Determination of clavulanic acid in calf plasma by liquid chromatography tandem mass spectrometry

A method for the quantification of clavulanic acid in calf plasma using high‐performance liquid chromatography combined with electrospray ionization (ESI) mass spectrometry, operating in the negative ionization mode (LC‐MS/MS), is presented. Sample preparation includes a simple and fast deproteiniza...

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Bibliographic Details
Published in:Journal of mass spectrometry. Vol. 41; no. 11; pp. 1414 - 1420
Main Authors: Reyns, Tim, De Baere, Siegrid, Croubels, Siska, De Backer, Patrick
Format: Journal Article
Language:English
Published: Chichester, UK John Wiley & Sons, Ltd 01.11.2006
Wiley
Subjects:
Administration, Oral
Amoxicillin - blood
Animal productions
Animals
Biological and medical sciences
Cattle
Chromatography, High Pressure Liquid
Chromatography, Liquid
clavulanic acid
Clavulanic Acid - administration & dosage
Clavulanic Acid - blood
Clavulanic Acid - pharmacokinetics
Fundamental and applied biological sciences. Psychology
liquid chromatography/electrospray ionization tandem mass spectrometry
pharmacokinetics
Reference Standards
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization
stability
Tandem Mass Spectrometry
Terrestrial animal productions
validation
Vertebrates
Biological fluid
Chemical analysis
Clavulanic acid
liquid chromatography/electrospray ionization tandem mass spectrometry
HPLC chromatography
Ox
β-Lactamase
Solvent extraction
Veterinary medicine
Electrospray
Blood
Blood plasma
Sample preparation
Ungulata
Validation
Farming animal
Stability
Enzyme
Liquid liquid extraction
Coupled method
Calf
Oral administration
Enzyme inhibitor
Vertebrata
Reversed phase chromatography
Mammalia
Animal
Mass spectrometry MS/MS
Hydrolases
Artiodactyla
Antibacterial agent
Pharmacokinetics
ISSN:1076-5174, 1096-9888
Online Access:Get full text
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Summary:A method for the quantification of clavulanic acid in calf plasma using high‐performance liquid chromatography combined with electrospray ionization (ESI) mass spectrometry, operating in the negative ionization mode (LC‐MS/MS), is presented. Sample preparation includes a simple and fast deproteinization with acetonitrile and a back‐extraction of the acetonitrile with dichloromethane. Chromatography is performed on a reversed‐phase PLRP‐S polymeric column using 0.05% formic acid in water and acetonitrile. The limit of quantification is 25 ng/ml, which is lower than other published methods using ultraviolet (UV), fluorimetric or mass spectrometric detection. The limit of detection is calculated to be 3.5 ng/ml. The stability of clavulanic acid was demonstrated according to The Guidelines of Bioanalytical Method Validation of The Food and Drug Administration (FDA): freeze and thaw stability, short‐term stability, long‐term stability, stock solution stability and postpreparative stability. The method is used in a pharmacokinetic and bioequivalence study of amoxycillin/clavulanic acid formulations in calves. Copyright © 2006 John Wiley & Sons, Ltd.
Bibliography:ark:/67375/WNG-2WNXMRFX-2
ArticleID:JMS1106
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ISSN:1076-5174
1096-9888
DOI:10.1002/jms.1106