The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: The HUMN project perspective on current status and knowledge gaps

The micronucleus (MN) assay in exfoliated buccal cells is a useful and minimally invasive method for monitoring genetic damage in humans. This overview has concluded that although MN assay in buccal cells has been used since the 1980s to demonstrate cytogenetic effects of environmental and occupatio...

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Vydáno v:Mutation research Ročník 659; číslo 1; s. 93 - 108
Hlavní autoři: Holland, Nina, Bolognesi, Claudia, Kirsch-Volders, Micheline, Bonassi, Stefano, Zeiger, Errol, Knasmueller, Siegfried, Fenech, Michael
Médium: Journal Article
Jazyk:angličtina
Vydáno: Lausanne Elsevier B.V 01.07.2008
Amsterdam Elsevier Science
New York, NY
Témata:
Biological and medical sciences
Biomarkers
Biomonitoring
Cancer
DNA Damage
Environmental exposure
Environmental Pollutants - toxicity
Evaluation Studies as Topic
Exfoliated cell
Forecasting
Fundamental and applied biological sciences. Psychology
Genotoxicity
Humans
Medical sciences
Metabolic diseases
Micronucleus Tests - methods
Micronucleus Tests - standards
Micronucleus Tests - trends
Molecular and cellular biology
Molecular genetics
Mouth Mucosa - cytology
Mutagenesis. Repair
Occupational exposure
Oral epithelia
Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...)
Precancerous lesion
Scoring criteria
Smoking
Tobacco, tobacco smoking
Toxicology
Validation Studies as Topic
Vitamin deficiency
Environmental exposure
Biomonitoring
Exfoliated cell
Genotoxicity
Biomarkers
Vitamin deficiency
Occupational exposure
Precancerous lesion
Scoring criteria
Cancer
Oral epithelia
Smoking
Toxicity
Tobacco smoking
Biological marker
Perspective
Micronucleus
Malnutrition
Nutritional status
GTPase activating protein
Human
Premalignant lesion
Mutagenicity testing
Nutrition disorder
Malignant tumor
Oral cavity
Metabolic disorder
DNA
Environment
ISSN:1383-5742, 0027-5107, 1388-2139
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Shrnutí:The micronucleus (MN) assay in exfoliated buccal cells is a useful and minimally invasive method for monitoring genetic damage in humans. This overview has concluded that although MN assay in buccal cells has been used since the 1980s to demonstrate cytogenetic effects of environmental and occupational exposures, lifestyle factors, dietary deficiencies, and different diseases, important knowledge gaps remain about the characteristics of micronuclei and other nuclear abnormalities, the basic biology explaining the appearance of various cell types in buccal mucosa samples and effects of diverse staining procedures and scoring criteria in laboratories around the world. To address these uncertainties, the human micronucleus project (HUMN; see http://www.humn.org) has initiated a new international validation project for the buccal cell MN assay similar to that previously performed using human lymphocytes. Future research should explore sources of variability in the assay (e.g. between laboratories and scorers, as well as inter- and intra-individual differences in subjects), and resolve key technical issues, such as the method of buccal cell staining, optimal criteria for classification of normal and degenerated cells and for scoring micronuclei and other abnormalities. The harmonization and standardization of the buccal MN assay will allow more reliable comparison of the data among human populations and laboratories, evaluation of the assay's performance, and consolidation of its world-wide use for biomonitoring of DNA damage.
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ISSN:1383-5742
0027-5107
1388-2139
DOI:10.1016/j.mrrev.2008.03.007