Metabolomics in the Context of Plant Natural Products Research: From Sample Preparation to Metabolite Analysis

Plant-derived natural products have long been considered a valuable source of lead compounds for drug development. Natural extracts are usually composed of hundreds to thousands of metabolites, whereby the bioactivity of natural extracts can be represented by synergism between several metabolites. H...

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Veröffentlicht in:Metabolites Jg. 10; H. 1; S. 37
Hauptverfasser: Salem, Mohamed A., Perez de Souza, Leonardo, Serag, Ahmed, Fernie, Alisdair R., Farag, Mohamed A., Ezzat, Shahira M., Alseekh, Saleh
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Switzerland MDPI AG 15.01.2020
MDPI
Schlagworte:
Algorithms
Bioinformatics
Biological activity
Chromatography
Data analysis
Drug development
Drug discovery
Experiments
Fractionation
gas chromatography
liquid chromatography
Mass spectrometry
Mass spectroscopy
Membrane separation
Metabolism
metabolite extraction
Metabolites
Metabolomics
Natural products
NMR
Nuclear magnetic resonance
plant natural products
Plants
Quality control
Quantitative analysis
R&D
Research & development
Review
Scientific imaging
Secondary metabolites
Solvents
NMR
plant natural products
drug discovery
metabolomics
liquid chromatography
mass spectrometry
metabolite extraction
gas chromatography
ISSN:2218-1989, 2218-1989
Online-Zugang:Volltext
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Zusammenfassung:Plant-derived natural products have long been considered a valuable source of lead compounds for drug development. Natural extracts are usually composed of hundreds to thousands of metabolites, whereby the bioactivity of natural extracts can be represented by synergism between several metabolites. However, isolating every single compound from a natural extract is not always possible due to the complex chemistry and presence of most secondary metabolites at very low levels. Metabolomics has emerged in recent years as an indispensable tool for the analysis of thousands of metabolites from crude natural extracts, leading to a paradigm shift in natural products drug research. Analytical methods such as mass spectrometry (MS) and nuclear magnetic resonance (NMR) are used to comprehensively annotate the constituents of plant natural products for screening, drug discovery as well as for quality control purposes such as those required for phytomedicine. In this review, the current advancements in plant sample preparation, sample measurements, and data analysis are presented alongside a few case studies of the successful applications of these processes in plant natural product drug discovery.
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ISSN:2218-1989
2218-1989
DOI:10.3390/metabo10010037