Clearance of platelet microparticles in vivo

At present, little is known about the clearance of platelet-derived microparticles (PMP) in human blood, as due to ethical considerations infusion experiments with labeled microparticles are delicate. Therefore, we investigated the kinetics of PMP, which are abundantly present in apheresis platelet...

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Vydáno v:Platelets (Edinburgh) Ročník 22; číslo 2; s. 111 - 116
Hlavní autoři: Rank, A., Nieuwland, R., Crispin, A., Grützner, S., Iberer, M., Toth, B., Pihusch, R.
Médium: Journal Article
Jazyk:angličtina
Vydáno: England Informa UK Ltd 01.03.2011
Taylor & Francis
Témata:
Adolescent
Adult
Aged
Annexin A5 - metabolism
Antigens, CD - metabolism
Blood Platelets - metabolism
Cell-Derived Microparticles - metabolism
Female
Half-Life
half-life time
Humans
Kinetics
Male
Microparticle
Middle Aged
P-Selectin - metabolism
platelet concentrate
Platelet Count
Platelet Transfusion
Young Adult
ISSN:0953-7104, 1369-1635, 1369-1635
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Shrnutí:At present, little is known about the clearance of platelet-derived microparticles (PMP) in human blood, as due to ethical considerations infusion experiments with labeled microparticles are delicate. Therefore, we investigated the kinetics of PMP, which are abundantly present in apheresis platelet concentrates (PC), following platelet transfusion in severe thrombocytopenic patients (n = 11). PMP were double-stained with annexin V and cell-specific antibodies (anti-CD61, anti-CD63 or anti-CD62P, respectively) and detected by flow cytometry before and after transfusion of a single PC at fixed time intervals. Upon transfusion, the plasma levels of MP binding annexin V (2.5-fold), PMP (CD61+; 2.9-fold), and PMP from activated platelets (CD63+; 1.9-fold) or P-selectin (2.5-fold) increased immediately. The plasma levels of MP decreased with a half life of 5.8 hours (annexin V; 95% CI: 1.8-18.3) and 5.3 hours (CD61; 95% CI: 2.0-14.2). This is the first report in which the half life time of transfused PMP has been investigated in humans.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0953-7104
1369-1635
1369-1635
DOI:10.3109/09537104.2010.520373