Evaluation of the QuantiFERON SARS-CoV-2 interferon-ɣ release assay in mRNA-1273 vaccinated health care workers

•44 % of vaccinees had INF-ɣ levels above 015 IU/mL in response to stimulation.•100 % of vaccinees showed INF-ɣ levels above 0015 IU/mL in response to stimulation.•More studies are needed to set an appropriate cut-off for this convenient assay. Current studies focus on cellular and humoral immunity...

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Published in:Journal of virological methods Vol. 298; p. 114295
Main Authors: Krüttgen, Alexander, Klingel, Hanna, Haase, Gerhard, Haefner, Helga, Imöhl, Matthias, Kleines, Michael
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01.12.2021
Published by Elsevier B.V
Subjects:
2019-nCoV Vaccine mRNA-1273
Antibodies, Viral
cell-mediated immunity
Cellular immunity
COVID-19
COVID-19 Vaccines
Health Personnel
health services
hepatitis B
Humans
humoral immunity
Interferon-gamma Release Tests
medicine
peptides
RNA, Messenger - genetics
SARS-CoV-2
Serology
Severe acute respiratory syndrome coronavirus 2
Vaccination
vaccines
COVID-19
SARS-CoV-2
Cellular immunity
Serology
Vaccination
ISSN:0166-0934, 1879-0984, 1879-0984
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Summary:•44 % of vaccinees had INF-ɣ levels above 015 IU/mL in response to stimulation.•100 % of vaccinees showed INF-ɣ levels above 0015 IU/mL in response to stimulation.•More studies are needed to set an appropriate cut-off for this convenient assay. Current studies focus on cellular and humoral immunity induced by novel SARS-CoV-2 vaccines. Non-responders to vaccinations are not uncommonly encountered in clinical medicine (e.g. in the field of hepatitis B). Whereas vaccine-induced humoral immunity against SARS-CoV-2 is compromised by emerging Variants of Concern (VOCs), cellular immunity against SARS-CoV-2 is emerging as resilient against VOCs. Thus commercially available test kits for diagnostic laboratories designed to evaluate cellular immune responses to SARS-CoV-2 are urgently needed. Here we evaluated the novel QuantiFERON SARS-CoV-2 assay (Qiagen) measuring INF-ɣ release induced by two spike-derived peptide pools (Ag1 and Ag2) in a cohort of health care workers vaccinated with the mRNA-1273 vaccine and confirmed humoral response. Our study indicates the usefulness of this novel assay for routine laboratories to evaluate cellular immunity against SARS-CoV-2 in response to mRNA-1273 vaccination.
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ISSN:0166-0934
1879-0984
1879-0984
DOI:10.1016/j.jviromet.2021.114295