Aptamer Switches Regulated by Post‐Transition/Transition Metal Ions

We introduced an aptamer switch design that relies on the ability of post‐transition/transition metal ions to trigger, through their coordination to nucleobases, substantial DNA destabilization. In the absence of molecular target, the addition of one such metal ion to usual aptamer working solutions...

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Vydáno v:Angewandte Chemie International Edition Ročník 60; číslo 22; s. 12346 - 12350
Hlavní autoři: Billet, Blandine, Chovelon, Benoit, Fiore, Emmanuelle, Oukacine, Farid, Petrillo, Mel‐Alexandre, Faure, Patrice, Ravelet, Corinne, Peyrin, Eric
Médium: Journal Article
Jazyk:angličtina
Vydáno: Germany Wiley Subscription Services, Inc 25.05.2021
Wiley-VCH Verlag
Vydání:International ed. in English
Témata:
Anisotropy
aptamer
Aptamers
Bases (nucleic acids)
Charge density
Chemical Sciences
Deoxyribonucleic acid
Destabilization
DNA
Fluorescence
fluorescence anisotropy
Lead
Medicinal Chemistry
metal ion
Metal ions
molecular target
structural switch
Surface charge
Switches
Transition metals
structural switch
metal ion
aptamer
fluorescence anisotropy
molecular target
ISSN:1433-7851, 1521-3773, 1521-3773
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Popis
Shrnutí:We introduced an aptamer switch design that relies on the ability of post‐transition/transition metal ions to trigger, through their coordination to nucleobases, substantial DNA destabilization. In the absence of molecular target, the addition of one such metal ion to usual aptamer working solutions promotes the formation of an alternative, inert DNA state. Upon exposure to the cognate compound, the equilibrium is shifted towards the competent DNA form. The switching process was preferentially activated by metal ions of intermediate base over phosphate complexation preference (i.e. Pb2+, Cd2+) and operated with diversely structured DNA molecules. This very simple aptamer switch scheme was applied to the detection of small organics using the fluorescence anisotropy readout mode. We envision that the approach could be adapted to a variety of signalling methods that report on changes in the surface charge density of DNA receptors. Structural transition metal ions: Post‐transition/transition metal ions can induce, through their coordination to nucleobases, substantial DNA destabilization. We exploited such property for elaborating fluorescent aptamer probes that switch between inert, metal ion‐complexed and active, target‐bound states. This very simple structural switching strategy was applied to the homogeneous‐phase detection of small organics using diversely structured aptamers.
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ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.202102254