum replacement on germ cell development of immature testis tissue culture
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