Comparing the reprogramming efficiency of mouse embryonic fibroblasts, mouse bone marrow mesenchymal stem cells and bone marrow mononuclear cells to iPSCs

Induced pluripotent stem cells have been derived from various cell types via the ectopic expression of a cocktail of transcription factors. Previous studies have reported that induced pluripotent stem cells can be differentiated into multiple somatic cells, providing an invaluable resource in regene...

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Veröffentlicht in:In vitro cellular & developmental biology. Animal Jg. 48; H. 4; S. 236 - 243
Hauptverfasser: Wang, Lei, Zhu, Mingyan, Guo, Qingsong, Fan, Xiangjun, Lu, Yuhua, Zhu, Shajun, Wang, Yao, Huang, Yan, Wang, Zhiwei
Format: Journal Article
Sprache:Englisch
Veröffentlicht: New York Springer-Verlag 01.04.2012
Springer Science + Business Media
Society for In Vitro Biology
Schlagworte:
Alkaline phosphatase
Animal Genetics and Genomics
Animals
Antigens, CD
Antigens, CD - metabolism
Biomedical and Life Sciences
Blood cells
Bone marrow
Bone marrow cells
Bone Marrow Cells - cytology
Bone Marrow Cells - metabolism
CELL AND TISSUE MODELS
Cell Biology
Cell Culture
Cell Culture Techniques
Cell Differentiation
Cellular Reprogramming
clones
cytology
Developmental Biology
Embryo cells
Embryo fibroblasts
Embryonic stem cells
Enumeration
Feeder cells
fibroblasts
Fibroblasts - cytology
Induced pluripotent stem cells
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Inhibitory postsynaptic potentials
Leukocytes (mononuclear)
Life Sciences
medicine
Mesenchymal Stem Cells
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Mesenchyme
metabolism
Mice
Pluripotent Stem Cells
Pluripotent Stem Cells - cytology
Pluripotent Stem Cells - metabolism
Regeneration
Somatic cells
Stem Cells
T lymphocytes
teratoma
Transcription factors
iPSCs
BM MNCs
Pluripotency
BMSCs
Reprogramming efficiency
ISSN:1071-2690, 1543-706X, 1543-706X
Online-Zugang:Volltext
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Zusammenfassung:Induced pluripotent stem cells have been derived from various cell types via the ectopic expression of a cocktail of transcription factors. Previous studies have reported that induced pluripotent stem cells can be differentiated into multiple somatic cells, providing an invaluable resource in regenerative medicine. In this study, we compared the reprogramming efficiency of mouse embryonic fibroblasts, mouse bone marrow mesenchymal stem cells, and mouse bone marrow mononuclear cells by counting the number of alkaline phosphatase staining positive clones on day 15 after induced pluripotent stem cells induction. We found that a very low number of alkaline phosphatase-staining positive clones were derived from mouse bone marrow mesenchymal stem cells. We then evaluated the pluripotency of the clones by detecting the expression of embryonic stem cells markers and assessing their ability to form embryoid bodies and teratomas. Mouse bone marrow mesenchymal stem cells population is more homogeneous than mouse bone marrow mononuclear cells, which includes a variety of cell types. Our study indicated that the extremely low efficiency of mouse bone marrow mesenchymal stem cells induction implies that mouse bone marrow mesenchymal stem cells may not be a suitable cell type for the induction of induced pluripotent stem cells unless the efficiency of induction can be improved.
Bibliographie:http://dx.doi.org/10.1007/s11626-012-9493-0
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ISSN:1071-2690
1543-706X
1543-706X
DOI:10.1007/s11626-012-9493-0