A disposable biosensor for urea determination in blood based on an ammonium-sensitive transducer

A potentiometric urea-sensitive biosensor using a NH + 4-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is described. The ion-sensitive polymer matrix membrane was formed in the presence of an additional electrochemical inert filter paper matrix to improve th...

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Vydáno v:Biosensors & bioelectronics Ročník 14; číslo 1; s. 33 - 41
Hlavní autoři: Eggenstein, Claudia, Borchardt, Michael, Diekmann, Christoph, Bernd Gründig, Dumschat, Christa, Cammann, Karl, Knoll, Meinhard, Friedrich Spener
Médium: Journal Article
Jazyk:angličtina
Vydáno: Lausanne Elsevier B.V 1999
Elsevier Science
Témata:
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
Blood
Blood urea
Disposable biosensor
Disposable reference electrode
Electrochemical electrodes
Enzyme immobilization
Fundamental and applied biological sciences. Psychology
Humans
Hydrogels
Membranes, Artificial
Methods. Procedures. Technologies
Nitrogen compounds
Polymeric membranes
Polyurethanes
Potentiometric biosensor
Potentiometric sensors
Potentiometry
Quaternary Ammonium Compounds
Transducers
Urea
Urea - blood
Urea biosensor
Various methods and equipments
Urea biosensor
Disposable reference electrode
Potentiometric biosensor
Disposable biosensor
Blood urea
Performance evaluation
Disposable
Enzyme
Urease
Potentiometry
Enzyme electrode
Blood
Ion selective electrode
Urea
Biosensor
Hydrolases
Immobilized enzyme
Medical application
Quantitative analysis
ISSN:0956-5663, 1873-4235
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Shrnutí:A potentiometric urea-sensitive biosensor using a NH + 4-sensitive disposable electrode in double matrix membrane (DMM) technology as transducer is described. The ion-sensitive polymer matrix membrane was formed in the presence of an additional electrochemical inert filter paper matrix to improve the reproducibility in sensor production. The electrodes were prepared from one-side silver-coated filter paper, which is encapsulated for insulation by a heat-sealing film. A defined volume of the NH + 4-sensitive polymer matrix membrane cocktail was deposited on this filter paper. To obtain the urea-biosensor a layer of urease was cast onto the ion-sensitive membrane. Poly (carbamoylsulfonate) hydrogel, produced from a hydrophilic polyurethane prepolymer blocked with bisulfite, served as immobilisation material. The disposable urea-sensitive electrode was combined with a disposable Ag/AgCl reference electrode to obtain the disposable urea biosensor. The sensor responded rapidly and in a stable manner to changes in urea concentrations between 7.2×10 −5 and 2.1×10 −2 mol/l. The detection limit was 2×10 −5 mol/l urea and the slope in the linear range 52 mV/decade. By taking into consideration the influence of the interfering K +- and Na +- ions the sensor can be used for the determination of urea in human blood and serum samples (diluted or undiluted). A good correlation was found with the data obtained by the spectrophotometric routine method.
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ISSN:0956-5663
1873-4235
DOI:10.1016/S0956-5663(98)00103-1