Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells

A streamlined, robust sample-preparation method for mass spectrometry–based proteome analysis is reported. All sample preparation steps are carried out in a single enclosed reactor, reducing the potential for contamination and losses, and enabling comprehensive proteome coverage. Mass spectrometry (...

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Bibliographic Details
Published in:	Nature methods Vol. 11; no. 3; pp. 319 - 324
Main Authors:	Kulak, Nils A, Pichler, Garwin, Paron, Igor, Nagaraj, Nagarjuna, Mann, Matthias
Format:	Journal Article
Language:	English
Published:	New York Nature Publishing Group US 01.03.2014 Nature Publishing Group
Subjects:	38/56 631/1647/2067 631/337/475 631/45/475 82/16 82/58 82/81 Bioinformatics Biological Microscopy Biological Techniques Biomedical Engineering/Biotechnology Cells Contamination DNA Contamination Eukaryotes Eukaryotic Cells - metabolism Gene Dosage - genetics HeLa Cells Humans Life Sciences Mass spectrometry Numbers Peptides Physiological aspects Proteomics Proteomics - methods Reproducibility of Results Saccharomyces cerevisiae Proteins - genetics Yeasts Germany
ISSN:	1548-7091, 1548-7105, 1548-7105
Online Access:	Get full text
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