A disposable biosensor for urea determination in blood based on an ammonium-sensitive transducer

A potentiometric urea-sensitive biosensor using a NH + 4-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is described. The ion-sensitive polymer matrix membrane was formed in the presence of an additional electrochemical inert filter paper matrix to improve th...

Full description

Saved in:

Bibliographic Details
Published in:	Biosensors & bioelectronics Vol. 14; no. 1; pp. 33 - 41
Main Authors:	Eggenstein, Claudia, Borchardt, Michael, Diekmann, Christoph, Bernd Gründig, Dumschat, Christa, Cammann, Karl, Knoll, Meinhard, Friedrich Spener
Format:	Journal Article
Language:	English
Published:	Lausanne Elsevier B.V 1999 Elsevier Science
Subjects:	Biological and medical sciences Biosensing Techniques Biosensors Biotechnology Blood Blood urea Disposable biosensor Disposable reference electrode Electrochemical electrodes Enzyme immobilization Fundamental and applied biological sciences. Psychology Humans Hydrogels Membranes, Artificial Methods. Procedures. Technologies Nitrogen compounds Polymeric membranes Polyurethanes Potentiometric biosensor Potentiometric sensors Potentiometry Quaternary Ammonium Compounds Transducers Urea Urea - blood Urea biosensor Various methods and equipments Urea biosensor Disposable reference electrode Potentiometric biosensor Disposable biosensor Blood urea Performance evaluation Disposable Enzyme Urease Potentiometry Enzyme electrode Blood Ion selective electrode Urea Biosensor Hydrolases Immobilized enzyme Medical application Quantitative analysis
ISSN:	0956-5663, 1873-4235
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Abstract	A potentiometric urea-sensitive biosensor using a NH + 4-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is described. The ion-sensitive polymer matrix membrane was formed in the presence of an additional electrochemical inert filter paper matrix to improve the reproducibility in sensor production. The electrodes were prepared from one-side silver-coated filter paper, which is encapsulated for insulation by a heat-sealing film. A defined volume of the NH + 4-sensitive polymer matrix membrane cocktail was deposited on this filter paper. To obtain the urea-biosensor a layer of urease was cast onto the ion-sensitive membrane. Poly (carbamoylsulfonate) hydrogel, produced from a hydrophilic polyurethane prepolymer blocked with bisulfite, served as immobilisation material. The disposable urea-sensitive electrode was combined with a disposable Ag/AgCl reference electrode to obtain the disposable urea biosensor. The sensor responded rapidly and in a stable manner to changes in urea concentrations between 7.2×10 −5 and 2.1×10 −2 mol/l. The detection limit was 2×10 −5 mol/l urea and the slope in the linear range 52 mV/decade. By taking into consideration the influence of the interfering K +- and Na +- ions the sensor can be used for the determination of urea in human blood and serum samples (diluted or undiluted). A good correlation was found with the data obtained by the spectrophotometric routine method.
AbstractList	A potentiometric urea-sensitive biosensor using a NH super(+) sub(4)-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is described. The ion-sensitive polymer matrix membrane was formed in the presence of an additional electrochemical inert filter paper matrix to improve the reproducibility in sensor production. The electrodes were prepared from one-side silver-coated filter paper, which is encapsulated for insulation by a heat-sealing film. A defined volume of the NH super(+) sub(4)-sensitive polymer matrix membrane cocktail was deposited on this filter paper. To obtain the urea-biosensor a layer of urease was cast onto the ion-sensitive membrane. Poly (carbamoylsulfonate) hydrogel, produced from a hydrophilic polyurethane prepolymer blocked with bisulfite, served as immobilization material. The disposable urea-sensitive electrode was combined with a disposable Ag/AgCl reference electrode to obtain the disposable urea biosensor. The sensor responded rapidly and in a stable manner to changes in urea concentrations between 7.2x10 super(-5) and 2.1x10 super(-2) mol/l. The detection limit was 2x10 super(-5) mol/l urea and the slope in the linear range 52 mV/decade. By taking into consideration the influence of the interfering K super(+)- and Na super(+)-ions the sensor can be used for the determination of urea in human blood and serum samples (diluted or undiluted). A good correlation was found with the data obtained by the spectrophotometric routine method. A potentiometric urea-sensitive biosensor using a NH4(+)-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is described. The ion-sensitive polymer matrix membrane was formed in the presence of an additional electrochemical inert filter paper matrix to improve the reproducibility in sensor production. The electrodes were prepared from one-side silver-coated filter paper, which is encapsulated for insulation by a heat-sealing film. A defined volume of the NH4(+)-sensitive polymer matrix membrane cocktail was deposited on this filter paper. To obtain the urea-biosensor a layer of urease was cast onto the ion-sensitive membrane. Poly (carbamoylsulfonate) hydrogel, produced from a hydrophilic polyurethane prepolymer blocked with bisulfite, served as immobilisation material. The disposable urea sensitive electrode was combined with a disposable Ag/AgCl reference electrode to obtain the disposable urea biosensor. The sensor responded rapidly and in a stable manner to changes in urea concentrations between 7.2 x 10(-5) and 2.1 x 10(-2)mol/l. The detection limit was 2 x 10(-5) mol/l urea and the slope in the linear range 52 mV/decade. By taking into consideration the influence of the interfering K(+)- and Na(+)-ions the sensor can be used for the determination of urea in human blood and serum samples (diluted or undiluted). A good correlation was found with the data obtained by the spectrophotometric routine method.A potentiometric urea-sensitive biosensor using a NH4(+)-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is described. The ion-sensitive polymer matrix membrane was formed in the presence of an additional electrochemical inert filter paper matrix to improve the reproducibility in sensor production. The electrodes were prepared from one-side silver-coated filter paper, which is encapsulated for insulation by a heat-sealing film. A defined volume of the NH4(+)-sensitive polymer matrix membrane cocktail was deposited on this filter paper. To obtain the urea-biosensor a layer of urease was cast onto the ion-sensitive membrane. Poly (carbamoylsulfonate) hydrogel, produced from a hydrophilic polyurethane prepolymer blocked with bisulfite, served as immobilisation material. The disposable urea sensitive electrode was combined with a disposable Ag/AgCl reference electrode to obtain the disposable urea biosensor. The sensor responded rapidly and in a stable manner to changes in urea concentrations between 7.2 x 10(-5) and 2.1 x 10(-2)mol/l. The detection limit was 2 x 10(-5) mol/l urea and the slope in the linear range 52 mV/decade. By taking into consideration the influence of the interfering K(+)- and Na(+)-ions the sensor can be used for the determination of urea in human blood and serum samples (diluted or undiluted). A good correlation was found with the data obtained by the spectrophotometric routine method. A potentiometric urea-sensitive biosensor using a NH4(+)-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is described. The ion-sensitive polymer matrix membrane was formed in the presence of an additional electrochemical inert filter paper matrix to improve the reproducibility in sensor production. The electrodes were prepared from one-side silver-coated filter paper, which is encapsulated for insulation by a heat-sealing film. A defined volume of the NH4(+)-sensitive polymer matrix membrane cocktail was deposited on this filter paper. To obtain the urea-biosensor a layer of urease was cast onto the ion-sensitive membrane. Poly (carbamoylsulfonate) hydrogel, produced from a hydrophilic polyurethane prepolymer blocked with bisulfite, served as immobilisation material. The disposable urea sensitive electrode was combined with a disposable Ag/AgCl reference electrode to obtain the disposable urea biosensor. The sensor responded rapidly and in a stable manner to changes in urea concentrations between 7.2 x 10(-5) and 2.1 x 10(-2)mol/l. The detection limit was 2 x 10(-5) mol/l urea and the slope in the linear range 52 mV/decade. By taking into consideration the influence of the interfering K(+)- and Na(+)-ions the sensor can be used for the determination of urea in human blood and serum samples (diluted or undiluted). A good correlation was found with the data obtained by the spectrophotometric routine method. A potentiometric urea-sensitive biosensor using a NH + 4-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is described. The ion-sensitive polymer matrix membrane was formed in the presence of an additional electrochemical inert filter paper matrix to improve the reproducibility in sensor production. The electrodes were prepared from one-side silver-coated filter paper, which is encapsulated for insulation by a heat-sealing film. A defined volume of the NH + 4-sensitive polymer matrix membrane cocktail was deposited on this filter paper. To obtain the urea-biosensor a layer of urease was cast onto the ion-sensitive membrane. Poly (carbamoylsulfonate) hydrogel, produced from a hydrophilic polyurethane prepolymer blocked with bisulfite, served as immobilisation material. The disposable urea-sensitive electrode was combined with a disposable Ag/AgCl reference electrode to obtain the disposable urea biosensor. The sensor responded rapidly and in a stable manner to changes in urea concentrations between 7.2×10 −5 and 2.1×10 −2 mol/l. The detection limit was 2×10 −5 mol/l urea and the slope in the linear range 52 mV/decade. By taking into consideration the influence of the interfering K +- and Na +- ions the sensor can be used for the determination of urea in human blood and serum samples (diluted or undiluted). A good correlation was found with the data obtained by the spectrophotometric routine method. A potentiometric urea-sensitive biosensor using a NH sub(4) super(+)-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is described. The ion-sensitive polymer matrix membrane was formed in the presence of an additional electrochemical inert filter paper matrix to improve the reproducibility in sensor production. The electrodes were prepared from one-side silver-coated filter paper, which is encapsulated for insulation by a heat-sealing film. A defined volume of the NH sub(4) super(+)-sensitive polymer matrix membrane cocktail was deposited on this filter paper. To obtain the urea-biosensor a layer of urease was cast onto the ion-sensitive membrane. Poly (carbamoylsulfonate) hydrogel, produced from a hydrophilic polyurethane prepolymer blocked with bisulfite, served as immobilisation material. The disposable urea-sensitive electrode was combined with a disposable Ag/AgCl reference electrode to obtain the disposable urea biosensor. The sensor responded rapidly and in a stable manner to changes in urea concentrations between 7.2 x 10 super(-5) and 2.1 x 10 super(-2) mol/l. The detection limit was 2 x 10 super(-5) mol/l urea and the slope in the linear range 52 mV/decade. By taking into consideration the influence of the interfering K super(+)- and Na super(+)- ions the sensor can be used for the determination of urea in human blood and serum samples (diluted or undiluted). A good correlation was found with the data obtained by the spectrophotometric routine method.
Author	Eggenstein, Claudia Bernd Gründig Knoll, Meinhard Dumschat, Christa Friedrich Spener Borchardt, Michael Diekmann, Christoph Cammann, Karl
Author_xml	– sequence: 1 givenname: Claudia surname: Eggenstein fullname: Eggenstein, Claudia organization: Institut für Chemo- und Biosensorik Münster, Mendelstr. 7, D-48149 Münster, Germany – sequence: 2 givenname: Michael surname: Borchardt fullname: Borchardt, Michael organization: Institut für Chemo- und Biosensorik Münster, Mendelstr. 7, D-48149 Münster, Germany – sequence: 3 givenname: Christoph surname: Diekmann fullname: Diekmann, Christoph organization: Institut für Chemo- und Biosensorik Münster, Mendelstr. 7, D-48149 Münster, Germany – sequence: 4 surname: Bernd Gründig fullname: Bernd Gründig organization: Institut für Chemo- und Biosensorik Münster, Mendelstr. 7, D-48149 Münster, Germany – sequence: 5 givenname: Christa surname: Dumschat fullname: Dumschat, Christa organization: Institut für Chemo- und Biosensorik Münster, Mendelstr. 7, D-48149 Münster, Germany – sequence: 6 givenname: Karl surname: Cammann fullname: Cammann, Karl organization: Institut für Chemo- und Biosensorik Münster, Mendelstr. 7, D-48149 Münster, Germany – sequence: 7 givenname: Meinhard surname: Knoll fullname: Knoll, Meinhard organization: Institut für Chemo- und Biosensorik Münster, Mendelstr. 7, D-48149 Münster, Germany – sequence: 8 surname: Friedrich Spener fullname: Friedrich Spener organization: Institut für Chemo- und Biosensorik Münster, Mendelstr. 7, D-48149 Münster, Germany
BackLink	http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1751676$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/10028647$$D View this record in MEDLINE/PubMed
BookMark	eNqFkkuLFTEQhYOMOHdGf4LSCxFdtFY6nUczCxkGXzDgQl3HdFKBSHdyTboH_PfmPnTAhRcSEsJ3Toqqc0HOYopIyFMKrylQ8eYLDFy0XAj2clCvACiwlj4gG6oka_uO8TOy-Yuck4tSfgCApAM8IucUoFOilxvy_bpxoWxTMeOEzRhSwVhSbnzda0bTOFwwzyGaJaTYhNiMU0quGU1B19QXU9c8pxjWud1pwxLusFmyicWtFvNj8tCbqeCT43lJvr1_9_XmY3v7-cOnm-vb1vaKLa2knQRpBB99ZxT33qrBMA-IQrpODMzR3nE_1gsH65zoeiFM54URwPqRs0vy4uC7zennimXRcygWp8lETGvRYuADVPQkWD_rFHB1GqSsV0rCSZDKTjEpaAWfHcF1nNHpbQ6zyb_0n3FU4PkRMMWaydcu2lDuOcmpkKJiVwfM5lRKRq9tWPYDqn0PU_XTu4zofUb0LgB6UHqfEb2rgv-jvq_j_7q3Bx3WMd4FzLrYgNGiCxntol0KJxx-Az3_0sI
CitedBy_id	crossref_primary_10_3390_s19132858 crossref_primary_10_1016_j_jelechem_2010_03_027 crossref_primary_10_1109_JSEN_2022_3232377 crossref_primary_10_1007_s10910_006_9102_0 crossref_primary_10_1002_adpr_202100058 crossref_primary_10_1016_j_bios_2017_09_005 crossref_primary_10_1016_S0925_4005_00_00410_X crossref_primary_10_1016_j_nanoen_2018_12_028 crossref_primary_10_1016_j_talanta_2014_10_064 crossref_primary_10_1016_j_colsurfb_2015_08_029 crossref_primary_10_3390_s16040474 crossref_primary_10_1080_00032710008543104 crossref_primary_10_1097_POC_0b013e3181cebd38 crossref_primary_10_3390_s18040942 crossref_primary_10_1039_C4AN01900H crossref_primary_10_1109_JSEN_2018_2850909 crossref_primary_10_1016_S0003_2670_02_00548_2 crossref_primary_10_1016_j_bios_2004_03_001 crossref_primary_10_1016_j_polymer_2009_05_030 crossref_primary_10_1002_1520_6017_200008_89_8_979__AID_JPS2_3_0_CO_2_H crossref_primary_10_1007_s12010_014_0985_0 crossref_primary_10_1016_j_bios_2007_07_006 crossref_primary_10_1016_S0927_7765_99_00148_4 crossref_primary_10_1016_j_bios_2020_112725 crossref_primary_10_1186_s42269_021_00649_0 crossref_primary_10_1016_S0021_9673_00_00173_4 crossref_primary_10_1081_AL_120017261 crossref_primary_10_1016_j_bej_2008_07_004 crossref_primary_10_1007_s00216_013_6912_3 crossref_primary_10_1088_2058_8585_aac8a8 crossref_primary_10_1016_j_aca_2017_04_039 crossref_primary_10_3390_chemosensors13030114 crossref_primary_10_1016_j_progpolymsci_2012_09_001 crossref_primary_10_1016_j_snb_2012_11_031 crossref_primary_10_1016_j_snb_2009_04_022 crossref_primary_10_1002_app_38860 crossref_primary_10_1016_j_electacta_2013_10_164 crossref_primary_10_1016_j_bios_2018_09_067 crossref_primary_10_1016_j_snb_2006_10_010 crossref_primary_10_1007_s12010_011_9348_2 crossref_primary_10_1016_j_bios_2014_10_075 crossref_primary_10_1016_S0003_2670_01_00891_1 crossref_primary_10_1016_j_aca_2005_02_011 crossref_primary_10_5194_jsss_7_559_2018 crossref_primary_10_1016_S0956_5663_02_00164_1 crossref_primary_10_1109_JSEN_2017_2751502 crossref_primary_10_3390_s18082607 crossref_primary_10_1016_j_mattod_2019_12_026 crossref_primary_10_1002_elan_200390008 crossref_primary_10_3390_s8095806 crossref_primary_10_1016_j_bios_2024_116850 crossref_primary_10_1002_ansa_202000074 crossref_primary_10_1002_jccs_201100386 crossref_primary_10_1007_s00542_025_05899_w crossref_primary_10_1007_s10854_024_13369_8 crossref_primary_10_1016_j_saa_2021_119556 crossref_primary_10_1016_j_bios_2012_07_022 crossref_primary_10_1016_j_bios_2017_01_023 crossref_primary_10_1016_j_bios_2006_10_007 crossref_primary_10_1016_j_bios_2003_11_028 crossref_primary_10_1038_s43246_024_00606_0 crossref_primary_10_1002_bit_28490 crossref_primary_10_1016_j_aca_2008_07_042 crossref_primary_10_1016_j_trac_2023_117345 crossref_primary_10_1016_j_bios_2015_10_009 crossref_primary_10_1016_j_jprot_2007_12_006 crossref_primary_10_1016_j_procbio_2006_01_017 crossref_primary_10_1134_S1070427216040017
Cites_doi	10.1016/0003-2670(92)80151-V 10.1016/0250-6874(85)87001-3 10.1016/0003-2670(86)80046-0 10.1016/0250-6874(87)80005-7 10.1021/ac50038a011 10.1016/S0003-2670(01)95405-4 10.1021/ja00711a052 10.1515/9783112719299 10.1021/ac60324a053 10.1016/0925-4005(95)85059-7 10.1016/0039-9140(94)E0107-3 10.1007/BF02447818 10.1007/BF00323930 10.1016/0302-4598(90)85008-6 10.1016/0925-4005(91)80019-G 10.1021/ac00097a011 10.1016/0022-0728(86)80186-3 10.1007/978-3-642-84038-8 10.1016/0925-4005(95)85159-3 10.1016/0956-5663(95)96935-R 10.1021/ja01036a083 10.1136/jcp.13.2.156
ContentType	Journal Article
Copyright	1999 Elsevier Science Ltd 1999 INIST-CNRS
Copyright_xml	– notice: 1999 Elsevier Science Ltd – notice: 1999 INIST-CNRS
DBID	AAYXX CITATION IQODW CGR CUY CVF ECM EIF NPM 7QO 8FD FR3 P64 7SP 7U5 L7M 7X8
DOI	10.1016/S0956-5663(98)00103-1
DatabaseName	CrossRef Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Biotechnology Research Abstracts Technology Research Database Engineering Research Database Biotechnology and BioEngineering Abstracts Electronics & Communications Abstracts Solid State and Superconductivity Abstracts Advanced Technologies Database with Aerospace MEDLINE - Academic
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Engineering Research Database Biotechnology Research Abstracts Technology Research Database Biotechnology and BioEngineering Abstracts Solid State and Superconductivity Abstracts Advanced Technologies Database with Aerospace Electronics & Communications Abstracts MEDLINE - Academic
DatabaseTitleList	Solid State and Superconductivity Abstracts MEDLINE - Academic MEDLINE Engineering Research Database
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Engineering Biology
EISSN	1873-4235
EndPage	41
ExternalDocumentID	379501 10028647 1751676 10_1016_S0956_5663_98_00103_1 S0956566398001031
Genre	Research Support, Non-U.S. Gov't Journal Article
GroupedDBID	--- --K --M .HR .~1 0R~ 1B1 1RT 1~. 1~5 23N 4.4 457 4G. 53G 5GY 5VS 7-5 71M 8P~ 9JM 9JN AABNK AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AARLI AAXUO ABFNM ABGSF ABJNI ABMAC ABUDA ABXDB ABYKQ ACDAQ ACGFS ACNNM ACRLP ADBBV ADECG ADEZE ADMUD ADTZH ADUVX AEBSH AECPX AEHWI AEKER AENEX AFFNX AFKWA AFTJW AFXIZ AFZHZ AGHFR AGRDE AGUBO AGYEJ AHHHB AHJVU AIEXJ AIKHN AITUG AJBFU AJOXV AJQLL AJSZI ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ASPBG AVWKF AXJTR AZFZN BJAXD BKOJK BLXMC CS3 DOVZS DU5 EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FLBIZ FNPLU FYGXN G-2 G-Q GBLVA HLW HMU HVGLF HZ~ IHE J1W JJJVA KOM LX3 M36 M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SBG SCB SCC SCH SDF SDG SDP SES SEW SPC SPCBC SSK SST SSU SSZ T5K TN5 WUQ XFK XPP Y6R YK3 ZMT ~G- ~KM 9DU AAHBH AATTM AAXKI AAYWO AAYXX ABWVN ACLOT ACRPL ACVFH ADCNI ADNMO AEIPS AEUPX AFJKZ AFPUW AGQPQ AIGII AIIUN AKBMS AKRWK AKYEP ANKPU APXCP CITATION EFKBS ~HD AGCQF AGRNS BNPGV IQODW SSH CGR CUY CVF ECM EIF NPM 7QO 8FD FR3 P64 7SP 7U5 L7M 7X8
ID	FETCH-LOGICAL-c483t-712707a65bf2a85ffc89a3f0ee67d2693d14d5fb93d50cdd62466a2f6a6034b53
ISICitedReferencesCount	92
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000078227100005&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	0956-5663
IngestDate	Sat Sep 27 17:05:11 EDT 2025 Sun Sep 28 00:53:56 EDT 2025 Sun Sep 28 09:38:06 EDT 2025 Mon Oct 06 18:05:41 EDT 2025 Wed Feb 19 01:20:55 EST 2025 Mon Jul 21 09:15:33 EDT 2025 Sat Nov 29 04:08:47 EST 2025 Tue Nov 18 19:56:08 EST 2025 Fri Feb 23 02:18:19 EST 2024
IsPeerReviewed	true
IsScholarly	true
Issue	1
Keywords	Urea biosensor Disposable reference electrode Potentiometric biosensor Disposable biosensor Blood urea Performance evaluation Disposable Enzyme Urease Potentiometry Enzyme electrode Blood Ion selective electrode Urea Biosensor Hydrolases Immobilized enzyme Medical application Quantitative analysis
Language	English
License	https://www.elsevier.com/tdm/userlicense/1.0 CC BY 4.0
LinkModel	OpenURL
MergedId	FETCHMERGED-LOGICAL-c483t-712707a65bf2a85ffc89a3f0ee67d2693d14d5fb93d50cdd62466a2f6a6034b53
Notes	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
PMID	10028647
PQID	17283761
PQPubID	23462
PageCount	9
ParticipantIDs	proquest_miscellaneous_69590034 proquest_miscellaneous_26928058 proquest_miscellaneous_21348870 proquest_miscellaneous_17283761 pubmed_primary_10028647 pascalfrancis_primary_1751676 crossref_citationtrail_10_1016_S0956_5663_98_00103_1 crossref_primary_10_1016_S0956_5663_98_00103_1 elsevier_sciencedirect_doi_10_1016_S0956_5663_98_00103_1
PublicationCentury	1900
PublicationDate	1999 1999-1-00 1999-Jan-01 19990101
PublicationDateYYYYMMDD	1999-01-01
PublicationDate_xml	– year: 1999 text: 1999
PublicationDecade	1990
PublicationPlace	Lausanne
PublicationPlace_xml	– name: Lausanne – name: England
PublicationTitle	Biosensors & bioelectronics
PublicationTitleAlternate	Biosens Bioelectron
PublicationYear	1999
Publisher	Elsevier B.V Elsevier Science
Publisher_xml	– name: Elsevier B.V – name: Elsevier Science
References	Karube, Tamiya, Dicks (BIB17) 1986; 185 (BIB16) 1976; 48 Vorlop, Muscat, Beyersdorf (BIB28) 1992; 6 Miyahara, Tsukada, Miyagi (BIB22) 1991; 3 Pindur, G., Pindur, U., 1991. Klinische Chemie und serologische Laboratoriumsdiagnostik. Wissenschaftliche Verlags GmbH, Stuttgart, pp. 244–309 Schindler, Schindler, Herna, Reisinger, Burk, Kuhlmann, Knaack, Schmidt, Langer (BIB26) 1994; 32 Guilbault, Montalvo (BIB13) 1970; 92 Rick, W., 1990. Klinische Chemie und Mikroskopie. Springer-Verlag, Berlin, pp. 245–247. Vadgama, Alberti, Covington (BIB27) 1982; 136 Bernt, E., Bergmeyer, U., 1970. Harnstoff. In: Bergmeyer, U. (Ed.), Methoden der enzymatischen Analyse, vol. 1. Verlag Chemie GmbH, Weinheim, pp. 1738–1748. Eggenstein, Borchardt, Dumschat, Gründig, Cammann, Spener, Knoll (BIB9) 1995; 10 Eddowes (BIB8) 1987; 11 Koncki, Leszcynski, Hulanicki, Glab (BIB19) 1992; 257 Ruzicka, Hansen, Ghose, Mottola (BIB25) 1979; 51 Knoll, M., 1991. German Patent No. P41 37 261.1. Borchardt, Dumschat, Cammann, Knoll (BIB4) 1995; 24/25 Campanella, Mazzei, Sammartino, Tomassetti (BIB5) 1990; 23 Borchardt, Diekmann, Dumschat, Cammann, Knoll (BIB3) 1994; 41 Fawcett, Scott (BIB10) 1960; 13 Miyahara, Moriizumi, Ichimura (BIB21) 1985; 7 Kotte, Gründig, Vorlop, Strehlitz, Stottmeister (BIB20) 1995; 67 Battaglia, C.J., Chang, J.C., Daniel, D.S., 1980. US Patent No. 4 214 968. Guilbault, Nagy (BIB14) 1973; 45 Diekmann, Dumschat, Cammann, Knoll (BIB6) 1995; 24/25 Dumschat, Borchardt, Diekmann, Hepke, Knoll (BIB7) 1994; 348 Hamann, Kühn, Böttcher, Scheller (BIB15) 1986; 209 Girndt, J., 1990. Nieren- und Hochdruckkrankheiten. Schattauer Verlags GmbH, Stuttgart, New York, pp. 57–236. Guilbault, Montalvo (BIB12) 1969; 91 Fawcett (10.1016/S0956-5663(98)00103-1_BIB10) 1960; 13 (10.1016/S0956-5663(98)00103-1_BIB16) 1976; 48 Eddowes (10.1016/S0956-5663(98)00103-1_BIB8) 1987; 11 Ruzicka (10.1016/S0956-5663(98)00103-1_BIB25) 1979; 51 Kotte (10.1016/S0956-5663(98)00103-1_BIB20) 1995; 67 10.1016/S0956-5663(98)00103-1_BIB18 Borchardt (10.1016/S0956-5663(98)00103-1_BIB3) 1994; 41 Dumschat (10.1016/S0956-5663(98)00103-1_BIB7) 1994; 348 10.1016/S0956-5663(98)00103-1_BIB11 Schindler (10.1016/S0956-5663(98)00103-1_BIB26) 1994; 32 Vadgama (10.1016/S0956-5663(98)00103-1_BIB27) 1982; 136 Campanella (10.1016/S0956-5663(98)00103-1_BIB5) 1990; 23 Eggenstein (10.1016/S0956-5663(98)00103-1_BIB9) 1995; 10 Miyahara (10.1016/S0956-5663(98)00103-1_BIB22) 1991; 3 Koncki (10.1016/S0956-5663(98)00103-1_BIB19) 1992; 257 Miyahara (10.1016/S0956-5663(98)00103-1_BIB21) 1985; 7 Guilbault (10.1016/S0956-5663(98)00103-1_BIB12) 1969; 91 Guilbault (10.1016/S0956-5663(98)00103-1_BIB13) 1970; 92 10.1016/S0956-5663(98)00103-1_BIB24 Diekmann (10.1016/S0956-5663(98)00103-1_BIB6) 1995; 24/25 10.1016/S0956-5663(98)00103-1_BIB23 Vorlop (10.1016/S0956-5663(98)00103-1_BIB28) 1992; 6 Hamann (10.1016/S0956-5663(98)00103-1_BIB15) 1986; 209 Borchardt (10.1016/S0956-5663(98)00103-1_BIB4) 1995; 24/25 Karube (10.1016/S0956-5663(98)00103-1_BIB17) 1986; 185 10.1016/S0956-5663(98)00103-1_BIB1 10.1016/S0956-5663(98)00103-1_BIB2 Guilbault (10.1016/S0956-5663(98)00103-1_BIB14) 1973; 45
References_xml	– volume: 48 start-page: 129 year: 1976 end-page: 132 ident: BIB16 article-title: Recommendations for nomenclature of ion-selective electrodes publication-title: Pure Appl. Chem. – volume: 257 start-page: 67 year: 1992 end-page: 72 ident: BIB19 article-title: Urea sensor based on glass pH electrodes with physically immobilized urease publication-title: Anal. Chim. Acta – volume: 45 start-page: 417 year: 1973 end-page: 419 ident: BIB14 article-title: Improved urea electrode publication-title: Anal. Chem. – volume: 136 start-page: 403 year: 1982 end-page: 406 ident: BIB27 article-title: Determination of urea in blood plasma by enzyme-pH electrode publication-title: Anal. Chim. Acta – reference: Knoll, M., 1991. German Patent No. P41 37 261.1. – volume: 3 start-page: 287 year: 1991 end-page: 293 ident: BIB22 article-title: Urea sensor based on an ammonium-ion-sensitive field-effect transistor publication-title: Sensors and Actuators B – reference: Battaglia, C.J., Chang, J.C., Daniel, D.S., 1980. US Patent No. 4 214 968. – volume: 185 start-page: 195 year: 1986 end-page: 200 ident: BIB17 article-title: A microsensor for urea based on an ion-selective field effect transistor publication-title: Anal. Chim. Acta – volume: 24/25 start-page: 276 year: 1995 end-page: 278 ident: BIB6 article-title: Disposable reference electrode publication-title: Sensors and Actuators B – volume: 24/25 start-page: 721 year: 1995 end-page: 723 ident: BIB4 article-title: Disposable ion selective electrodes publication-title: Sensors and Actuators B – volume: 209 start-page: 69 year: 1986 end-page: 76 ident: BIB15 article-title: Enzyme sensor system for potentiometric urea determination in serum and milk publication-title: J. Electroanal. Chem. – volume: 92 start-page: 2533 year: 1970 end-page: 2538 ident: BIB13 article-title: An enzyme electrode for the substrate urea publication-title: J. Am. Chem. Soc. – volume: 41 start-page: 1025 year: 1994 end-page: 1028 ident: BIB3 article-title: Disposable sodium electrodes publication-title: Talanta – volume: 11 start-page: 265 year: 1987 end-page: 274 ident: BIB8 article-title: Response of an enzyme-modified pH-sensitive ion-selective device: analytical solution for the response in the presence of pH buffer publication-title: Sensors and Actuators – volume: 91 start-page: 2164 year: 1969 end-page: 2165 ident: BIB12 article-title: Urea-specific enzyme electrode publication-title: J. Am. Chem. Soc. – volume: 6 start-page: 483 year: 1992 end-page: 488 ident: BIB28 article-title: Entrapment of microbial cells within polyurethane hydrogel beads with the advantage of low toxicity publication-title: J. Biotechnol. Technol. – volume: 32 start-page: 145 year: 1994 end-page: 152 ident: BIB26 article-title: Harnstoff-Durchflußsensor zur ammoniumselektiv-enzymatischen Prozeßkontrolle der künstlichen Niere publication-title: Eur. J. Clin. Chem. Clin. Biochem. – reference: Rick, W., 1990. Klinische Chemie und Mikroskopie. Springer-Verlag, Berlin, pp. 245–247. – reference: Pindur, G., Pindur, U., 1991. Klinische Chemie und serologische Laboratoriumsdiagnostik. Wissenschaftliche Verlags GmbH, Stuttgart, pp. 244–309 – volume: 23 start-page: 195 year: 1990 end-page: 202 ident: BIB5 article-title: New enzyme sensors for urea and creatinine analysis publication-title: Bioelectrochem. Bioenergetics – volume: 348 start-page: 553 year: 1994 end-page: 555 ident: BIB7 article-title: Double matrix membranes for potentiometric cation sensitive sensors publication-title: Fresenius J. Anal. Chem. – volume: 13 start-page: 156 year: 1960 end-page: 160 ident: BIB10 article-title: A rapid and precise method for the determination of urea publication-title: J. Clin. Pathol. – reference: Bernt, E., Bergmeyer, U., 1970. Harnstoff. In: Bergmeyer, U. (Ed.), Methoden der enzymatischen Analyse, vol. 1. Verlag Chemie GmbH, Weinheim, pp. 1738–1748. – reference: Girndt, J., 1990. Nieren- und Hochdruckkrankheiten. Schattauer Verlags GmbH, Stuttgart, New York, pp. 57–236. – volume: 7 start-page: 1 year: 1985 end-page: 10 ident: BIB21 article-title: Integrated enzyme FETs for simultaneous determination of urea and glucose publication-title: Sensors and Actuators – volume: 51 start-page: 199 year: 1979 end-page: 203 ident: BIB25 article-title: Enzymatic determination of urea in serum based on pH measurement with the flow injection method publication-title: Anal. Chem. – volume: 10 start-page: 595 year: 1995 end-page: 600 ident: BIB9 article-title: Potentiometric biosensor in double matrix membrane technology publication-title: Biosensors and Bioelectronics – volume: 67 start-page: 65 year: 1995 end-page: 70 ident: BIB20 article-title: Methylphenazonium-modified enzyme sensor based on polymer thick films for subnanomolar detection of phenols publication-title: Anal. Chem. – volume: 257 start-page: 67 year: 1992 ident: 10.1016/S0956-5663(98)00103-1_BIB19 article-title: Urea sensor based on glass pH electrodes with physically immobilized urease publication-title: Anal. Chim. Acta doi: 10.1016/0003-2670(92)80151-V – volume: 7 start-page: 1 year: 1985 ident: 10.1016/S0956-5663(98)00103-1_BIB21 article-title: Integrated enzyme FETs for simultaneous determination of urea and glucose publication-title: Sensors and Actuators doi: 10.1016/0250-6874(85)87001-3 – volume: 185 start-page: 195 year: 1986 ident: 10.1016/S0956-5663(98)00103-1_BIB17 article-title: A microsensor for urea based on an ion-selective field effect transistor publication-title: Anal. Chim. Acta doi: 10.1016/0003-2670(86)80046-0 – ident: 10.1016/S0956-5663(98)00103-1_BIB18 – volume: 11 start-page: 265 year: 1987 ident: 10.1016/S0956-5663(98)00103-1_BIB8 article-title: Response of an enzyme-modified pH-sensitive ion-selective device: analytical solution for the response in the presence of pH buffer publication-title: Sensors and Actuators doi: 10.1016/0250-6874(87)80005-7 – volume: 51 start-page: 199 year: 1979 ident: 10.1016/S0956-5663(98)00103-1_BIB25 article-title: Enzymatic determination of urea in serum based on pH measurement with the flow injection method publication-title: Anal. Chem. doi: 10.1021/ac50038a011 – volume: 48 start-page: 129 year: 1976 ident: 10.1016/S0956-5663(98)00103-1_BIB16 article-title: Recommendations for nomenclature of ion-selective electrodes publication-title: Pure Appl. Chem. – volume: 136 start-page: 403 year: 1982 ident: 10.1016/S0956-5663(98)00103-1_BIB27 article-title: Determination of urea in blood plasma by enzyme-pH electrode publication-title: Anal. Chim. Acta doi: 10.1016/S0003-2670(01)95405-4 – volume: 92 start-page: 2533 year: 1970 ident: 10.1016/S0956-5663(98)00103-1_BIB13 article-title: An enzyme electrode for the substrate urea publication-title: J. Am. Chem. Soc. doi: 10.1021/ja00711a052 – ident: 10.1016/S0956-5663(98)00103-1_BIB2 doi: 10.1515/9783112719299 – volume: 45 start-page: 417 year: 1973 ident: 10.1016/S0956-5663(98)00103-1_BIB14 article-title: Improved urea electrode publication-title: Anal. Chem. doi: 10.1021/ac60324a053 – volume: 24/25 start-page: 276 year: 1995 ident: 10.1016/S0956-5663(98)00103-1_BIB6 article-title: Disposable reference electrode publication-title: Sensors and Actuators B doi: 10.1016/0925-4005(95)85059-7 – volume: 41 start-page: 1025 year: 1994 ident: 10.1016/S0956-5663(98)00103-1_BIB3 article-title: Disposable sodium electrodes publication-title: Talanta doi: 10.1016/0039-9140(94)E0107-3 – volume: 6 start-page: 483 year: 1992 ident: 10.1016/S0956-5663(98)00103-1_BIB28 article-title: Entrapment of microbial cells within polyurethane hydrogel beads with the advantage of low toxicity publication-title: J. Biotechnol. Technol. doi: 10.1007/BF02447818 – ident: 10.1016/S0956-5663(98)00103-1_BIB1 – volume: 348 start-page: 553 year: 1994 ident: 10.1016/S0956-5663(98)00103-1_BIB7 article-title: Double matrix membranes for potentiometric cation sensitive sensors publication-title: Fresenius J. Anal. Chem. doi: 10.1007/BF00323930 – volume: 23 start-page: 195 year: 1990 ident: 10.1016/S0956-5663(98)00103-1_BIB5 article-title: New enzyme sensors for urea and creatinine analysis publication-title: Bioelectrochem. Bioenergetics doi: 10.1016/0302-4598(90)85008-6 – ident: 10.1016/S0956-5663(98)00103-1_BIB11 – volume: 3 start-page: 287 year: 1991 ident: 10.1016/S0956-5663(98)00103-1_BIB22 article-title: Urea sensor based on an ammonium-ion-sensitive field-effect transistor publication-title: Sensors and Actuators B doi: 10.1016/0925-4005(91)80019-G – volume: 67 start-page: 65 year: 1995 ident: 10.1016/S0956-5663(98)00103-1_BIB20 article-title: Methylphenazonium-modified enzyme sensor based on polymer thick films for subnanomolar detection of phenols publication-title: Anal. Chem. doi: 10.1021/ac00097a011 – volume: 209 start-page: 69 year: 1986 ident: 10.1016/S0956-5663(98)00103-1_BIB15 article-title: Enzyme sensor system for potentiometric urea determination in serum and milk publication-title: J. Electroanal. Chem. doi: 10.1016/0022-0728(86)80186-3 – ident: 10.1016/S0956-5663(98)00103-1_BIB24 doi: 10.1007/978-3-642-84038-8 – volume: 24/25 start-page: 721 year: 1995 ident: 10.1016/S0956-5663(98)00103-1_BIB4 article-title: Disposable ion selective electrodes publication-title: Sensors and Actuators B doi: 10.1016/0925-4005(95)85159-3 – volume: 32 start-page: 145 year: 1994 ident: 10.1016/S0956-5663(98)00103-1_BIB26 article-title: Harnstoff-Durchflußsensor zur ammoniumselektiv-enzymatischen Prozeßkontrolle der künstlichen Niere publication-title: Eur. J. Clin. Chem. Clin. Biochem. – volume: 10 start-page: 595 year: 1995 ident: 10.1016/S0956-5663(98)00103-1_BIB9 article-title: Potentiometric biosensor in double matrix membrane technology publication-title: Biosensors and Bioelectronics doi: 10.1016/0956-5663(95)96935-R – ident: 10.1016/S0956-5663(98)00103-1_BIB23 – volume: 91 start-page: 2164 year: 1969 ident: 10.1016/S0956-5663(98)00103-1_BIB12 article-title: Urea-specific enzyme electrode publication-title: J. Am. Chem. Soc. doi: 10.1021/ja01036a083 – volume: 13 start-page: 156 year: 1960 ident: 10.1016/S0956-5663(98)00103-1_BIB10 article-title: A rapid and precise method for the determination of urea publication-title: J. Clin. Pathol. doi: 10.1136/jcp.13.2.156
SSID	ssj0007190
Score	1.8861362
Snippet	A potentiometric urea-sensitive biosensor using a NH + 4-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is described.... A potentiometric urea-sensitive biosensor using a NH4(+)-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is described.... A potentiometric urea-sensitive biosensor using a NH sub(4) super(+)-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is... A potentiometric urea-sensitive biosensor using a NH super(+) sub(4)-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is...
SourceID	proquest pubmed pascalfrancis crossref elsevier
SourceType	Aggregation Database Index Database Enrichment Source Publisher
StartPage	33
SubjectTerms	Biological and medical sciences Biosensing Techniques Biosensors Biotechnology Blood Blood urea Disposable biosensor Disposable reference electrode Electrochemical electrodes Enzyme immobilization Fundamental and applied biological sciences. Psychology Humans Hydrogels Membranes, Artificial Methods. Procedures. Technologies Nitrogen compounds Polymeric membranes Polyurethanes Potentiometric biosensor Potentiometric sensors Potentiometry Quaternary Ammonium Compounds Transducers Urea Urea - blood Urea biosensor Various methods and equipments
Title	A disposable biosensor for urea determination in blood based on an ammonium-sensitive transducer
URI	https://dx.doi.org/10.1016/S0956-5663(98)00103-1 https://www.ncbi.nlm.nih.gov/pubmed/10028647 https://www.proquest.com/docview/17283761 https://www.proquest.com/docview/21348870 https://www.proquest.com/docview/26928058 https://www.proquest.com/docview/69590034
Volume	14
WOSCitedRecordID	wos000078227100005&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVESC databaseName: Elsevier SD Freedom Collection Journals 2021 customDbUrl: eissn: 1873-4235 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0007190 issn: 0956-5663 databaseCode: AIEXJ dateStart: 19950101 isFulltext: true titleUrlDefault: https://www.sciencedirect.com providerName: Elsevier
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1ti9NAEF56PQVFRM9Tq96ZDwpKiTabZF8-1uNERQ7BU_ot5m0l2EtK0x7n7_IPOrObZFtLPRWEEsKyL2nmye4zs7MzhDxRoJlkgmI8bSrdQCjlJgkDZUUiemKlYm3K_vyen5yIyUR-6PV-tGdhzqe8LMXFhZz9V1FDGQgbj87-hbi7TqEA7kHocAWxw_WPBD_GTZdZVeszUUlR1aCoVnPtToge6MOsdYBp3Ry17_oQl7NsqH2ThzE-bbE8c7GtcS5a4JqWAQ7ma9vAbfe1hhCMZtPqWLL-FZ61brNqHk3jZVZYM0ClgzVli199-JFcF_m3syaJcxcEoWuYz0u0peFO_6sjjDZrDRgY9MDa0zbO1DSGSeYCyTTTXm6mZcF9F4hfuDZvBxv4NJOw768s5yas1sZCYWwWH7vBgM5L8ZRKnffC9ezq2Pks6rpYVQqTHGOH7FIeStEnu-O3x5N3HQHgnjHttX3bg2Mv7YDPpHjeDLaNEt2YxTV8qMpkWNmuAmkqdHqL3Gx0GGdssHeb9PJyj1w1WU2_75HrKzEu75AvY8fi0enw6AAeHcSjs4ZHpygdjUdH49GBkhh-G3h0LB73yafXx6dHb9wmrYebBsJfuBydHXjMwkTRWIRKpULGvhrlOeMZZdLPvCALVQI34SjNMkYDxmKqWMxGfpCE_l3SL6syv08cltKQJj4XmBaCqVB4KkgUD6gC7idEOCBB-2ajtIl5j6lXppF1bgSBRCiQSIpICyTyBuRF12xmgr5c1kC0Yosa5moYaQR4u6zpwZqY7YA89BhnA_K4FXsEMz9u58VlXi3rCDPLAT3wttfAaI1AIka_qcEkFaNQbK_BJOYV9oMBuWcwt_JGQDdhAX_w73_-IblmwqCgSfMR6S_my_yAXEnPF0U9PyQ7fCIOm6_rJ8Zw_Ns
linkProvider	Elsevier
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+disposable+biosensor+for+urea+determination+in+blood+based+on+an+ammonium-sensitive+transducer&rft.jtitle=Biosensors+%26+bioelectronics&rft.au=Eggenstein%2C+Claudia&rft.au=Borchardt%2C+Michael&rft.au=Diekmann%2C+Christoph&rft.au=Bernd+Gr%C3%BCndig&rft.date=1999&rft.pub=Elsevier+B.V&rft.issn=0956-5663&rft.eissn=1873-4235&rft.volume=14&rft.issue=1&rft.spage=33&rft.epage=41&rft_id=info:doi/10.1016%2FS0956-5663%2898%2900103-1&rft.externalDocID=S0956566398001031
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0956-5663&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0956-5663&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0956-5663&client=summon