Moesin is a possible target molecule for cytomegalovirus-related Guillain-Barré syndrome

Previous histochemical studies in the demyelinating form of Guillain-Barré syndrome (GBS), acute inflammatory demyelinating polyneuropathy (AIDP), have shown complement deposition on the surface of Schwann cells, and therefore unknown epitopes would be present on the outer surface of Schwann cells....

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Veröffentlicht in:Neurology Jg. 83; H. 2; S. 113
Hauptverfasser: Sawai, Setsu, Satoh, Mamoru, Mori, Masahiro, Misawa, Sonoko, Sogawa, Kazuyuki, Kazami, Takahiro, Ishibashi, Masumi, Beppu, Minako, Shibuya, Kazumoto, Ishige, Takayuki, Sekiguchi, Yukari, Noda, Kenta, Sato, Kenichi, Matsushita, Kazuyuki, Kodera, Yoshio, Nomura, Fumio, Kuwabara, Satoshi
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 08.07.2014
Schlagworte:
Antigens, Viral - analysis
Blotting, Western
Cell Line, Tumor
Cytomegalovirus Infections - complications
Cytomegalovirus Infections - drug therapy
Electrodiagnosis
Electrophoresis, Agar Gel
Guillain-Barre Syndrome - drug therapy
Guillain-Barre Syndrome - etiology
Humans
Immunoglobulin G - analysis
Immunoglobulin M - analysis
Immunohistochemistry
Microfilament Proteins - drug effects
Neurilemmoma - pathology
Proteomics
Recombinant Proteins - analysis
ISSN:1526-632X, 1526-632X
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Zusammenfassung:Previous histochemical studies in the demyelinating form of Guillain-Barré syndrome (GBS), acute inflammatory demyelinating polyneuropathy (AIDP), have shown complement deposition on the surface of Schwann cells, and therefore unknown epitopes would be present on the outer surface of Schwann cells. We used a proteomic-based approach to search for the target molecules of AIDP in the extracted proteins from schwannoma cells. Sera were obtained from 40 patients with GBS, 31 controls with inflammatory disease, and 46 normal controls. We found that patients with AIDP after cytomegalovirus (CMV) infection have serum autoantibodies against membrane-organizing extension spike protein (moesin), which is expressed in the Schwann cell processes at the nodes of Ranvier and is crucial for myelination. Of the 40 patients with GBS, 6 had recent CMV infection and 5 of them (83%) had high levels of serum immunoglobulin G antibodies against moesin. The anti-moesin antibodies were found in none of the control subjects with disease including 5 with CMV infection but no neuropathy, and only 2 (4%) of the 46 normal control subjects. Immunocytochemistry showed that moesin was stained at the distal tips of schwannoma cells by sera from the patients with CMV-related AIDP but not by sera from controls. Moesin is a possible immunologic target molecule of pathogenic autoantibodies in patients with CMV-related AIDP. This study provides Class II evidence that levels of serum anti-moesin antibodies accurately distinguishes CMV-related AIDP from non-CMV-related AIDP (sensitivity 83%, specificity 93%).
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1526-632X
1526-632X
DOI:10.1212/WNL.0000000000000566